Catalog # Aliquot Size U211-911-05 3 x 5 nmol U211-911-20 3 x 20 nmol U211-911-50 3 x 50 nmol UBE2B siRNA Set I siRNA duplexes targeted against three exon regions Catalog # U211-911 Lot # Z2109-16 Specificity Formulation UBE2B siRNAs are designed to specifically knock-down The siRNAs are supplied as a lyophilized powder and human UBE2B expression. shipped at room temperature. Product Description Reconstitution Protocol UBE2B siRNA is a pool of three individual synthetic siRNA Briefly centrifuge the tubes (maximum RCF 4,000g) to duplexes designed to knock-down human UBE2B mRNA collect lyophilized siRNA at the bottom of the tube. expression. Each siRNA is 19-25 bases in length. The gene Resuspend the siRNA in 50 µl of DEPC-treated water accession number is NM_003337. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot E2-17kDa; HHR6B; HR6B; RAD6B; UBC2 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or UBE2A Protein U210-30H below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2B Protein U211-30H µM can undergo up to 50 freeze-thaw cycles without UBE2C Protein U212-30H significant degradation. For long-term storage, it is UBE2D1 (UBCH5A) U213-30H recommended that the siRNA is stored at -70oC. For most Protein favorable performance, avoid repeated handling and UBE2D2 (UBC4) Protein U214-30H multiple freeze/thaw cycles. UBE2D3 (UBCH5C) U215-30H Protein Scientific Background UBE2B or ubiquitin-conjugating enzyme E2B is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair. UBE2B is UBE2B siRNA Set I specifically important during chronic low-dose ultraviolet siRNA duplexes targeted against three exon regions exposure to prevent counterproductive DNA checkpoint activation and allow cells to proliferate normally (1). The Catalog # U211-911 activity of UBE2B is required within the synaptonemal Lot # Z2109-16 complex and for meiotic recombination in spermatocytes Packaging Specifications 2.5 nmol/tube for 3 x 5nmol (2). Experimental inactivation of the UBE2B gene in mice Format Lyophilized powder causes male infertility. Stability 1yr at -70oC from date of shipment Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is References recommended that the lyophilized 1. Hishida, T. et.al: RAD6-RAD18-RAD5-pathway- and resuspended siRNAs are stored at dependent tolerance to chronic low-dose ultraviolet or below -20oC. After resuspension, light. Nature 457: 612-615, 2009. siRNA stock solutions ≥2 µM can undergo up to 50 freeze-thaw cycles 2. Baarends, W. M. et.al: Loss of HR6B ubiquitin- without significant degradation. For conjugating activity results in damaged long-term storage, it is recommended synaptonemal complex structure and increased that the siRNA is stored at -70oC. For most favorable performance, avoid crossing-over frequency during the male meiotic repeated handling and multiple prophase. Molec. Cell. Biol. 23: 1151-1162, 2003. freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .