CCNE1 monoclonal antibody, clone Gene Symbol: CCNE1 DDF-3 Gene Alias: CCNE Catalog Number: MAB19936 Gene Summary: The protein encoded by this gene belongs to the highly conserved cyclin family, whose Regulatory Status: For research use only (RUO) members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins Product Description: Rabbit monoclonal antibody function as regulators of CDK kinases. Different cyclins raised against synthetic peptide of human CCNE1. exhibit distinct expression and degradation patterns Clone Name: DDF-3 which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and Immunogen: A synthetic peptide corresponding to functions as a regulatory subunit of CDK2, whose human CCNE1. activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is Host: Rabbit degraded as cells progress through S phase. Overexpression of this gene has been observed in many Reactivity: Human tumors, which results in chromosome instability, and thus may contribute to tumorigenesis. This protein was Applications: Flow Cyt, ICC, IF, IP, WB-Ce found to associate with, and be involved in, the (See our web site product page for detailed applications phosphorylation of NPAT protein (nuclear protein information) mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a Protocols: See our web site at critical role in promoting cell-cycle progression in the http://www.abnova.com/support/protocols.asp or product absence of pRB. Two alternatively spliced transcript page for detailed protocols variants of this gene, which encode distinct isoforms, Form: Liquid have been described. Two additional splice variants were reported but detailed nucleotide sequence Purification: Affinity purification information is not yet available. [provided by RefSeq] Isotype: IgG Recommend Usage: Immunocytochemistry (1:50-1:200) Immunofluorescence (1:50-1:200) Immunoprecipitation (1:50) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Storage Instruction: Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing. Entrez GeneID: 898 Page 1/1 Powered by TCPDF (www.tcpdf.org).