Supplementary Materials: Distribution in Different Organisms of Amino Acid Oxidases with FAD or a Quinone As Cofactor and Their Role as Antimicrobial Proteins in Marine Bacteria Jonatan C. Campillo‐Brocal, Patricia Lucas‐Elío and Antonio Sanchez‐Amat * Table S1. Amino acid oxidases (AAOs) from microbial sources. Marine microorganisms are shown in bold. * cofactor and/or activity attributed for high similarity with LodA. ND, not determined. NA, not accessible. LAAOs, L‐amino acid oxidases. DAAOs, D‐amino acid oxidases. LASPOs, L‐aspartate oxidases. CTQ, cysteine tryptophilquinone. Microorganism Oligomeric Structure/Mass Accession Activity (Main Substrate) Cofactor Various Reference (Enzyme Name) Molecular Number AAOs with Quinone Cofactor (LodA‐Like Proteins) Marinomonas Homotetramer (80.9 × 4 kDa). Antimicrobial. Biofilms mediterranea MMB‐1 L‐Lysine ε‐oxidase (L‐Lys) CTQ Crystal structure solved, PDB AAY33849 [1,2] dispersion. Extracellular (LodA) ID: 2YMW Marinomonas Other substrates: Gly ethyl mediterranea MMB‐1 Glycine oxidase (Gly) CTQ 76.2 kDa ADZ90918 [3,4] ester (GoxA) Pseudoalteromonas Antimicrobial. Biofilms L‐Lysine ε‐oxidase (L‐Lys) * CTQ 110 kDa Q7X0I8 [5,6] tunicata D2 (AlpP) dispersion Chromobacterium Antimicrobial. Biofilms ND ND ND AAQ60932 [6] violaceum dispersion Antimicrobial. Biofilms Caulobacter crescentus ND ND ND NP_419374 [6] dispersion Pseudoalteromonas flavipulchra JG1 * L‐Lysine ε‐oxidase (* L‐Lys) * CTQ 77 kDa Antimicrobial. pI = 4.6 AFB71049 [7] (PfaP) Broad spectrum oxidase Antimicrobial. pI = 9.4. It Pseudoalteromonas (L‐Lys > L‐Met > L‐Glu > L‐Leu > L‐ ND 60 kDa contains a 9‐residues peptide NA [8] flavipulchra C2 Gln > L‐Tyr > L‐Phe) similar to AlpP S1 Table S1. Cont. Broad spectrum oxidase Pseudoalteromonas (L‐Met > L‐Gln > L‐Leu > L‐Phe > L‐ ND Oligomer (110 kDa) Antimicrobial NA [9] luteoviolacea Glu > L‐Trp) Antimicrobial. pI = 3.6. It Rheinheimera aquatica contains a 19‐resiudes * L‐Lysine ε‐oxidase (L‐Lys) * CTQ Monomer (71 kDa) NA [10] GR5 peptide similar to AlpP and LodA DAAOs Rubrobacter D‐Amino acid oxidase (neutral Thermostable. pH FAD Monomer (24,1 kDa) BAP18969 [11] xylanophilus (RxDAO) and basic D‐aa) opt = 7.5–10. Tª opt = 65 °C Arthrobacter D‐Amino acid oxidase (neutral protophormiae FAD Homodimer (34.6 × 2 kDa) pI = 4.2. pH opt = 6.5–8.5 AAP70489 [12] and hydrophobic D‐aa) (ApDAO) Homodimer (40 × 2 kDa). Rhodosporidium D‐Amino acid oxidase FAD Crystal structure solved, PDB P80324 [13] toruloides (RgDAO) ID: 1C0L Fusarium solani D‐Amino acid oxidase FAD 40 kDa Oxidizes Cephalosporin C P24552 [14] LASPOs NAD+ biosynthesis. Inhibited Monomer‐Dimer equilibrium by iminoaspartate, succinate, Bacillus subtilis 168 L‐Aspartate oxidase (L‐Asp) FAD NP_390665 [15] (55–110 kDa) fumarate, oxalacetate and D‐Asp Monomer‐Dimer equilibrium NAD+ biosynthesis. Inhibited (60.3–120 kDa). Crystal Escherichia coli K12 L‐Aspartate oxidase (L‐Asp) FAD by iminoaspartate, succinate P10902 [16,17] structure solved, PDB ID: and fumarate. pI = 5.6 1KNP Pyrococcus horikoshii Thermostable. pH L‐Aspartate oxidase (L‐Asp) FAD Homotrimer (51,925 × 3 kDa) O57765 [18] OT‐3 opt = 8.6. Tª opt = 90 °C Monomer (53.6 kDa). Crystal Other substrates: L‐Asn. Tª WP_0109792 Sulfolobus tokodaii L‐Aspartate oxidase (L‐Asp) FAD structure solved, PDB ID [19,20] opt = 79–87,5 °C 15 2E5V) S2 Table S1. Cont. Broad spectrum oxidase (L‐Leu > Pseudoalteromonas L‐Lys > L‐Tys > L‐Asn > L‐Gln > FAD 60 kDa Homology with LASPOs AJZ73816 [21] sp. B3 L‐Met > L‐cystine > L‐Arg > L‐Trp > L‐Glu) Fungal AAOs Broad spectrum oxidase (L‐His > aminobutyric > L‐canavanine > Neurospora crassa FAD ND L‐aa catabolism CAD21325 [22,23] L‐Tyr > D, L‐Ornithine > D, L‐Phe > L‐Leu) Broad spectrum oxidase (L‐His > aminobutyric > L‐canavanine > Aspergillus nidulans FAD ND L‐aa catabolism AAT84085 [24] L‐Tyr > D, L‐ornithine > D, L‐Phe > L‐Leu) Broad spectrum oxidase (L‐Phe, L‐ L‐aa catabolism. Nitrogen Laccaria bicolor S238N FAD ND DAA34975 [25,26] His, L‐Met, L‐Leu, and L‐Lys) mineralization. pI = 6.2 Broad spectrum oxidase (L‐Glu > L‐aa catabolism. Nitrogen Hebeloma L‐Gln > L‐Ornitina > L‐Asn > L‐ FAD 70 and 140 kDa mineralization. pI = 6.2. pH ADM80414 [25,26] cylindrosporum Leu > L‐His > L‐Phe) opt = 7–8 Broad spectrum oxidase (L‐Tyr > Aspergillus fumigatus L‐Phe > L‐Pro > L‐Ser > L‐Leu/L‐ ND ND Non active on D‐aa ND [27] P13 Ala > L‐Asp) Antitumoral. Other Homodimer (55 × 2 kDa). Trichoderma viride substrates: L‐Ornithine > L‐ L‐Lysine α‐oxidase (L‐Lys) FAD Crystal structure solved, PDB BAR88116 [28,29] (LysOX) Arg > L‐Phe. pI = 4.2. pH ID: 3X0V opt = 4.5‐10. Tª opt = 50 °C Trichoderma harzianum Broad spectrum oxidase (L‐Phe > Monomer‐Dimer equilibrium Biocontrol agent. FAD ADD91592 [30,31] ETS 323 L‐Lys > L‐Glu > L‐Ala) (63.5 kDa) Extracellular. pH opt = 7 S3 Table S1. Cont. L‐Lys biosensor. Other Saccharomyces cerevisiae L‐Lysine oxidase (L‐Lys) ND ND substrates: L‐Arg, L‐Asn. pH NA [32] opt = 7,5. Tª opt = 30 °C Other substrates: L ‐Phe and L‐Tryptophan oxidase (L‐Trp and Coprinus sp. SF‐1 (Tod) FAD 68 KDa L‐Tyr. Tª opt = 35–43 °C. pH NA [33] derivatives) opt = 7 Other enzymes with AAO activity Homotetramer (42 × 4 kDa). Bacillus subtilis 168 Other substrates: Sarcosine, Glycine oxidase (Gly) FAD Crystal structure solved, PDB O31616 [34,35] (GoxB) N‐ethyl‐Gly, D‐Pro, D‐Ala ID: 1NG4 Geobacillus Other substrates: Sarcosine, kaustophilus HTA426 Glycine oxidase (Gly) FAD Homotetramer (42 × 4 kDa) N‐ethyl‐Gly, D‐Pro, D‐Ala. BAD74908 [36] (GoxK) Thermostable Other substrates: L‐Pro, Bacillus sp. B‐0618 Monomer (43.8 kDa). Crystal N‐ethyl‐Gly, N‐methyl‐ D L‐ Sarcosine oxidase (sarcosine) FAD BAA03967 [37,38] (MSox) structure solved, PDB ID: 2A89 Ala, N‐methyl‐D L‐Val, N‐methyl‐L‐Leu Antioxidant and microbial Streptococcus Aminoacetone oxidase 43 kDa. Crystal structure competition. Other substrates: oligofermentans FAD ACA52024 [39–41] (aminoacteone) solved, PDB ID: 4CNK L‐Asp > L‐Trp > L‐Lys > L‐ (SoAAO) Ile > L‐Arg > L‐Apn > L‐Gln Broad spectrum oxidase (all the Homodimer (53 × 2 kDa). Rhodococcus opacus pI = 4,8. pH opt = 8–9. proteinogenic aa except Gly, FAD Crystal structure solved, PDB AAL14831 [42,43] DSM 43250 Tª opt = 30 °C L‐Thr and L‐Pro) ID: 2JB2 pI = 4.8. pH opt = 8–8,5. Broad spectrum oxidase (L‐Ala > Rhodococcus sp. AIU N‐terminal homology with Nα‐Z‐L‐Lys > L‐His > L‐Tyr > L‐ FAD Homodimer (51 × 2 kDa) NA [44,45] Z‐35‐1 Rhodococcus opacus DSM ornithine > L‐Gln) 43250 LAO. pH opt = 6–8.5. Tª opt = 45 °C. Broad spectrum oxidase (L‐Ala > Rhodococcus sp. AIU N‐terminal homology with L‐Gln > Nα‐Z‐L‐Lys> L‐rn > L‐ FAD Homodimer (52,5×2 kDa) NA [45] LAB‐3 Rhodococcus opacus DSM Arg > L‐Phe > L‐Met > L‐Lys) 43250 LAO. S4 Table S1. Cont. Broad spectrum oxidase (L‐Leu > Other substrates: D‐isomers. Bacillus carotarum 2Pfa FAD Homodimer (54×2 kDa) NA [46] L‐Lys > L‐Arg > L‐Met > L‐Asn) pI = 4.8. pH opt = 8–8.5 Broad spectrum oxidase (all the Cellulomonas cellulans proteinogenic aa except Gly, L‐Pro FAD 55 kDa Extracellular. pH opt=6,5‐7,5 NA [47] AM8 and L‐Thr) Broad spectrum oxidase (all the Corynebacterium sp. proteinogenic aa except L‐Asp, ND 130‐140 kDa L‐aa catabolism NA [48] A20 L‐Thr, L‐Pro and Gly) Broad spectrum oxidase (L‐Leu > Morganella morganii FAD ND Tª opt = 35–43 °C NA [49] L‐Phe > L‐Trp > L‐Met > L‐Tyr) Hexamer α2β2γ2 [(42 + 17 + 10) L‐Glu biosensor. Streptomyces sp. X‐119‐6 L‐Glutamate oxidase (L‐Glu) FAD × 2 kDa]. Crystal structure Extracellular. pH opt = 7. Tª BAB93449 [50–52] solved, PDB ID: 2E1M opt = 58 °C pI = 6.2. pH opt = 6.5–8. Tª Streptomyces endus L‐Glutamate oxidase (L‐Glu) FAD Dimer (45 × 2 KDa) NA [53] opt = 30–45 °C Streptomyces platensis Heterotrimer of 78 kDa (39, 19 L‐Glutamate oxidase (L‐Glu) FAD L‐Glu biosensor AAK15071 [54] NTU3304 and 16 kDa) Streptomyces violascens L‐Glutamate oxidase (L‐Glu) FAD Monomer (60 kDa) Other substrates: L‐Gln NA [55] Tetramer α2β2 Streptomyces sp. Z‐11‐6 L‐Glutamate oxidase (L‐Glu) FAD Extracellular NA [56] [(25 + 22.5) × 2 kDa] Homodimer (54.5 × 2 kDa). Other substrates: Pseudomonas sp. AIU L‐Lysine FAD Crystal structure solved, PDB L‐Ornithine > L‐Arg. Induced BAO51829 [57,58] 813 α‐oxidase/monooxygenase (L‐Lys) ID: 3WE0 by L‐Lys. pI = 4.6. pH opt = 7 Other substrates: L‐Tyr > L‐ L‐Phenylalanine oxidase Heterodimer α2β2 [(10 + 60) × Pseudomonas sp. P‐501 Met > L‐NorLeu > L‐Trp. L‐Phe (deaminating and FAD 2 kDa]. Crystal structure BAD66877 [59–61] (PAO) is mainly oxygenated and L‐ decarboxylating) (L‐Phe) solved, PDB ID: 3AYJ Met is mainly oxidized Pseudomonas savastanoi Tryptophan 2‐monooxygenase Biosynthesis of indoleacetic FAD 62 kDa EFW87519 [62] (TMO) (L‐Trp) acid S5 Table S1. Cont. Other substrates: L‐Tyr, L‐ Trp, L‐Met and L‐Phe. L‐Phe and L‐Tyr are mainly Ralstonia solanacearum L‐Tryptophan monooxygenase Heterodimer α2β2 FAD oxygenated. L‐Met is mainly NA [63] (PTMO) (L‐Trp) [(9.2 + 64.5) × 2 kDa]. oxidized. L‐Trp is both oxygenated and oxidized. Homology with PAO Chromobacterium L‐Tryptophan oxidase Violacein biosynthesis. pH FAD 48 KDa Q9S3V1 [64] violacium (VioA) (L‐Trp and derivatives) opt = 9,25 Lechevalieria L‐Tryptophan oxidase aerocolonigenes ATCC FAD Homodimer (54 × 2 kDa) Rebeccamycin biosynthesis BAC15750 [65] (L‐Trp and derivatives) 39243 (RebO) Staurosporine biosynthesis.