Table: PTMScan® Results, Phospho-PKA Substrate Motif (RRXS*/T*), label-free quantitation Study design: rat cell line PC-12; Trypsin digest; Antibody: Phospho-PKA Substrate Motif (RRXS*/T*), PTMScan® Kit #1984, #5565 Treatments: Untreated, forskolin treated Fold-Change Index (Forskolin/Untreated) Protein Name Phosphorylation Site Description Peptide Accession kD Count in Study Intensity (Untreated) 3 Adaptor/scaffold 4 10.4 AHNAK 159 PREDICTED: similar to AHNAK nucleoprotein isoform 1 isoform 1 RVtAYTVDVTGR XP_574618 581 2 8.69E+05 5 11.2 AHNAK 161 PREDICTED: similar to AHNAK nucleoprotein isoform 1 isoform 1 RVTAyTVDVTGREGVKDIDISSPE XP_574618 581 1 6.14E+04 6 2.8 AKAP1 101 A-kinase anchor protein 1 RRsESSGNLPSIVDTR O88884 92 1 2.49E+06 7 2.2 AKAP2 850 A kinase (PRKA) anchor protein 2 RKsALALRWE Q5U301 96 1 5.57E+05 8 6.7 AP3D1 754, 758, 760 adaptor-related protein complex 3, delta 1 subunit RHsSLPtEsDEDIAPAQRVDIITE XP_234908 136 1 5.47E+04 9 3.2 AP3D1 754, 760 adaptor-related protein complex 3, delta 1 subunit RHsSLPTEsDEDIAPAQRVDIITE XP_234908 136 1 3.61E+05 10 -6.2 CTNND1 320 catenin (cadherin associated protein), delta 1 LRsYEDM#IGEEVPPDQYYWAPLAQHE XP_001064294 105 1 5.76E+05 11 3.8 DOCK1 1756 dedicator of cyto-kinesis 1 RFsVSPASPCSQPTPPPVTPR NP_001137330 215 1 1.01E+06 12 1.7 FNBP1L 488 formin binding protein 1-like RHsSDINHLVTQGRESPE Q2HWF0 70 1 3.98E+05 13 -1.2 FRS2 132, 135, 138 fibroblast growth factor receptor substrate 2 LEVPRtPRtPTtPGLGAQSLPNGYPR EDM16633 57 2 2.59E+05 14 2.2 FRS2 428 fibroblast growth factor receptor substrate 2 DCTPTVFNFDIRRPsLE EDM16633 57 1 8.14E+05 15 3.2 IRS-2 557 PREDICTED: similar to Insulin receptor substrate 2 (IRS-2) (4PS) RVsGDGAQDLDR O70360 137 1 1.75E+05 16 2.4 MPDZ 1802 multiple PDZ domain protein IKAAPFHSERRPsQSSQVSE O55164 219 1 2.14E+06 17 1.4 MPDZ 1802, 1804, 1808 multiple PDZ domain protein IKAAPFHSERRPsQsSQVsE O55164 219 1 5.29E+05 18 2.3 MPDZ 1804, 1805 multiple PDZ domain protein AAPFHSERRPSQssQVSE O55164 219 1 5.37E+05 19 4.5 MTSS1 307 metastasis suppressor 1 KSsVCSSLNSVNSSDSR NP_001124035 86 1 2.74E+05 20 9.5 Nck1 21 NCK adaptor protein 1 RKPsVPDTASPADDSFVDPGER XP_217246 36 3 2.51E+05 21 2.1 PARD3 715 partitioning-defective protein 3 homolog RIsHSLYSGIE Q9Z340 149 1 3.76E+05 22 2.2 LIM 228 PDZ and LIM domain 5 RGsQGDIKQQNGPPR Q62920 63 4 2.01E+06 23 3.4 tensin 3 648 PREDICTED: similar to tensin-like SH2 domain containing 1 KPsAPTPTQAYGQSNYSTQTWVR XP_341257 117 1 1.16E+05 26 23.6 14-3-3 zeta 58 tyr 3-monooxygenase/tryp 5-monooxygenase activation prot, zeta polypep RSsWRVVSSIEQKTE P63102 28 2 2.02E+05 27 Adhesion or extracellular matrix protein 28 2.5 CTNNB1 552 beta-catenin RTsMGGTQQQFVE Q9WU82 85 2 2.91E+06 29 1.1 desmoplakin 174 PREDICTED: similar to desmoplakin isoform II isoform 1 RAsSKGGYTCQSGSGWDE Q4QQR7 332 2 5.62E+06 30 -2.6 desmoplakin 174, 175 PREDICTED: similar to desmoplakin isoform II isoform 1 RAssKGGYTCQSGSGWDE Q4QQR7 332 1 2.60E+05 31 1.2 desmoplakin 175 PREDICTED: similar to desmoplakin isoform II isoform 1 VRRASsKGGYTCQSGSGWDE Q4QQR7 332 1 4.87E+06 32 1.8 desmoplakin 56 PREDICTED: similar to desmoplakin isoform II isoform 1 RCtVNDQNSDGYCQTGTM#SR Q4QQR7 332 1 1.18E+05 33 -2.3 LAD1 62 ladinin SVERLPsVEEAE NP_001101412 57 1 1.17E+06 34 -1.4 Nogo iso2; Nogo 107; 107 Reticulon-4 GPLPAAPPAAPERQPsWE Q9JK11-2; Q9JK11 39; 126 2 1.92E+07 35 10.7 plakophilin 3 159 plakophilin 3 RLsSAHNGGSAFGAVGYGGAQPTPPM#PTRPVSFHE NP_001099785 90 4 6.07E+05 36 1.2 plakophilin 3 159, 188 plakophilin 3 RLsSAHNGGSAFGAVGYGGAQPTPPM#PTRPVsFHE NP_001099785 90 1 5.02E+05 37 Apoptosis 38 9.9 EI24 46 etoposide induced 2.4 mRNA RAsSLLAQR Q4KM77 39 1 1.31E+05 39 1.3 PAR-4 155 PRKC, apoptosis, WT1, regulator RStGVVNIPAAECLDEYEDDE Q62627 36 1 6.74E+05 40 -2.7 PEA-15 116 phosphoprotein enriched in astrocytes 15A YKDIIRQPsEEE Q5U318 15 1 1.54E+06 41 Cell cycle regulation 42 3.7 AFAP1L2 341 PREDICTED: similar to Protein KIAA1914 KKsTSLEPPDRSLE XP_217643 92 1 4.05E+05 43 3.4 CLASP1 600 rCG37592 SRsDIDVNAAASAK EDL87905 167 2 1.02E+06 44 6.0 CLASP1 658 rCG37593 TRRQsSGSTTNVASTSSDSR EDL87905 167 5 2.93E+05 45 6.0 CLASP1 658, 661 rCG37594 RQsSGsTTNVASTSSDSR EDL87905 167 1 8.97E+04 46 2.4 CLASP1 690 rCG37595 sSSPGKLLGSGYGGLAGGSSRGPPVTPSSE EDL87905 167 1 9.95E+05 47 3.9 CLASP1 692 rCG37596 SSsPGKLLGSGYGGLAGGSSR EDL87905 167 1 2.38E+05 48 7.7 B99 501 G-2 and S-phase expressed 1 RLsGLTPQGLPSSM#R XP_217016 80 2 1.78E+05 49 1.6 KAB1 968 rCG20491, isoform CRA_b IRQPsIDLTDDDQTSSVPHSAISDIMSSDQE EDL94780 170 1 2.35E+05 50 -1.1 NuMA-1 337 PREDICTED: similar to nuclear mitotic apparatus protein 1 isoform 1 RRtTQIINITMTK Q4G051 71 1 4.21E+05 51 5.0 NUSAP1 113 nucleolar and spindle associated protein 1 RRDsTTFQNQE NP_001101232 43 3 9.67E+05 52 2.5 NUSAP1 115 nucleolar and spindle associated protein 1 M#KRRDSTtFQNQE NP_001101232 43 1 4.79E+04 53 2.4 MCC 354 PREDICTED: mutated in colorectal cancers KKsSCSLSVAE XP_225962 112 1 4.00E+05 54 2.6 MCC 354, 355 PREDICTED: mutated in colorectal cancers KKssCSLSVAE XP_225962 112 1 6.54E+05 55 3.9 SPECC1L 833 SPECC1-like RSsTSSEPTPTVK Q2KN99 124 3 3.30E+06 56 14.1 SPECC1L 833, 836 SPECC1-like RSsTSsEPTPTVK Q2KN99 124 1 4.98E+04 1 LEGEND: * = phosphorylation; # = oxidized methionine, fold changes greater than 5 upon drug treatment are highlighted Table: PTMScan® Results, Phospho-PKA Substrate Motif (RRXS*/T*), label-free quantitation Study design: rat cell line PC-12; Trypsin digest; Antibody: Phospho-PKA Substrate Motif (RRXS*/T*), PTMScan® Kit #1984, #5565 Treatments: Untreated, forskolin treated Fold-Change Index (Forskolin/Untreated) Protein Name Phosphorylation Site Description Peptide Accession kD Count in Study Intensity (Untreated) 57 Chaperone 58 12.1 HSP20 16 heat shock protein, alpha-crystallin-related, B6 RAsAPLPGFSTPGR P97541 18 1 3.86E+05 59 Chromatin, DNA-binding, DNA repair or DNA replication protein 60 1.9 capicua 1394, 1405 capicua homolog KNsTDLDSAPEDPtSPKRK NP_001100960 164 2 1.06E+06 61 45.7 H1C 36 histone cluster 1, H1d KAsGPPVSE P15865 22 9 1.08E+05 62 -1.7 LBR 101 lamin B receptor SVsASYQADAKEKE O08984 71 1 2.70E+05 63 2.9 polybromo 1 39 PREDICTED: similar to polybromo 1 isoform 2 isoform 1 RLsSLPTVDPIAVCHE XP_001059112 181 1 9.24E+05 64 4.0 TERF2IP 43 telomeric repeat binding factor 2, interacting protein RLsTLILHGGGILCR Q5EAN7 43 1 4.59E+05 65 5.8 TTF1 103 PREDICTED: similar to transcription termination factor 1 RREsQTPAQENLE XP_001079280 96 1 8.21E+04 66 Cytoskeletal protein 67 51.5 cordon-bleu 1042 cordon-bleu homolog KVsSLASEE NP_001100706 122 1 5.88E+04 68 -1.6 cordon-bleu 219 cordon-bleu homolog KSsLGNDETDKE NP_001100706 122 2 1.02E+04 69 3.3 cordon-bleu 648 cordon-bleu homolog RNsMEKYLNGPAE NP_001100706 122 1 4.21E+05 70 7.9 DAL-1 423 erythrocyte protein band 4.1-like 3 RAsALIDRPAPYFE Q9JMB3 122 1 2.41E+05 71 1.9 EPPK1 2290, 2709, 3224 PREDICTED: similar to Epiplakin AQAATGFIIDPVHNRRLsVEE XP_001059215 370 1 6.03E+05 72 6.4 FLNB 2107 filamin, beta TSRAPsVATVGSICD XP_224561 278 1 2.27E+05 73 1.5 FNBP1 496 formin binding protein 1 RQsGLYDGQTHQTVTNCAQDRE Q8R511 71 2 1.65E+05 74 3.7 KIF23 903 kinesin family member 23 RsSTLAPAQPDGTESE NP_001101625 109 1 3.07E+05 75 1.1 KIF23 903, 904 kinesin family member 23 KRRssTLAPAQPDGTESE NP_001101625 109 1 4.28E+05 76 4.7 KIF23 904 kinesin family member 23 RSsTLAPAQPDGTESEWTDVE NP_001101625 109 2 3.80E+05 77 5.7 KIF23 905 kinesin family member 23 RSStLAPAQPDGTE NP_001101625 109 2 1.32E+06 78 -7.6 K14 40 keratin 14 M#SSVLAGGSCRAPsTYGGM#SR Q6IFV1 53 2 1.03E+06 79 -2.8 K14 42 keratin 14 M#SSVLAGGSCRAPSTyGGMSR Q6IFV1 53 1 7.33E+05 80 -1.9 eplin 225 PREDICTED: similar to Epithelial protein lost in neoplasm (mEPLIN) RLsENSCSLDDLE Q6J4T4 84 2 1.83E+06 81 -1.1 nestin; nestin iso2 1166; 1078 nestin RKsIDTQEPLWSTE P21263; P21263-2 209; 199 1 9.61E+06 82 -1.6 plectin 1 4641, 4645 plectin 1 AGsRRGsFDATGSGFSM#TFSSSSYSSSGYGR P30427 534 1 3.22E+05 83 -3.0 plectin 1 4645 plectin 1 RGsFDATGSGFSM#TFSSSSYSSSGYGR P30427 534 2 2.12E+06 84 1.3 RIL 119 reversion induced LIM RSsISGISLE P36202 36 1 2.02E+07 85 71.4 KIF13B 1718, 1719 kinesin 13B RRssGLQPQGAPE Q70AM4 197 1 9.17E+04 86 Enzyme, misc. 87 3.7 CPOX 117 coproporphyrinogen oxidase RCsTFM#SSPVTE Q3B7D0 49 1 1.25E+05 88 2.9 GYS1 709 glycogen synthase 1 (muscle) sNSVDTGPSSSLSTPTEPLSPTSSLGEERN A2RRU1 84 1 3.79E+05 89 2.4 HELZ 1785 helicase with zinc finger domain RIsSGAAQPCVEE NP_001099318 220 1 4.35E+06 90 3.4 HMGCS1 471 hydroxymethylglutaryl-CoA synthase 1 TYARRPsTNDHSLDE P17425 57 2 3.55E+06 91 3.5 UQCRC1 214 ubiquinol-cytochrome c reductase core protein I RLSRtDLTDYLSR Q68FY0 53 1 1.45E+06 92 G protein or regulator 93 -1.0 ARF GAP1 360 ADP-ribosylation factor GTPase activating protein 1 RSsDSWDIWGSGSASNNKNSNSDGWE Q62848 45 1 6.01E+05 94 7.4 ARHGAP17 710 nadrin RCsSSLPPIQAPNHPPPQPPTQPR Q99N37 94 1 6.31E+05 95 4.7 ARHGAP17 712 nadrin RCSSsLPPIQAPNHPPPQPPTQPR Q99N37 94 1 7.61E+05 96 4.0 ARHGAP23 790 PREDICTED: similar to Rho GTPase activating protein 21 HYsQDCSSIKAGR XP_220918 177 1 1.13E+05 97 -1.1 ARHGEF5 1079 PREDICTED: similar to rho guanine nucleotide exchange factor 5 isoform 1 RCsKLINSSQLLYQE XP_342677 177 1 5.87E+05 98 6.4 ARPP-19 104 cyclic AMP phosphoprotein VTGDHIPTPQDLPQRKPsLVASKLAG Q712U5 12 10 8.48E+06 99 8.5 DOCK6 41 dedicator of cytokinesis 6 RSSsSLGVPLTE NP_001102467 202 1 8.55E+05 100 1.1 RalGAPA1 753 PREDICTED: similar to GTPase activating RANGAP domain-like 1 isoform 3 SIVRQKtVDIDDSQILPR O55007 234 1 2.95E+05 101 -1.4 RalGAPA1 772 PREDICTED: similar to GTPase activating RANGAP domain-like 1 isoform 3 VRHFsQSEDTGNE O55007 234 1 1.78E+05 102 -1.4 GIT2 614 G protein-coupled