Schedule of the XXII. Linz Winter Workshop 2020 Friday, Jan.31 19:00-23:00 Get Together & Registration Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Saturday, Feb.1 08:00-09:00 Registration Sommerhaus Hotel Julius-Raab-Heim, Ground Floor 09:00-09:05 Welcome / Opening Peter Hinterdorfer Johannes Kepler University Linz, Austria Hermann Gaub 09:05-09:50 Plenary Lecture Ludwig-Maximilian University Munich, Germany Force and function of proteins in-vitro and in-silico Session I: High-Speed AFM Chairman: Georg Fantner Preautophagosomal structure is formed by liquid-liquid phase Toshio Ando 09:50-10:15 1 separation through weak intermolecular interactions between Kanazawa University, Japan Atg13 and Atg17 Simon Scheuring High-speed atomic force microscopy: a forceful tool for 10:15-10:40 2 Weill Cornell Medicine, USA molecular biophysics Platinum sponsor talk Heiko Haschke From single molecule to medical applications – a new AFM 10:40-10:55 3 Bruker Nano GmbH, Germany toolkit for the nanoscopic investigation of mechanics, structures and dynamic processes in life science 10:55-11:15 Coffee Break & Exhibitions Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Session II: Advances in Chairman: Dario Anselmetti Scanning Probe Microscopy Direct cantilever actuation and multi-activation controls for Georg Fantner 4 11:15-11:40 multi-parametric high-speed AFM imaging EPF Lausanne, Switzerland Seeing is believing: probing protein oligomers and protein- Liming Ying 5 ligand interactions by single molecule spectroscopy and 11:40-11:55 Imperial College London, UK imaging Sebastian Wood 6 Quantitative nanoscale electrical and optical characterization 11:55-12:10 National Physical Laboratory, UK using atomic force microscopy Ulrich Schmid 7 12:10-12:25 High performance piezoelectric MEMS resonators in fluids Technical University Vienna, Austria 12:25-13:45 Lunch & Exhibitions Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Session III: Nanomicrobiology Chairman: Yoo Jin Oh Ulrike Endesfelder Visualizing the inner life of microbes by single-molecule 13:45-14:10 8 MPI for Terrestrial Microbiology, Germany localization microscopy Felipe Viela Mechanostability of the fibrinogen bridge between staphylo- 14:10-14:25 9 Université Catholique de Louvain, Belgium coccal surface protein ClfA and endothelial cell integrin V Mitchel Doktycz Capturing and correlation the molecular, physical and functional 14:25-14:40 10 Oak Ridge National Laboratory, USA characteristics of plant-associated microbes Molecular length and covalent anchorage of the lipoprotein Lpp Marion Mathelié-Guinlet 14:40-14:55 11 play key roles in regulating the mechanical properties of the Université Catholique de Louvain, Belgium Escherichia coli cell envelope Poster Session I 14:55-16:30 Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Coffee Break & Exhibitions Session IV: Cell Mechanics and Chairman: Gerhard Schütz Mechanobiology Gabriel Gomila 12 Mapping the nanoscale dielectric properties of cells by scanning 16:30-16:55 University of Barcelona, Spain dielectric force volume microscopy Ricardo Garcia Advances in single cell nanomechanics: high-resolution, sub- 16:55-17:20 13 CSIC Madrid, Spain surface imaging and nanorheology Marco Fritzsche 17:20-17:45 14 Nanoscale mechanobiology shapes T-cell activation University of Oxford, UK Fibronectin patches as anchoring points for force sensing and Thomas Schmidt 17:45-18:00 15 transmission in human induced pluripotent stem cell-derived Leiden University, The Netherlands pericytes Lukas Schrangl A FRET-based sensor for probing forces exerted by single T 18:00-18:15 16 Technical University Vienna, Austria cell receptors on their ligands 19:30 Meeting point Main Square Yellow trains depart for City Tour at 19:45 20:15-23:00 Conference Dinner LENTOS Art Museum Sunday, Feb. 2 Session V: Biomolecular Chairman: Hongbin Li Interactions Exploring the nature of catch bonds between cell surface Dario Anselmetti 09:00-09:25 17 sulfatases and heparin sulfate with AFM single molecule force Bielefeld University, Germany spectroscopy David Alsteens 09:25-09:50 18 Nanomechanical mapping of virus binding sites to animal cells Université Catholique de Louvain, Belgium Wesley Wong Probing biomolecular interactions with parallel force 09:50-10:15 19 Harvard Medical School, USA spectroscopy 10:15-10:40 Joon Won Park 20 Ultra-sensitive quantification of various DNA biomarkers with Pohang University, South Korea AFM 10:40-11:00 Coffee Break & Exhibitions Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Session VI: Single Molecule Chairman: Giovanni Dietler Mechanics Laura Finzi 11:00-11:25 21 The effects of tension on transcription Emory University, USA Hongbin Li Single molecule force spectroscopy reveals the mechanical 11:25-11:50 22 University of British Columbia, Canada design governing the efficient translocation of the bacterial toxin protein RTX Christian Kaiser Energetic dependencies dictate folding mechanism in a multi- 11:50-12:05 23 Johns Hopkins University, USA domain protein Damien Sluysmans 12:05-12:20 24 Probing the mechanochemical properties of a synthetic University of Liège, Belgium overhand knot by AFM Tamás Hegedűs 12:20-12:35 25 The folding pathway of the CFTR nucleotide binding domain 1 Semmelweis University, Hungary 12:35-13:45 Lunch & Exhibitions Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Session VII: Nanoscopy and Chairman: Peter Pohl Nanospectroscopy Stefan Jakobs 13:45-14:10 26 Focusing on mitochondria with super-resolution microscopy MPI for Biophysical Chemistry, Germany Alexander Jesacher 27 3D single molecule localization microscopy close to the 14:10-14:35 Medical University of Innsbruck, Austria coverslip: exploiting “forbidden light” for high precision measurements Steve Pressé New mathematics for new physics: learning about single 14:35-15:00 28 Arizona State University, USA molecules one photon at a time Poster Session II 15:00-16:30 Sommerhaus Hotel Julius-Raab-Heim, Ground Floor Coffee Break & Exhibitions Session VIII: Correlative Imaging Chairman: Mitchel Doktycz Rainer Hillenbrand Chemical nanoimaging by infrared nanoscopy and 16:30-16:55 CIC nanoGUNE and Ikerbasque, Spain 29 nanospectroscopy Multifrequency-AFM-IR platform for chemical and subsurface 16:55-17:10 Eric Lesniewska 30 element density analysis University of Bourgogne, France Patrick Frederix Platinum sponsor talk 17:10-17:25 31 Nanosurf AG, Switzerland CoreAFM with an integrated inverted microscope and FluidFM Gold sponsor talk Pablo Dörig 17:25-17:40 32 Applications of FluidFM hollow probes in biophysics and life Cytosurge AG, Switzerland sciences Petr Gorelkin Directly examining of cellular mechanics with scanning ion 17:40-17:55 33 NUST “MISiS”, Russia conductance microscopy (SICM) Conference Dinner Buses depart in front of the Sommerhaus Hotel at 18:45 18:30-23:00 in Cider Press House Freiseder Boarding starts at 18:30 Monday, Feb. 3 Session IX: Advanced Chairman: Matthias Rief Bioimaging Mervyn Miles Vertically-oriented-probe (VOP) force microscopy for bio imaging: 09:00-09:25 34 University of Bristol, UK new developments Pierre-Emmanuel Milhiet 35 09:25-09:40 Septin oligomerization induces membrane remodelling University of Montpellier, France George Heath CLC antiporter dimerization dynamics revealed by novel 09:40-09:55 36 University of Leeds, UK developments in high-speed AFM Jürgen Strasser 09:55-10:10 University of Applied Sciences Upper 37 The dynamics of IgG oligomerization on antigenic surfaces Austria, Austria Victor Gisbert High-speed bimodal AFM nanomechanical mapping of collagen 10:10-10:25 38 CSIC Madrid, Spain self-assembly 10:25-10:45 Coffee Break & Exhibitions Sommerhotel Julius-Raab-Heim, Ground Floor Session X: Single Molecule Chairman: Sandor Kasas Force Spectroscopy Matthias Rief 10:45-11:10 39 Single molecule mechanics of proteins Technical University, Germany Zhu Rong Studies of the interaction between desipramine and monoamine 11:10-11:25 40 Johannes Kepler University Linz, Austria transporters using force spectroscopy 11:25-11:40 Christina Lo Giudice 41 Human glucagon receptor is activated through a “dock and lock” Université Catholique de Louvain, Belgium mechanism Gurunath Apte HIT antibodies as mediators between platelets and breast 11:40-11:55 Institute for Bioprocessing and Analytical 42 cancer cells Measurement Techniques, Germany Alessandra Griffo On the nanoscale interactions of recombinant proteins toward 11:55-12:10 43 Saarland University, Germany bio-based hybrid materials: an AFM study Claudia Gusenbauer 12:10-12:25 44 Chemical mapping of lignocellulosic substrates via chemical University of Natural Resources and Life force microscopy Sciences, Tulln, Austria 12:25-13:45 Lunch & Exhibitions Sommerhotel Julius-Raab-Heim, Ground Floor Session XI: Cellular and Chairman: Mervyn Miles Membrane Mechanics Giovanni Dietler 13:45-14:10 45 AFM: new and old stuff under the tip EPF Lausanne, Switzerland Shivprasad Patil 14:10-14:25 Indian Institute of Science Education and 46 Differential stiffness of body column facilitates the somersault Research, India locomotion of hydra on solid substrates Yeraldinne Carrasco-Salas 14:25-14:40 47 AFM studies of the mechanical properties of AAC capsids at the University of Lyon, France single virus level Leda Lacaria 14:40-14:55 48 Mechanics and structure of the cortical cytoskeleton of University Aix-Marseille, France micropatterned cells Lorena Redondo-Morata In situ conversation of sphingomyelin to ceramide reveals a local 14:55-15:10 49 Institute Pasteur Lille, France increase of