Title (en) Use of blocking-reagents for reducing unspecific T cell-activation Title (de) Verwendung von blockierenden Reagenzien zur Verringerung von unspezifischer T-Zellenaktivierung Title (fr) Utilisation de réactifs bloquants pour réduire l'activation de lymphocytes T non spécifiques Publication EP 3029067 A1 20160608 (EN) Application EP 14195645 A 20141201 Priority EP 14195645 A 20141201 Abstract (en) The present invention relates to a blocking-reagent for use in reducing unspecific T cell activation in T cell engaging therapies. The present invention further relates to pharmaceutical kit of parts and an in vitro method for evaluating unspecific T cell activation. IPC 8 full level C07K 16/24 (2006.01); A61K 39/395 (2006.01) CPC (source: EP US) A61K 35/17 (2013.01 - US); C07K 16/24 (2013.01 - EP); C07K 16/241 (2013.01 - US); C07K 16/2803 (2013.01 - US); C07K 16/2806 (2013.01 - US); C07K 16/2809 (2013.01 - US); C07K 16/2821 (2013.01 - US); C07K 16/2827 (2013.01 - US); C07K 16/2845 (2013.01 - US); C07K 16/2863 (2013.01 - US); C07K 16/2866 (2013.01 - US); C07K 16/2896 (2013.01 - US); C07K 16/3069 (2013.01 - US); G01N 33/56972 (2013.01 - US); A61K 2035/124 (2013.01 - US); C07K 2317/24 (2013.01 - US); C07K 2317/31 (2013.01 - US); C07K 2317/76 (2013.01 - EP US); C07K 2319/02 (2013.01 - US); G01N 2500/02 (2013.01 - US); G01N 2500/10 (2013.01 - US) Citation (applicant) • WO 2013092001 A1 20130627 - SYNIMMUNE GMBH [DE] • WO 9222653 A1 19921223 - GENENTECH INC [US] • US 5888508 A 19990330 - HILDRETH JAMES E [US] • US 5854070 A 19981229 - ROSE LYNN M [US] • EP 0057851 A2 19820818 - LEHMANN W MINOL HAUSTECH [DE] • WO 0170260 A1 20010927 - MILLENNIUM PHARM INC [US] • EP 2155783 B1 20130731 - AMGEN RES MUNICH GMBH [DE] • EP 2155788 B1 20120627 - MICROMET AG [DE] • WO 9954440 A1 19991028 - DOERKEN BERND [DE], et al • WO 2004106381 A1 20041209 - MICROMET AG [DE], et al • WO 2013092001 A1 20130627 - SYNIMMUNE GMBH [DE] • STAERZ UD; KANAGAWA 0; BEVAN MJ: "Hybrid antibodies can target sites for attack by T cells", NATURE, vol. 314, 1985, pages 628 - 631, XP008088110, DOI: doi:10.1038/314628a0 • PEREZ P; HOFFMAN RW; SHAW S; BLUESTONE JA; SEGAL DM: "Specific targeting of cytotoxic T cells by anti-T3 linked to anti-target cell antibody", NATURE, vol. 316, 1985, pages 354 - 356, XP001207655, DOI: doi:10.1038/316354a0 • JUNG G; HONSIK CJ; REISFELD RA; MÜLLER-EBERHARD HJ: "Activation of human peripheral blood mononuclear cells by anti-T3: Killing of tumor target cells coated with anti-target X anti-T3-conjugates", PROC NATL ACAD SCI USA, vol. 83, 1986, pages 4479 - 4483 • JUNG G; MÜLLER-EBERHARD HJ: "An in vitro model for tumor immunotherapy with antibody-heteroconjugates", IMMUNOL TODAY, vol. 9, 1988, pages 257 - 260, XP023938678, DOI: doi:10.1016/0167-5699(88)91304-7 • JUNG G; FREIMANN U; V.MARSCHALL Z; REISFELD RA; WILMANNS W: "Target cell induced T cell activation with bi- and trispecific antibody molecules", EUR J IMMUNOL, vol. 21, 1991, pages 2431 - 2435, XP000647636, DOI: doi:10.1002/eji.1830211020 • BARGOU R; LEO E; ZUGMAIER G ET AL.: "Tumor regression in cancer patients by very low doses of a T cell-engaging antibody", SCIENCE, vol. 321, 2008, pages 974 - 977 • ADAMS GP; WEINER LM: "Monoclonal antibody therapy of cancer", NAT BIOTECHNOL., vol. 23, 2005, pages 1147 - 57, XP002456710, DOI: doi:10.1038/nbt1137 • TOPP MS; KUFER P; GOKBUGET N ET AL.: "Targeted therapy with the T cell-engaging antibody blinatumomab of chemotherapy-refractory minimal residual disease in B-lineage acute lymphoblastic leukemia patients results in high response rate and prolonged leukemia-free survival", J CLIN ONCOL, vol. 29, 2011, pages 2493 - 2498, XP055112754, DOI: doi:10.1200/JCO.2010.32.7270 • KROESEN BJ; BUTER J; SLEIJFER DT ET AL.: "Phase I study of intravenously applied bispecific antibody in renal cell cancer patients receiving subcutaneous interleukin 2", BR J CANCER, vol. 70, 1994, pages 652 - 661 • TIBBEN JG; BOERMAN OC; MASSUGER LF ET AL.: "Pharmacokinetics, biodistribution and biological effects of intravenously administered bispecific monoclonal antibody OC/TR F(ab') in ovarian carcinoma patients", INT J CANCER, vol. 66, 1996, pages 477 - 483, XP001085380, DOI: doi:10.1002/(SICI)1097-0215(19960516)66:4<477::AID-IJC11>3.0.CO;2-5 • MAUS MV; GRUPP SA; PORTER DL; JUNE CH: "Antibody-modified T cells: CARs take the front seat for hematologic malignancies", BLOOD, vol. 123, 2014, pages 2625 - 35, XP055185132, DOI: doi:10.1182/blood-2013-11-492231 • MORGAN RA1; YANG JC; KITANO M; DUDLEY ME; LAURENCOT CM; ROSENBERG SA: "Case report of a serious adverse event following the administration of T cells transduced with a chimeric antigen receptor recognizing ERBB2", MOL THER, vol. 18, no. 4, 2010, pages 843 - 851, XP055023624, DOI: doi:10.1038/mt.2010.24 • ROELAND LAMERISA; RENEE C.G. 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