Pearl® Trilogy Accessories Manual CE Marking: This product (model number 5700-DS) is a CE-marked product. For conformity information, contact LI-COR Support at http://www.licor.com/biotechsupport. Outside of the U.S., contact your local sales office or distributor. Notes on Safety LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this product cannot be guaranteed if the product is used in any other way than is specified in this manual. The Pearl® Docking Station and Pearl Clean Box is intended to be used by qualified personnel. Read this entire manual before using the Pearl Docking Station and Pearl Clean Box. Equipment Markings: The product is marked with this symbol when it is necessary for you to refer to the manual or accompanying documents in order to protect against damage to the product. The product is marked with this symbol when a hazardous voltage may be present. Manual Markings: WARNING Warnings must be followed carefully to avoid bodily injury. CAUTION Cautions must be observed to avoid damaging your equipment. NOTE Notes contain additional information and useful tips. IMPORTANT Information of importance to prevent procedural mistakes in the operation of the equipment or related software. Failure to comply may result in a poor experimental outcome but will not cause bodily injury or equipment damage. Federal Communications Commission Radio Frequency Interference Statement WARNING: This equipment generates, uses, and can radiate radio frequency energy and if not installed in accordance with the instruction manual, may cause interference to radio communications. It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of FCC rules, which are designed to provide a reasonable protection against such interference when operated in a commercial environment. Operation of this equipment in a residential area is likely to cause interference in which case the user, at his own expense, will be required to take whatever measures may be required to correct the interference. Page iii Notice The information contained in this document is subject to change without notice. LI-COR MAKES NO WARRANTY OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. LI-COR shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of this material. This document contains proprietary information that is protected by copyright. All rights are reserved. No part of this document may be photocopied, reproduced, or translated to another language without prior written consent of LI-COR, Inc. Printing History Publication Number 977-19833 Printed: February 2015 Revised: September 2021 LI-COR Biosciences is an ISO 9001:2015 certified company. © 2021 LI-COR, Inc. Specifications subject to change. LI-COR, Pearl, In-Cell Western, IRDye, Odyssey, and Image Studio are registered trademarks of LI-COR, Inc. in the United States and other countries. All other trademarks belong to their respective owners. The Odyssey Infrared Imaging Systems and IRDye reagents are covered by U.S. patents, foreign equivalents, and patents pending. Page iv Pearl® Imager Table of Contents Accessories Chapter 1: Pearl Docking Station Description 1 Assembling the Pearl Docking Station 2 Docking Station Operation 6 Routine Maintenance 8 Docking Station Specifications 8 Chapter 2: Pearl Clean Box Description 9 Installation 9 Connecting a HEPA Filter to the Docking Station 9 Connecting a HEPA Filter to the Pearl® Imager 10 Nose Cone Installation and Replacement 10 Sanitizing the Pearl Clean Box 11 Clean Box Operation 11 Maintenance of the Clean Box Window 12 Scratches 12 Smudging 12 Chemicals 13 Cleaning Procedure 13 Replacing Heater Plate Contact Pins 13 Page v Pearl® Imager Accessories Chapter 1: Pearl Docking Station Description The Pearl Docking Station is an optional, external accessory that duplicates many of the features of the Pearl® Imager imaging drawer. The main features and functions of the Docking Station are listed below. l Duplicates the docking hardware in the Pearl Imager imaging drawer, allowing an access- ory imaging bed to be locked in place on the docking station similar to the Pearl imaging drawer. l Provides temperature and anesthesia gas con- trol to a Pearl accessory imaging bed outside of the Pearl Imager, allowing animals to be prepared for imaging at an external station. l Warms the heater plate in any of the Pearl accessory imaging beds (when docked) to 38 °C so animals can be placed on warm ima- Figure 1-1. Pearl Docking Station (left) with optional ging beds before transfer to the Pearl Imager. Pearl Clean Box (mounted) and isoflurane vaporizer (right) of the SmartFlow Anesthesia System. l Provides a rotameter, isoflurane scrubber, and anesthesia gas flow system similar to the Pearl Imager when an accessory imaging bed is locked in place on the docking station. This chapter provides information on assembly and operation of the Pearl Docking Station only. The remaining chapters provide specific operational details on each of the accessory imaging beds. Information on the standard Pearl Imaging Bed is not provided in this manual since it can be found in the Pearl Imager Operator's Manual. Page 1 Chapter 1: Pearl Docking Station Assembling the Pearl Docking Station WARNING: Isoflurane gas can leak in the amount supplied to the Pearl Imaging System Docking Station if the product is not used properly. Carefully follow the instructions in this chapter to ensure that the scavenging charcoal filter and user-supplied anesthesia system are properly connected, and that the nose cone on the imaging bed has a nose cone plug inserted when not in use. AVERTISSEMENT: L'anesthésie d'isoflurane peut fuir dans la quantité fournie à la station d'amarrage de Pearl si le produit n'est pas employé correctement. Suivez soigneusement les instructions dans ce chapitre pour vous assurer que le port d'échappement est relié à un filtre à charbon, que le système d'anesthésie fourni par l'utilisateur est correctement relié, et que le cone de nez est équipé d'un bouchon inséré. 1) Unpack the Pearl® Imaging System Docking Station and any other accessory imaging beds, such as the Pearl Clean Box, so all parts are available for assembly. 2) Loosen the thumb nuts on the back of the rotameter stand. Orient the rotameter with tubing to the back of the stand as shown below. Connect the rotameter to the rotameter stand by sliding the rotameter over the mounting studs (similar to the Pearl Imaging System instrument) and tight- ening the thumb nuts. Figure 1-2. Docking station rotameter connects to the stand via two thumb nuts with the rotameter oriented to the front of the stand. Page 2 Pearl® Imager Accessories 3) Connect the tube from the port labeled “OUT” on the rotameter to the port labeled “IN” on the back of the docking station. The gender of the connector prevents connection to the wrong port. Figure 1-3. Docking station back panel. 4) Connect the tube from the port labeled “EXHAUST” on the rotameter to the port labeled “OUT” on the back of the docking station. 5) If using the Pearl Clean Box, connect one of the provided HEPA filter assemblies (packaged with the Clean Box) to the port labeled “IN” on the rotameter. The gender of the connector assures the filter will be plugged-in in the correct orientation. Figure 1-4. Docking station rotameter with HEPA filter assembly installed between the IN port on the rotameter and the anesthesia gas supply tube from the vaporizer. Page 3 Chapter 1: Pearl Docking Station 6) Connect one of the provided HEPA filter assemblies between the SmartFlow system and the Induction Chamber. 7) Connect the opposite end of the HEPA filter assembly to the provided anesthesia gas supply tube and then connect the other end of the anesthesia gas supply tube to one of the output manifold connectors on the SmartFlow Anesthesia System, or equivalent (Figure 1-5). Note: For imaging beds not requiring a HEPA filter, connect the anesthesia gas supply tube dir- ectly to the rotameter. For other vaporizers, it may be necessary to remove the connector that con- nects to the SmartFlow System and replace it with an appropriate fitting. CAUTION: The Pearl® Imaging System Docking Station is compatible only with anesthesia systems that supply isoflurane gas. The flow rate and pressure into the instrument should not exceed 1 liter per minute and 1 psi, respectively. If the pressure exceeds 5 psi, the rotameter will not accurately show the flow to the nose cone. ATTENTION: La station d'amarrage de Pearl n'est compatible qu'avec les systèmes d'anesthésie qui fournissent le gaz isoflurane. Le débit et la pression dans l'instrument ne devraient pas excéder 1 litre par minute et 6.9 kPa, respectivement. Si la pression excède l'estimation de 34.5 kPa, le rotamètre ne montrera pas en juste proportion l'écoulement au cône de nez. EQUIPMENT LEGEND B C J E A OXYGEN TANK D B OXYGEN REGULATOR C SMARTFLOW ANESTHESIA SYSTEM M D SMARTFLOW VAPORIZER E INDUCTION CHAMBER I F PEARL IMAGER I N G PEARL ROTAMETER EX. H PEARL IMAGER IMAGING BED OR CLEAN BOX OUT K I CHARCOAL FILTER CANISTER A J HEPA FILTER ASSENBLY (9957-055) FOR 9930-022 PEARL CLEAN BOX F IN J K DOCKING STATION ROTAMETER AND STAND I L PEARL DOCKING STATION OUT IN M 10’ TUBING ASSEMBLY (#9957-056) INCLUDING EX. WITH THE PEARL DOCKING STATION OUT G N TUBING ASSEMBLY INCLUDDED WITH L THE PEARL IMAGER IN H J Figure 1-5.