CURRICULUM VITAE (Update: 4/1/21) PERSONAL Name: Kwang Poo Chang 张光樸 Address: Sex: Male Dept. Microbiology/Immunology Citizenship: Naturalized U.S. Center for Cancer Cell Biology, Immunology, Infection Dependents: Chin Shen (Wife, Biochemist) Chicago Med. Sch./Rosalind Franklin Univ. Med. & Sci. Patrick (Son, pediatrician) 3333 Green Bay Road, North Chicago, IL 60064, USA TEL 847-578-8837 FAX 847-578-3349 E-mail. [email protected] EDUCATION: B.Sc. 1965 Entomology/Plant Pathol, National Taiwan University, Taiwan M.Sc. 1968 Microbiology, University of Guelph, Ontario, Canada Ph.D. 1972 Cell Biology, University of Guelph, Ontario, Canada TEACHING/RESEARCH POSITION: Postdoctoral Fellow Oct. 1972 - Aug. 1974, Rockefeller University Research Associate Sept. 1974 - Feb. 1976, Rockefeller University Assistant Professor Mar. 1976 - Aug. 1979, Rockefeller University Associate Professor Sept. 1979 - Mar. 1983, Rockefeller University Professor (Tenured since 1984) April 1983 - Present, Chicago Medical School/UHS, FUHS, RFUMS TEACHING EXPERIENCE: University of Guelph (1967-1971): INSTRUCTOR (Biology/Entomology/Zoology) Rockefeller University (1972-1982): LECTURER (shared responsibilities: Parasitology/Seminar) Chicago Medical School/UHS (1983-2007): LECTURER (shared responsibilities: Medical Microbiology (Helminths, Protozoa, Entomology, Host-parasite Interactions); Molecular Biology (Molecular & Cell Biology Techniques); Molecular/Experimental Parasitology Chicago Medical School/RFUMS (2007-Present): LECTURER (shared responsibilities): Medical Microbiology (Ditto); Integrated Grad Program: (Molecular Techniques; 2007-present Molecular Cellular Biology II - RNA editing; Method-Immunotherapy/vaccination) National Medical School Review (1996) Medical Parasitology National Changhwa University, Taiwan (2011) GUEST LECTURER of Biotechnology Graduate School (Biological/Biomedical Research lectures) 12/17/11-01/04/12 Jinan Medical University, Guangzhou, PRC (2012) GUEST LECTURER School of Medicine (Med. Parasitology/Graduate School Seminars) 03/03-29/12 National Taiwan University, Taipei, Taiwan (2012) GUEST LECTURER Schools of Natural Science/Medicine (Short-course-Special Topics/Res. seminars) 09/23-10/09/12 RESEARCH INTEREST: CELL BIOLOGY/MOLECULAR BIOLOGY/BIOCHEMISTRY OF PARASITISM & SYMBIOSIS IMMUNOLOGY (Immunotherapy/vaccination) 2 KPChang CV (Con’t) AFFILIATED APPOINTMENTS: 1. CERTIFIED PROFESSOR – Nat Taiwan Univ Sch Med/Ministry of Education, Taipei, Taiwan 1989- 2. HONORARY PROFESSOR – Nat Inst Parasit Dis/Chinese Academy of Preventive Medicine, Beijing, 1996 3. VISITING PROFESSOR – Taipei Medical University, Taipei, Taiwan, 2013-2018 4. VISITING PROFESSOR – Laboratory of Parasitic Diseases, Xinjiang CDC, Urumqi, PR China, 2017- 5. VISITING PROFESSOR – Taipei Medical University, Taipei, Taiwan 11/1/2020-10/31/2023 HONORS/AWARDS: 1. PRESIDENT’S PRIZE for the best paper, Ann. Meet. Ent. Soc. Ontario, Canada, 1968 2. IRMA T. HIRSCHL CAREER SCIENTIST AWARD, New York City, 1977-1982 3. MORRIS E. PARKER AWARD Merit Sci Res, UHS/The Chicago Medical School, N Chicago, IL, 1985 4. SEYMOUR HUTNER AWARD, Society of Protozoologists, 1987 5. NIH INTERNATIONAL TRAVEL FELLOWSHIP - India-USA Science Exchange Program, Feb. 1992 6. VEL NAIR FELLOWSHIP – Rosalind Franklin University School of Graduate/Postgraduate Studies, 2005 UNIVERSITY COMMITTEE SERVICES: (University of Health Sciences=RFUMS/Chicago Medical School, 1983-present): Radiation Safety (1986-2007) Histopathology Facility (Ad hoc, 1986) Electron microscopy (1990) Bio-analytical Methods (Ad hoc, 1986) Animal Resources (1989-1999) Patent (Ad hoc, 1983) Faculty Award (1992-2016) Faculty Affairs (1988) Biohazard (2000-2014t) Pharmacology Chair Search (1997) Grad Sch Appointment (Chair) (2013-) University Senate (1986-1987) Miscellaneous departmental Committees. MEMBERSHIPS IN ACADEMIC SOCIETIES: American Society of Tropical Medicine & Hygiene 1977-2007 American Society of Microbiology 1976-1995; 2006-Present American Society of Parasitologists 1975-Present American Society for Cell Biology 1977-1993 American Federation of Tissue Culture Association 1979-1983 Royal Society of Tropical Medicine and Hygiene 1988-1990 Society of Protozoologists 1976-Present Society of Chinese Bioscientists in America 1986-present (Permanent member) Sigma Xi 1975-1977 New York Academy of Sciences 1975-1977 Parasitolgia Zoologica Academia Sinica 1987-Present 3 KPChang CV (Con’t) GRADUATE COURSES OFFERED: MB 5546 Research Techniques in Cell Biology (1983-2002) This course is to acquaint students in the Department with the following techniques: tissue and microbial culture, electron microscopy, protein chemistry, hybridoma, FACS, OPFAGE, DNA/RNA techniques. etc. Some or all listed, or other related techniques, are offered, depending on the number and interests of students. Students are encouraged to work on their own subject matters of interest. Fall quarter. One hr of lecture and 3 hrs of lab/wk. (K.-P. Chang and departmental faculty). MB 5547 Experimental Parasitology (1983-2005) Designed for students interested in basic research of parasitology, this course includes lectures, seminars and discussion with comprehensive coverage of the major tropical parasites currently under investigation. Students are to learn laboratory handling of Entamoeba, Leishmania, Plasmodium, Trypanosoma and other parasites as experimental models and their applications to resolve problems in tropical medicine and biology of parasitism in general. Spring quarter. Two hrs of lecture and three hrs of lab/wk. MB 5520 Molecular Parasitology (Since 1983 as needed) This course involves conceptual and practical aspects of applying new biotechnology to studying parasites and parasitic mechanisms of major tropical diseases. Emphasis is placed on biotechnology in handling proteins, DNA and RNA. Students are to actively participate in searching and discussing current literature as well as in project-oriented research. Research topics are catered to the background, training and/or interests of the students. Winter quarter. 3 hrs/wk lecture or discussion. Completion of a research project and submission of final report required. Shared responsibilities with other faculty members. MB5533 Departmental Seminar (1983-1987; 2006) Presentation of current research in Microbiology/Immunology by invited speakers, faculty and students. Required of all departmental students. Autumn, Winter and Spring quarters. One hr/wk. K.-P. Chang and faculty. MB 5532 Departmental Journal Club (2002-2006) Literature review by graduate students and faculty members. Articles selected by students and faculty members for evaluation and discussion. Students present and critique selected articles as well as serve as discussants. Autumn, Winter and Spring quarters. One hr/wk. K.-P. Chang and faculty. 4 KPChang CV (Con’t) RESEARCH OBJECTIVES AND CURRENT PROGRAMS: Our current supports and emphasis are to develop photo-inactivated Leishmania genetically and chemically as non-viable, but delivery competent carrier for immuno-therapy and -prophylaxis against leishmaniasis and other infectious/malignant diseases. My main research interest is to study molecular structures and biological functions of extrachromosomal self-replicating entities in eukaryotic cells. Biological models studied include parasitic protozoa in mammalian cells, endosymbiotic bacteria in insects and protozoa, and extrachromosomal circular DNA molecules amplified from chromosomes in response to drug selection. Emphasis is placed on the understanding of molecular integration and discordance of intracellular interactions. Experimental approach is multidisciplinary. My work supported by NIH has been focused on the Leishmania model for molecular mechanisms of microbial virulence. Specific infection-related molecules are invasins/evasins (invasive/evasive determinants) of the parasites, e. g. zinc protease/major surface glycoprotein (gp63), microsomal glycosyltransferase in the dolichol pathway of protein glycosylation and the ATP scavenging enzyme, e. g. nucleoside diphosphate kinases. Gp63 expression is regulated posttranslationally by N-glycosylation, which is in turn controlled by the glycosyltransferase gene expression. Secretory nucleoside diphosphate kinase is under study as an evasive determinant to prevent ATP-induced cytolysis and P2X7-mediated apoptosis of macrophages. My past and current research includes the following specific topics: (1) Molecular Biology: Structure, regulation and expression of virulence and virulence-regulating genes cloned from Leishmania DNA studied by molecular genetic approach, i. e. gene replacement and transfection; (2) Biochemistry: Purification and characterization of Leishmania gp63, microsomal N-acetylglucosamine-1-phosphate transferase and the ATP scavenging enzymes; (3) Cell Biology: Post-translational regulation of these molecules and their activities in intracellular parasitism of macrophages by Leishmania; and (4) Molecular immunology: Genetic analysis of immunologically active parasite epitopes as the pathogenic determinants responsible for the clinical symptoms (=virulence) manifested in human leishmaniasis. Prophylactic and diagnostic potentials of these epitopes targeted for preparing attenuated mutants as vaccines and recombinant products as antigens for diagnosis, prophylaxis and therapy; (5) Molecular epidemiology: Genetic/genomic polymorphism of Leishmania single-copy/multicopy genes in relation to their evolution and virulence in clinico-epidemiology. My