Leader in Biomolecular Solutions for Life Science SULT2B1 Rabbit pAb Catalog No.: A7736 Basic Information Background Catalog No. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, A7736 neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure Observed MW (number and length of exons) is similar among family members. This gene sulfates 41kDa dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode Calculated MW different isoforms have been described. 39kDa/41kDa Category Primary antibody Applications WB, IHC, IF Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 6820 O00204 IHC 1:50 - 1:200 Immunogen 1:50 - 1:100 IF Recombinant fusion protein containing a sequence corresponding to amino acids 1-365 of human SULT2B1 (NP_814444.1). Synonyms SULT2B1;HSST2 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using SULT2B1 antibody (A7736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- Immunohistochemistry of paraffin- embedded human liver cancer using embedded human kidney cancer using embedded mouse lung using SULT2B1 SULT2B1 antibody (A7736) at dilution of SULT2B1 antibody (A7736) at dilution of antibody (A7736) at dilution of 1:100 (40x 1:100 (40x lens). 1:100 (40x lens). lens). Immunohistochemistry of paraffin- Immunofluorescence analysis of A549 cells embedded mouse kidney using SULT2B1 using SULT2B1 antibody (A7736). antibody (A7736) at dilution of 1:100 (40x lens). Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..