1 National Institutes of Science and Tecnology Edital N. 16/2014 MCTI/CNPq/ /CAPES/FAPs National Institute of Science and Tecnology in Stem Cells and Cell Therapy in Cancer INCTC Coordinator: Dimas Tadeu Covas Vice-Coordinator: Eduardo Magalhães Rego Headquarters: Hemocentro Foundation and Ribeirão Preto Medical School 2 SUMMARY A) Headquarters 4 B) Institutions, Laboratories and Associated Research Groups 4 C) Management Committee 4 D) Principal Investigators 5 D.1) Detailed description oF the proposing group 5 D.2) International Collaborators 14 D.3) Associated Researchers 14 D.4) Technical Team and post-graduate students associated with the project 15 D.5) ) Technical Team and post-graduate students associated with PIs 16 E) Organization and Functional Chart oF the Institute 17 F) Mechanisms that will be used to promote the interaction among groups and Forms 18 of interaction with groups abroad G) SpeciFication of the activities to be developed by team members 20 H) DeFinition of specific tasks of every participant entity 22 I) Description of the Program of the Institute 22 J) Detailing the Research Program 30 J.1) Description of the scientiFic project 31 J.2) Subprojects, objectives and goals 49 J.3) Methodology 62 J.4) Follow-up Indicators of the project 66 K) Detailing the program oF qualiFied proFessional training 67 L) Detailing the actions oF knowledge transFer to society 69 M) Detailing the actions oF knowledge transFer to business sector or Formulation oF 72 public policies N) Potential For generating patents 75 O) ScientiFic contributions and comparative analysis between the current and expected 77 situation P) Justified Budget 78 Q) Institutional counterpart 83 R) Description of the infrastructure 84 S) Implementation schedule 99 Appendix I – Formal agreement of institutions 100 Appendix II – Formal agreement of international collaborators 115 Appendix III – Team 126 Appendix IV – QualiFied professional training (completed) 128 Appendix V – QualiFied professional training (ongoing) 136 Appendix VI – Projects approved in the last 5 years 143 ReFerence 149 3 A) Headquarters • Hemocentro Foundation of Ribeirão Preto • Ribeirão Preto Medical School – HCFMRP- USP B) Associated Institutions, Laboratories or Research Groups • Ribeirão Preto Medical School – HCFMRP- USP • School oF Animal Husbandry and Food Engeneering oF Pirassununga – USP • School oF Veterinary Medicine – USP • Regional Blood Center of Ribeirão Preto – HCFMRP-USP • Primatology Center oF the Evandro Chagas Institute oF Belém (PA) • Institute oF Bioscience – USP • São Carlos Institute oF Physics – USP • Institute oF Biomedical Sciences – USP C) Management Committee The management committee will be integrated by the project coordinator (Prof. Dr. Dimas Tadeu Covas) and one representative From each oF the Five groups associated in this project: • School oF Veterinary Medicine oF São Paulo – ProF. Dr. Maria Angélica Miglino • School oF Animal Husbandry oF Pirassununga – ProF. Dr. Flávio Meirelles • Institute oF Biomedical Sciences – ProF. Dr. José Alexandre Marzagão Barbuto • Ribeirão Preto Medical School – ProF. Dr. Eduardo Magalhães Rego • São Carlos Institute oF Physics – ProF. Dr. Valtencir Zucolotto 4 D) Principal Investigators • Belinda Pinto Simões – Ribeirão Preto Medical School/USP • Dimas Tadeu Covas – Hemocentro Foundation of Ribeirão Preto • Eduardo Antonio Donadi – Ribeirão Preto Medical School/USP • Eduardo Magalhães Rego – Ribeirão Preto Medical School/USP • Flavio Vieira Meirelles – School of Animal Husbandry and Food Engeneering/USP • José Alexandre Marzagão Barbuto – Institute of Biomedical Sciences /USP • Klena Sarges Marruaz da Silva – Center of Primates of the Institute Evandro Chagas – Belém/PA • Lewis Joel Greene – Ribeirão Preto Medical School/USP • Lygia da Veiga Pereira – Institute of Bioscience/USP • Marco Antonio Zago – Ribeirão Preto Medical School/USP • Maria Angélica Miglino – School of Veterinary Medicine and Animal Husbandry/USP • Roberto Passetto Falcão – Ribeirão Preto Medical School/USP • Rodrigo Tocantins Calado – Ribeirão Preto Medical School/USP • Valtencir Zucolotto – São Carlos Institute of Physics/USP • Wilson Araújo da Silva Jr. – Ribeirão Preto Medical School/USP D.1) Detailed Description of the Proposing Group The Project will be coordinated by Prof. Dr. Dimas Tadeu Covas – CNPq researcher level 1B, graduated in Medicine at University oF São Paulo (USP) in (1981). Master’s (1986) and doctorate (1993) degrees in Medicine by USP, and he obtained livre-docência at the same University in 1999. Currently, Dr. Covas is a Full ProFessor at the Ribeirão Preto Medical School/USP, President-Director oF the Hemocentro Foundation oF Ribeirão Preto, Coordinator oF the Center For Cell-based Therapy (CTC-CEPID-FAPESP), and president oF the Brazilian Association oF Hematology, Hemotherapy and Cell Therapy (ABHH). He is a hematologist and hemotherapist, and develops research on the Following subjects: molecular and cellular biology, stem cells, erythrocytic and platelet antigens, viruses (HIV and HTLV), protein heterologous expression in cellular systems in vitro. He is Associate Editor oF Revista Brasileira de Hematologia e Hemoterapia, and associate editor oF the journal PLosOne; he was one oF the Founders oF the Escola Brasileira de Hematologia. In addition, Dr. 5 Covas is member oF Academia de Ciências de Ribeirão Preto. He is the coordinator oF the Postgraduate Program on Clinical Oncology, Stem Cells and Cell Therapy oF the FMRP/USP. He has also promoted the dissemination oF science and education, and organized postgraduate programs For teachers and elementary and high school students. He has edited two books, and one oF these was awarded Prêmio Jabuti in 2007. Dr. Covas has published 158 articles in specialized periodicals, 790 works in congresses including complete studies and abstracts, 16 book chapters, and 10 books. He has participated in 231 meetings in Brazil and abroad. His activities as advisor resulted in 22 Master's dissertations, 12 doctorate and 12 post-doctoral theses. In addition, Dr. Covas has advised 7 scientiFic initiation works, and has received 23 awards and/or honors. Lattes CV: http://lattes.cnpq.br/5244926449437566 The Following researchers also participate in this proposal as principal investigators: Prof. Dr. Belinda Pinto Simões – She received her doctorate in Medicine (Hematology) by the University oF São Paulo (1998) and her post-doctoral by the Ludwig-Maximilians- Universität München (2003). Currently she is a proFessor at the Ribeirão Preto Medical School oF the University oF São Paulo (FMRP-USP). She is the coordinator and supervisor oF the Unit oF Bone Marrow Transplantation oF the Hospital oF the FMRP-USP and was elected president oF the Brazilian Society oF Bone Marrow Transplantation. She has experience in Medicine, with emphasis on hematology, acute leukemias, bone marrow transplantation, and sickle cell disease. She has published 161 complete studies in journals and congresses and one book. She has participated in 22 scientiFic meetings and supervised 6 master’s dissertations, 1 doctorate thesis and she has received 5 awards. Lattes CV: http://lattes.cnpq.br/3238550237127398 Prof. Dr. Eduardo Antônio Donadi – Researcher level 1A oF CNPq. Dr. Donadi was graduated in Medicine by the Ribeirão Preto Medical School (1976), he has received his master’s degree by the Ribeirão Preto Medical School (1982) and his doctorate in Medical Practice by the same institution (1986). He conducted his post-doctoral activities in the Virginia Mason Research Center, in Seattle, in the years oF 1990 and 1991. He concluded his livre-docência in 1997 and currently he is a proFessor at the Division oF Clinical Immunology oF the Department oF Medical Practice oF the Ribeirão Preto Medical School- USP. He has experience in Medicine, in the Field oF Clinical Immunology, by researching, teaching and 6 treating patients with diseases related to immune system disorders, such as autoimmune diseases. His current research lines are related to the study oF the etiopathogenic aspects oF autoimmune diseases, transplantations, neoplastic and inFeccious diseases, Focusing on the evaluations oF diFFerential and particular gene expression oF candidate genes, polymorphisms oF genes involved in innate and acquired immune response, and also association oF genetic polymorphisms with gene expressions. Dr. Donadi has a particular interest in the study oF mechanisms that control the expression oF genes oF the major histocompatibility complex. He supervises postgraduate students in the Programs oF Basic and Applied Immunology and Medical Practice, both in the Ribeirão Preto Medical School oF University oF São Paulo. He has published 268 complete articles in journals, 1 book, 12 book chapters. He has participated in 63 national and international events. He has supervised 22 master’s dissertations, 30 doctorate and 30 post-doctoral theses. Lattes CV: http://lattes.cnpq.br/8778713382135771 Prof. Dr. Eduardo Magalhães Rego – CNPq researcher level 1C. He graduated in Medicine at Medicine Faculty oF Ribeirão Preto, University oF São Paulo, in 1988. He obtained his doctoral degree in Medical Clinic From the same institution (1997), and he did a post-doc at Memorial Sloan Kettering Cancer Center in New York, USA. Currently, Dr. Rego is Associate ProFessor at University oF São Paulo, and he works in the Field oF Hematology. His research line Focuses on acute leukemias, with special emphasis on Acute Promyelocytic Leukemia. He has published