AR10151PU-S OriGene Technologies Inc. OriGene EU Acris Antibodies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-858-888-7900 Phone: +49-5221-34606-0 Fax: +1-858-888-7904 Fax: +49-5221-34606-11 [email protected] [email protected] Human Histidyl-tRNA synthetase / HARS - Purified Alternate names: HRS, HisRS, Histidine-tRNA ligase, JO1, Jo-1 antigen Catalog No.: AR10151PU-S Quantity: 10 µg Concentration: 1.2 mg/ml Background: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Uniprot ID: P12081 NCBI: NP_002100.2 GeneID: 3035 Species: Human Source: E. coli Format: State: Sterile Filtered clear solution. Purity: >90.0% as determined by both RP-HPLC and SDS-PAGE. Buffer System: 150mM NaCl and 10mM Sodium Phosphate, pH 7.2 Preservatives: 0.09% Sodium Azide Applications: Western Blot: Strongly reactive with Human anti Histidyl-tRNA Synthetase antisera. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Description: Histidyl-tRNA Synthetase Human Recombinant produced in E.coli is a single, non- glycosylated, polypeptide chain having a Molecular Mass of 55 kDa. The Histidyl-tRNA Synthetase is purified by proprietary chromatographic techniques. Molecular weight: 55 kDa Add. Information: Protein content: Protein quantitation was carried out by using 0.25-2.0 mg/ml Bra dford assay vs. BSA. Storage: Histidyl-tRNA Synthetase although stable at 2-8°C for 3 weeks, should be stored desiccated below -18°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. MS/20120827 Material Safety Datasheets are available at www.acris-antibodies.com or on request. Acris Antibodies is now part of the OriGene family. Learn more at www.origene.com 1 / 1 Powered by TCPDF (www.tcpdf.org).