The 3rd International Symposium on Medicinal Plants, Their Cultivation and Aspects of Uses BeitZaman Hotel & Resort Petra - Jordan November 21-23/ 2012 AAbbssttrraacctt BBooookk Sponsors http://www.mohammadasfour.com المملكت اﻷردنيت الهاشميت رقم اﻹيداع لدي دائزة المكتبت الىطنيت )2012/10/4010( )ردمك( ISBN 978-9957-31-012-7 يتحمل المؤلف كامل المسؤوليت القانىنيت عن محتىي مصنفه و ﻻ يعبز هذا المصنف عن رأي دائزة المكتبت الىطنيت أو أي جهت حكىميت أخزي THE 3rd INTERNATIONAL SYMPOSIUM ON MEDICINAL PLANTS, THEIR CULTIVATION AND ASPECTS OF USES BeitZaman Hotel & Resort Petra - Jordan November 21-23/ 2012 AAbbssttrraacctt BBooookk Chairman: Dr. Mohammad Abu Darwish Al-Balqa' Applied University Chief in Editor: Dr. Mohammad Abu Darwish Al-Balqa' Applied University Editors: Ziad H.M. Abu-Dieyeh Dr. Ezz Al-Dein Al-Ramamneh المحررون : : د. محمـد سـند أبو درويش م. زياد حمدان محمود أبو دية د. عز الدين محمـد الرمامنة Welcome Dear Participants, It is a great pleasure to welcome you on my own behalf, and on behalf of steering, and scientific committees of The 3rdInternational Symposium on Medicinal Plants, Their Cultivation and Aspects of Uses, as you are meeting here in the Red–Rose city of Petra; which is famous with its history and civilization. A city that was a commercial as well as a cultural center where caravans met to continue their ways from east to west. Today, we are here again for the third time, to meet these elite scientists and researchers, from different countries of the world. They came from famous universities, institutes, and research centers to present their result's researches in an old-renewable science (Plant Science). A science in which agriculture, pharmacy, medicine, and biology have interfered. By this, we confirm that humans and humanity are linked to the nature, craving to return to it, conserving it too. We also confirm that our scientific research will continue, deeping into the knowledge of medicinal plants -been cultivated, harvested, used, and studied in the laboratory- until being manufactured to various forms. It will be away to modernize the originality, and to originate the modernity. This conference will deal with your various, scientific researches in medicinal plants in the following main topics: Using medicinal plants and their extracts in the pharmacological activities, Ethno-pharmacological studies, Using medicinal plants as food additives in food recipes, Quality control and standardization of its products, Their conventional and organic cultivation and Impact of cultivation on their biodiversity, Climate change and ethno-botanical sources, Ethno-veterinary and animal nutrition practices of medicinal plants, Using of their extracts as parts of integrated pest management programs, New approaches in bio-fuel production and their industrial utilization. Outstanding eleven Keynotes speakers, who came from prestigious scientific institutes, will talk to us in order to enrich our conference with researches through their long–year's scientific research. This conference will create a great opportunity to communicate between participants; it is also a chance to exchange ideas and experiences, however, this chance will ease their future communication in order to hold new prospects of scientific co-operation. i This conference is a result of your participation, and the effort of members of steering, scientific, and executive committees. Not only this, it is also a result of partnership with public and private Jordanian as well as foreign institutions that contributed to success of the conference, like: Scientific Research Support Fund, Petra Development and Tourism Region Authority, The Housing Bank for Trade & Finance, DELASS for Natural Products, Jordan River Foundation, Aqaba International Laboratories (Ben Hayyan), VAPCO Company for Veterinary Medicines, Baraka Farms, Arab Federation of MAP, Pharmacognosy Communications, Ministry of Agriculture, Jordan TV, Ro'ya TV, Nature Middle East and Asfour Company. All thanks and appreciation is extended for you and them. I would like to extend my thanks to the Presidency of Al- Balqa Applied University represented by its president Professor Nabil Shawagfeh for supporting this conference. Once again, I welcome you in the Hashemite Kingdom of Jordan. A country full of love, goodness and peace. We wish you all a fruitful meeting. Dr. Mohammad Sanad Abu Darwish Al-Balqa' Applied University Chairman of the Symposium ii SCIENTIFIC COMMITTEE - Mohammad Sanad Abu-Darwish (Chairman)/Al-Balqa' Applied University, Jordan - Alain Meybeck / AM Phyto-Conseil, France - Geoffrey A. Cordell / Natural Products Inc.,Evanston.IL.USA - Carlos Garbisu / Neiker-Tecnalia,Spain - Hao Feng/University of Illinois at Urbana-Champaign.USA - Caroline Weckerle / University of Zurich, Switzerland - Ivan Salamon/Presov University, Slovakia - Judit Hohmann /University of Szeged,Hungary - Kyslychenko V.C. / National University of Pharmacy, Ukraine - David Draper Munt / Universidad Politecnica de Madrid,Spain - Lígia Salgueiro/University de Coimbra, Portugal - Fatma Afifi /Faculty of Pharmacy, University of Jordan,Jordan - Mohammed Hmamouchi / AFAMP, Morocco - Khulood Al Samararrae/ Al Nahrain University,Iraq - Rudolf Bauer / University of Graz, Austria - Nasri Haddad /ICARDA,Jordan - Wade Yang/University of Florida, USA - Mohamed Rashwan Abdel-Aal/ Assiut University ,Egypt. - Talal Ahmad Aburjai / Faculty of Pharmacy, University of Jordan , Jordan - Abu-Dieyeh, Ziad H. M. / Al-Balqa' Applied University, Jordan - Bochra Laribi /National Agronomic Institute of Tunisia, Tunisia - Abdelrahman Tawaha /Al-Hussein Bin Talal University, Jordan - Bayan Abdulhaq / Amman Arab University , Jordan - Ezz Al-Dein Al-Ramamneh /Al-Balqa' Applied University, Jordan - Faisal Awawdah / NCARE, Jordan - Hashem Taha / Delass Natural Products, Jordan - Kamal Khairallh/ Freelancer, Jordan - Majed Hasanat / PDTRA, Jordan - Mohammad Shahbaz / NCRD & IUCN, Jordan - Taha M. Rababah/Jordan University of Science&Technology,Jordan - Nemer Hadadin / Ministry of Agriculture, Jordan - Oraib Nawash/ Royal Botanic Garden, Jordan - Tariq Abo-Taleb/ Royal Botanic Garden, Jordan - Muhammad H. Aludatt / JUST, Jordan - Anwar Elhalah / Jordan River Foundation, JRF, Jordan - Sobhia Saifan/ NCARE, Jordan LOCAL ORGANIZING COMMITTEE Honorary Chairman: Prof. Dr. Nabil Shawagfeh Mohammad Sanad Abu-Darwish (Chairman) Abu-Dieyeh, Ziad H.M. AbedAlrzaq Shqirate Ahmad Al-Fraihat Ashraf Al-Shaer Eitaf Rawashdeh Ezz Al-Dein Al-Ramamneh Khaled Al-Habahbeh Odeh Murad Omar Al-Bdoor Saddam Al-Dalain Sati Al-Dalain Talib Al-Mseidein Ziad Al-Rawashdeh iii EXCUTIVE COMMITTEE Chairman: Ziad H. M. Abu-Dieyeh AbedAlrzaq Shqirate Adel Marahleh Afaf Rafaye'ah Ahmad Abbas Ahmad Bdawi Rafaye'ah Ali Tawara Aman Awadat Bader Tawara Doa'a Habahbeh Ekhlas Dhayat Ezdehar Shu'aibat Fawaz Rafaye'ah Hassaan Osofi Haya Rafaye'ah Heyam Shqirate Khaled Habahbeh Khaleel Bdour Majed Hasanat Majeda Aghawani Marwan Habahbeh Mohammad A. Bdour Mohammad A. Rafaye'ah Mohammad H. Bdour Mohammad Khliefat Mohammad R. Shqirate Mohammad T. Bdour Moneer Shqirate Nedal Bdour Nora Hwartheh Odeh Murad Rakan Nasarat Reem Khoshman Saleh Bdour Sohilah AboDarweesh Somya Shkhibi Tahani Bdour Talib Mseidein TECHNICAL SECRETARIAT Ekhlas Dhayat , Ezdihar Al-Shu'aibat , Tahani Bdour iv INVITED SPEAKERS Prof. Dr. Alain Meybeck / AM Phyto-Conseil, France Prof. Dr. Fatma Afifi / University of Jordan, Jordan Prof. Dr. Judit Hohmann / University of Szeged, Hungary Prof. Dr. Jacobus Nicolaas Eloff / University Pretoria Prof. Dr. Inteaz Alli / McGill University, Canada Prof. Dr. Lyoussi Badiaa / University of Fez, Morocco Dr. Matthias Lorenz / Phyto consult, Germany Prof. Dr. Mohammed Hmamouchi / Arab Federation of MAP, Morocco Prof. Dr. Rudolf Bauer / University of Graz, Austria Dr. Caroline Weckerle / University of Zurich Prof. Dr. Viljoen A. M. / Tshwane University of Technology, South Africa v The 3rd International Symposium on Medicinal Plants, their Cultivation and Aspects of Uses Topic (1): Pharmacological Activities of Medicinal Plants and Their Extracts 1.1 Evaluation of the Volatile Oil Composition and Antiproliferative Activity of Laurus nobilis L. (Lauraceae) on Breast Cancer Cell Line Models Abu-Dahab, R., Kasabri, V., and Afifi, F. Faculty of Pharmacy, University of Jordan, Amman, Jordan. Abstract: Volatile oil composition and antiproliferative activity of Laurus nobilis fruits and leaves grown in Jordan were under investigation. Volatile oil obtained by hydro-distillation was identified by GC-MS. Crude ethanol and other solvent extracts were prepared and antiproliferative activity was assayed using sulphorhodamine B assay against breast cancer cell models (MCF7 and T47D). Periodontal fibroblasts were used as a model for healthy cells. GC-MS analysis of the essential oil of the fruits resulted in the identification of forty five components representing 99.17 % of the total oil content while the leaf essential oil yielded 37 compounds representing 93.1% of the total oil content. Oxygenated monoterpene 1,8 cineol was the main component in the fruit (29.82%) and leaf (36.83%) oils. The ethanol extract of the fruits exhibited prominent antiproliferative activity with an IC50 of 12.32 µg/ml for T47D cell line and 27.96 µg/ml for MCF7 cells, while the IC50 values of the leaves were higher (48.20 µg/ml for MCF7 and 19.77 µg/ml for T47D cells). Least activity was observed with the oils. In an attempt to study the safety of these extracts, the antiproliferative activity of the crude ethanol extract was