Biological Safety Manual Prepared by: Environmental Health and Safety Office April 2012 Table of Contents Table of Contents .................................................................................................................... ii Tables and Figures ................................................................................................................. vii Acronyms ........................................................................................................................... viii Foreword ............................................................................................................................... x Document History .................................................................................................................... x 1.0 Introduction ........................................................................................................ 1-1 1.1 Biological Material ............................................................................................. 1-1 1.1.1 Biohazardous Material ........................................................................................ 1-1 1.1.2 Nonbiohazardous Material .................................................................................. 1-2 1.2 Regulations, Guidelines, and Permit Requirements ............................................. 1-2 1.3 Roles and Responsibilities ................................................................................... 1-4 1.3.1 Vice President for Research and Economic Development .................................... 1-4 1.3.2 Environmental Health and Safety Office (EHS) .................................................. 1-5 1.3.2.1 Laboratory Safety Personnel ............................................................................... 1-5 1.3.2.2 Occupational Health and Safety Personnel .......................................................... 1-5 1.3.3 Office of Sponsored Programs (OSP) .................................................................. 1-6 1.3.4 Office of Technology Transfer (OTT) ................................................................. 1-6 1.3.5 Office of Research Integrity and Assurance ......................................................... 1-6 1.3.6 Student Health Services ...................................................................................... 1-6 1.3.7 Physician or Other Licensed Healthcare Provider (PLHCP) ................................ 1-7 1.3.8 George Mason University Veterinarian ............................................................... 1-7 1.3.9 Waste Management Facility Operator ................................................................. 1-7 1.3.10 Institutional Biosafety Committee (IBC) ............................................................. 1-8 1.3.11 Institutional Animal Care and Use Committee (IACUC) ..................................... 1-8 1.3.12 Deans or Directors .............................................................................................. 1-9 1.3.13 Principal Investigator (PI)/Laboratory Supervisor (LS) ....................................... 1-9 1.3.14 Laboratory Personnel .......................................................................................... 1-9 1.3.14.1 Support Services Staff ....................................................................................... 1-10 2.0 Assessment and Management of Risk ................................................................. 2-1 2.1 Risk Assessment ................................................................................................. 2-1 2.1.1 Risk Groups (RG) ............................................................................................... 2-2 2.1.2 Laboratory Exposure ........................................................................................... 2-3 2.1.3 Biological Laboratory Hazards ............................................................................ 2-4 2.1.3.1 Infectious Material .............................................................................................. 2-4 2.1.3.2 Sharps ................................................................................................................. 2-5 2.1.3.3 Animal Allergens ................................................................................................ 2-5 2.1.3.4 Zoonoses ............................................................................................................. 2-5 2.1.3.5 Physical Hazards in Animal Spaces ..................................................................... 2-5 2.1.3.5.1 Ergonomic Hazards ............................................................................................. 2-5 2.1.3.5.2 Slips, Trips, and Falls .......................................................................................... 2-6 2.1.3.5.3 Electrical Hazards ............................................................................................... 2-6 2.1.3.5.4 Heat Stress .......................................................................................................... 2-6 2.1.3.5.5 Noise .................................................................................................................. 2-6 2.2 Risk Management ............................................................................................... 2-6 Environmental Health and Safety Office ii Biological Safety Manual 04/2012 2.2.1 Biosafety Levels ................................................................................................. 2-7 2.3 Samples of Unknown Hazard .............................................................................. 2-8 3.0 Engineering Controls .......................................................................................... 3-1 3.1 Facility Design .................................................................................................... 3-1 3.1.1 BSL-1 Facility Requirements .............................................................................. 3-1 3.1.2 BSL-2 Facility Requirements .............................................................................. 3-1 3.1.3 ABSL-1 Facility Requirements ........................................................................... 3-2 3.1.4 ABSL-2 Facility Requirements ........................................................................... 3-2 3.2 Safety Equipment ................................................................................................ 3-2 3.2.1 Emergency Showers and Eyewash Stations ......................................................... 3-3 3.2.2 First Aid and Spill Supplies ................................................................................. 3-3 3.2.3 Biosafety Cabinets .............................................................................................. 3-3 3.2.3.1 Class II Biosafety Cabinets ................................................................................. 3-4 3.2.3.2 Class III Biosafety Cabinets ................................................................................ 3-5 3.2.4 Negative Pressure Cage Rack .............................................................................. 3-5 3.2.5 Downdraft Necropsy Tables ................................................................................ 3-5 3.2.6 Vacuum Line Chemical Traps and Filters ............................................................ 3-5 3.3 Laboratory Equipment and Material .................................................................... 3-6 3.3.1 Safety Procedures for Centrifugation of Biohazardous Materials ......................... 3-6 3.3.2 Safety Procedures for Culture Plates, Tubes, and Bottles ..................................... 3-7 3.3.3 Safety Procedures for Use of Microtome or Cryostat ........................................... 3-8 3.3.4 Safety Procedures for Mechanical Disruption of Biohazardous Material.............. 3-8 3.3.5 Safety Procedures for Using Safety Blenders with Biohazardous Material ........... 3-9 3.3.6 Safety Procedures for Lyophilizing Biohazardous Material ................................. 3-9 3.3.7 Safety Considerations for Use of Miscellaneous Equipment with Biohazardous Material ........................................................................................ 3-9 3.4 Housekeeping ................................................................................................... 3-10 4.0 Administrative Controls ...................................................................................... 4-1 4.1 Training .............................................................................................................. 4-1 4.1.1 Biological Safety for BSL-2 Laboratories............................................................ 4-1 4.1.2 Bloodborne Pathogens ........................................................................................ 4-1 4.1.3 Autoclave Equipment Training............................................................................ 4-1 4.1.4 Animal and Vivarium Safety Training ................................................................. 4-1 4.1.5 Robinson Ventilation .......................................................................................... 4-2 4.1.6 BSL-3 Training ................................................................................................... 4-2 4.1.7 Shipping Class 6.2 Dangerous Goods, Biological Substances, and Dry Ice Training.................................................................................................