ABSTRACT BOOK VerMidi XX ENS Lyon Amphithéâtre Mérieux 2017 VERMIDI XX PROGRAM 9:30 - 10:00 Welcome Breakfast 10:00 - 10:45 Keynote Lecture: Thorsten Hoppe Ubiquitin sets the timer: coordination of aging and proteostasis 10:45 - 12:00 Session 1 10:45 - 11:05 Céline Jenzer (I2BC, Gif sur Yvette) Determination of functional specificity of autophagic proteins during phagocytosis of apoptotic cells in embryo. 11:05 - 11:25 Philippe Tardy (INMG, Lyon) ß-spectrin and ankyrin regulate K2P channel function and localisation in C. elegans 11:25 - 11:45 Georgia Rapti (Rockefeller University, New York) Glia and pioneer neurons initiate C. elegans brain assembly through non-canonical Chimaerin/Furin axon guidance 11:45 - 12:00 A word from our sponsors 12:00 - 14:30 Lunch / Poster Session 14:30 - 15:50 Session 2 14:30 - 14:50 Germano Cecere (Institut Pasteur, Paris) small RNAs in epigenetic inheritance 14:50 - 15:10 Denis Dupuy (IECB, Bordeaux) Quantitative meta analysis of RNAseq data reveals a stochastic component of alternative splicing and transplicing in C. elegans 15:10 - 15:30 Lise Frézal (IBENS, Paris) Evolutionary variation in the heat-triggered mortal germ line phenotype in C. elegans 15:30 - 15:50 Frances Edwards (IJM, Paris) BUB-1 is at the core of antagonistic activities in the establishment of kinetochore attachments to the mitotic spindle 15:50 - 16:20 Cofee break 16:20 - 17:30 Session 3 16:20 - 16:40 Rodrigo Caceres (Institut Curie, Paris) Role of acto-myosin cytoskeleton in cell invasion in Caenorhabditis elegans 16:40 - 17:00 Xinyi Yang (IBPS, Paris) Rotating and elongating embryos: SPIM microscopy reveals how C. elegans embryos extend through a ratchet mode 17:00 - 17:20 Grégoire Michaux (IGDR, Rennes) Coordinated morphogenesis through tension induced planar polarity 17:30 Happy Hour Amphitéâtre Charles Mérieux DIRECTIONS Amphithéâtre Mérieux Place de l'École, 69007 Lyon, France Métro B | Debourg ou Stade de Gerland Tram T1 | ENS Lyon Métro B <> Gare Part Dieu Métro B Amphithéâtre Debourg Mérieux Métro B Stade de Gerland SPONSORS by Hybrigenics Services The future of single-domain antibodies Discover our in vitro antibody selection from our synthetic library > Save time in selecting Abs without animal immunization > Select Abs recognizing native proteins & even specific conformations > Identify intrabodies i.e. functional inside cells > Generate Abs for non-immunogenic, conserved or unprocessed proteins > Use Abs for all applications: -Research (detection, quantification, co-crystallization, live cell imaging...), -Diagnosis (detection of micro-organisms...) -Therapy (agonists, antagonists…) Llama VHH advantageous properties VH - Single domain VL CH1 CL - Solubility > Better access to hidden epitopes CH2 - Small size VHH > Easy & rapid cloning steps and scalable Ab production in bacteria - Affinity CH3 Camelid VHH - Stability > Versatile use of multi-species Fc cassettes to generate fully-functional Abs - Easy production Mammal IG Receive the cDNA clones of your validated antibodies! > Unlimited Ab supply at your own lab > No batch to batch variability > Direct use in in vitro and in vivo applications Scan & Watch our video Enter a new age of antibodies with Hybrigenics Services www.hybribody.com [email protected] FR: +33 (0)1 58 10 38 29 US: +1 617-588-3272 Table of contents Determination of functional specificity of autophagic proteins during phagocytosis of apoptotic cells in embryo., C´eline Jenzer [et al.] ....................... 5 ß-spectrin and ankyrin regulate K2P channel function and localisation in C. elegans, Philippe Tardy [et al.] .......................................... 6 Glia and pioneer neurons initiate C. elegans brain assembly through non-canonical Chi- maerin/Furin axon guidance, Georgia Rapti [et al.] ..................... 7 small RNAs in epigenetic inheritance, Germano Cecere ................... 8 Quantitative meta analysis of RNAseq data reveals a stochastic component of alternative splicing and transplicing in C. elegans., Nicolas Tourasse [et al.] .............. 9 Evolutionary variation in the heat-triggered mortal germ line phenotype in C. elegans., Lise Fr´ezal[et al.] .......................................... 10 BUB-1 is at the core of antagonistic activities in the establishment of kinetochore at- tachments to the mitotic spindle., Frances Edwards [et al.] ................. 12 Role of acto-myosin cytoskeleton in cell invasion in Caenorhabditis elegans, Rodrigo C´aceres [et al.] ......................................... 13 Rotating and elongating embryos: SPIM microscopy reveals how C. elegans embryos extend through a ratchet mode, Xinyi Yang [et al.] ..................... 14 Coordinated morphogenesis through tension induced planar polarity, Ghislain Gillard [et al.] ................................................ 15 A spectrin/PAK/actin-capping network stabilizes cell shapes in a morphogenetic ratchet process during C. elegans elongation, Gabriella P´asti [et al.] ................ 16 CRLD-1A is a membrane-associated protein disulfide isomerase required for acetylcholine receptor biogenesis, Manuela D’Alessandro [et al.] ...................... 17 1 Mitochondria dynamics modulation and WAH-1 (AIF homolog) diminution reduce phys- iopathological muscle degeneration in C. elegans., Charlotte Scholtes [et al.] ....... 18 EPIDERMAL RESPONSE TO FUNGAL INFECTION AND WOUNDING IN C. EL- EGANS, Clara Ta↵oni [et al.] ................................. 20 Exploring fungal virulence using C. elegans, Xing Zhang [et al.] .............. 21 Channel Nucleoporins recruit the Polo-like kinase PLK-1 to Nuclear Pore Complexes in prophase to direct Nuclear Envelope Breakdown, Lisa Martino [et al.] .......... 22 Mechanisms of immune activation after cuticle damage, Jean-Christophe Lone [et al.] .. 23 Fonctional study of Katanin, a prototype of microtubule-severing enzyme essential for C.elegans meiosis, Nicolas Joly [et al.] ............................ 25 An Atypical Mechanism of Chromosome Segregation in the C. elegans Oocyte, Kimberley Laband [et al.] ......................................... 26 Deciphering the role of Notch signaling in Y to PDA transdi↵erentiation in vivo in c.elegans., Laura Vibert [et al.] ................................ 27 Actin cytoskeleton in cell invasion and migration in Caenorhabditis elegans vulval devel- opment, Nagagireesh Bojanala [et al.] ............................ 28 Cellular innovations at the origin of pseudogamy in nematodes, Manon Grosmaire [et al.] 30 Characterization of the SET1/MLL complexes in C.elegans, Flore Beurton [et al.] .... 31 Post-transcriptional histone modifications maintain cell identity and genome stability in the Caenorhabditis elegans germline, Marion Herbette [et al.] ............... 32 Asymmetric cell division and the cytoskeleton in nematodes, Majdouline Abou-Ghali [et al.] ................................................ 33 Analysis of syndecan function at the C. elegans neuromuscular junction., Camille Va- chon [et al.] ........................................... 34 Identification of novel regulators of GABAergic synaptogenesis in the nematode Caenorhab- ditis elegans., Marine Gueydan [et al.] ............................ 35 The role of sel-10 in transdi↵erentiation, C´ecile Delance [et al.] .............. 36 Analysis of activity-dependent synaptogenesis at the SAB neuromuscular junction., Alexis Weinreb [et al.] ......................................... 37 2 A Simple and rapid high throughput method to isolate large male populations., Aniela Zablocki [et al.] ......................................... 38 Combining optogenetic and transcriptomic approaches to dissect thermal nociceptor ha- bituation in C. elegans using LiTeSt: a versatile Light and Temperature Stimulation platform, Andrei-Stefan Lia .................................. 39 FRET imaging and thermalized microfluidics: a combined approach to monitor the C. elegans CaMK-CREB pathway in vivo, Gabriella Saro [et al.] ............... 40 Screening for nociception genes in C. elegans using an optogenetic approach, Filipe Marques 41 Identification of novel regulators of the two-pore domain potassium channel EGL-23 in Caenorhabditis elegans, Sonia El Mouridi [et al.] ...................... 42 wrmScarlet, seeing is believing., Sonia El Mouridi [et al.] .................. 44 COULD OUR LIFE BE SWEETER AND LONGER?, Alexia Gomez [et al.] ...... 45 List of participants 45 3 KEYNOTE LECTURE Ubiquitin sets the timer: coordination of aging and proteostasis Thorsten HOPPE University of Cologne Germany. 4 Determination of functional specificity of autophagic proteins during phagocytosis of apoptotic cells in embryo. 1 1 1 1 C´eline Jenzer ⇤ , Elena Simionato , C´eline Largeau , Renaud Legouis† 1 I2BC – Centre National de la Recherche Scientifique - CNRS – Avenue de La Terrasse, 91198 Gif sur Yvette, France Phagocytosis and autophagy are two lysosome-mediated processes involved in the clearance of extra- cellular and intracellular components, respectively. These two processes are involved in various human diseases such as cancers. Previously, the laboratory has shown a sequential and specific involvement of autophagic proteins in the autophagic cascade [1,2]. Recent studies have identified the recruitment of autophagic proteins during phagocytosis of apoptotic corpses in the so called LC3-associated phagocy- tosis (LAP) [3]. LAP is a distinct process from autophagy but it relies on some members of autophagy pathway to allow an efficient degradation of the phagocytosed cargo. The objective of this study is to