Product Datasheet NPVF Antibody GP-1080-50 Unit Size: 0.05 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Protocols, Publications, Related Products, Reviews, Research Tools and Images at: www.novusbio.com/GP-1080-50 Updated 2/5/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at www.novusbio.com/publications Submit a review at www.novusbio.com/reviews/destination/GP-1080-50 Page 1 of 3 v.20.1 Updated 2/5/2017 GP-1080-50 NPVF Antibody Product Information Unit Size 0.05 ml Concentration This product is unpurified. The exact concentration of antibody is not quantifiable. Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Clonality Polyclonal Preservative No Preservative Reconstitution Instructions Reconstitute with 50 ul sterilized water. Centrifuge to remove any insoluble material. Purity Unpurified Buffer Whole antisera Product Description Host Guinea Pig Gene ID 64111 Gene Symbol NPVF Species Sheep Reactivity Notes Sheep Specificity/Sensitivity Specificity was demonstrated by immunohistochemistry. Immunogen A synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response. Notes Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. Product Application Details Applications Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Immunohistochemistry, Immunohistochemistry-Paraffin 1:2000 Application Notes IHC. A concentration of 1 in 2000 is recommended for IHC. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. No staining is evident when the primary antibody is pre-absorbed with human and hamster RFRP-3. Page 2 of 3 v.20.1 Updated 2/5/2017 Images Flow Cytometry: NPVF Antibody [GP-1080-50] - Staining of RFRP-3 peptide is not affected by preadsorption with NFF peptide. Immunofluorescence: NPVF Antibody [GP-1080-50] - Immunoreactivity of cells and terminals in sheep hypothalamus section using anti-RFRP-3 antibody (1:2000). Immunofluorescence: NPVF Antibody [GP-1080-50] - Staining of RFRP- 3 peptide is not affected by preadsorption with Kisspeptin. Immunofluorescence: NPVF Antibody [GP-1080-50] - Staining of RFRP- 3 peptide is not affected by preadsorption with Prolactin-releasing peptide (PrRP). Novus Biologicals USA Novus Biologicals Canada 10730 E. Briarwood Avenue 461 North Service Road West, Unit B37 Centennial, CO 80112 Oakville, ON L6M 2V5 USA Canada Phone: 303.730.1950 Phone: 905.827.6400 Toll Free: 1.888.506.6887 Toll Free: 855.668.8722 Fax: 303.730.1966 Fax: 905.827.6402 [email protected] [email protected] Novus Biologicals Europe General Contact Information 19 Barton Lane www.novusbio.com Abingdon Science Park Technical Support: [email protected] Abingdon, OX14 3NB, United Kingdom Orders: [email protected] Phone: (44) (0) 1235 529449 General: [email protected] Free Phone: 0800 37 34 15 Fax: (44) (0) 1235 533420 [email protected] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit www.novusbio.com/guarantee Earn gift cards/discounts by submitting a review: www.novusbio.com/reviews/submit/GP-1080-50 Earn gift cards/discounts by submitting a publication using this product: www.novusbio.com/publications.