Proceedings of the 4th WORKSHOP ON AGRI-FOOD RESEARCH 4th Workshop on Agri-food Research-WiA.15. Cartagena, Murcia, Spain. 11-12 May 2015 Proceedings of the 4th WORKSHOP ON AGRI-FOOD RESEARCH Editores y Comité Científico Organizador Dr. Francisco Artés-Hernández Dr. Marcos Egea Gutiérrez-Cortines Dr. Juan Antonio Fernández-Hernández Dr. Alain Baille Dr. Javier Calatrava Programa de doctorado en Técnicas Avanzadas en Investigación y Desarrollo Agrario y Alimentario (TAIDA). Escuela Técnica Superior de Ingeniería Agronómica (ETSIA). Universidad Politécnica de Cartagena (UPCT) . Doctorate Program in Advanced Techniques for Research and Development in Food and Agriculture (TAIDA). Escuela Técnica Superior de Ingeniería Agronómica (ETSIA). Universidad Politécnica de Cartagena (UPCT) . http://taidaa.upct.es/ 4th Workshop on Agri-food Research - WiA.15. Cartagena, Murcia, Spain. 11-12 May 2015 Acknowledgements The Organizing Committee acknowledges the Escuela Técnica Superior de Ingeniería Agronómica -ETSIA- and the Institute of Plant Biotechnology -IBV- of the Universidad Politécnica de Cartagena – UPCT- their assistance in making possible this event. To Mare Nostrum Campus -CMN- the dissemination done. Funding received from the International Doctorate School of UPCT is also appreciated. © 2016, Francisco Artés-Hernández, Marcos Egea Gutiérrez-Cortines, Juan Antonio Fernández- Hernández, Alain Baille , Javier Calatrava, (editores y comité organizador) © 2016, Programa de doctorado en Técnicas Avanzadas en Investigación y Desarrollo Agrario y Alimentario. TAIDA, UPCT. © 2016, Universidad Politécnica de Cartagena CRAI Biblioteca Plaza del Hospital, 1 30202 Cartagena 968325908 [email protected] Primera Edición, 2016 ISBN: 978-84-608-5399-2 Esta obra está bajo una licencia de Reconocimiento-NO comercial-Sin Obra Derivada (by-nc-nd): no se permite el uso comercial de la obra original ni la generación de obras derivadas. http://creativecommons.org/licenses/by-nc-nd/4.0 2 | 269 4th Workshop on Agri-food Research - WiA.15. Cartagena, Murcia, Spain. 11-12 May 2015 Preface As Convener of the Organizing and Scientific Committee of the 4th Workshop on Agri- Food Research (WIA.15) and Coordinator of the Doctoral Program in Advanced Techniques for Research and Development in Food and Agriculture (TAIDA), I am very pleased to see embodied in this book the excellent presentations conducted by PhD candidates of our Program. Presentations showed a great scientific interest and reflect the high potential of the Research Groups that belong to the several departments and institutions integrated into our PhD Program (UPCT, CEBAS-CSIC, and IMIDA). We expect that during the celebration of this Workshop, the coexistence and exchange of ideas and experiences among PhD candidates, research groups, researchers, technicians, etc.. has been favored. The scientific program is structured into four sessions: Agrifood Biotechnology, Food Technology and Engineering, Plant Production Technology and Engineering and Agroforestry and Economy Engineering, depending on the topics of interest involved in our Program. We also had three general invited lectures to strengthen the knowledge that researchers are gaining in their formation during this stage and we consider very useful for other researchers. The Scientific Organizing Committee consider that it is necessary that PhD candidates undertake this type of formative training activities to acquire the typical skills of a PhD remarking how to summarize their results, highlight the importance of them, presenting and disseminating clearly and concisely to a diverse audience in a limited time, as usually happens in the presentations at worldwide scientific conferences. Conclusively, I want to thank, on behalf of the Scientific Committee, the participation of PhD candidates and their advisors, as well as the high quality of the presentations. Francisco Artés-Hernández PhD Convener of the 4th Workshop on Agri-Food Research (WIA.15) 3 | 269 Index Keynote speakers: • Research and development needs to reduce global food losses Dr. Jorge M. Fonseca 11 Agro-industry Official. Food and Agriculture Organization ‐ FAO (Rome, Italiy). • The world 2.0 as an emergent tool for science dissemination Dr. José Manuel López Nicolás 12 Biochemistry and Molecular Biology department. University of Murcia (Spain). In charge of the several times awarded “Scientia" blog (‘Bitácoras’, ‘20 blog’, ‘ASEBIO’, etc). • From hypothesis construction through experimental execution to result publication and citation 14 Dr. Mark D. Wilkinson BBVA Chair of Biological Informatics. Distinguished researcher Isaac Peral at Centro de Biotecnología y Genómica de Plantas (CBGP). Universidad Politécnica de Madrid –INIA. Invited expert at W3C’s Semantic Web in Health Care and Life Sciences Interest Group and member of the Editorial Board of the Journal of Biomedical Semantics. 4 | 269 4th Workshop on Agri-food Research - WiA.15. Cartagena, Murcia, Spain. 11-12 May 2015 INDEX ORAL SESSION I: Food Technology and Engineering 15 Continuous microwave heating effects on the quality of carrot and tomato smoothie 16 M. Arjmandi, E. Aguayo, M. Otón, F. Artés Effect of isothermal and non-isothermal treatments on the viability and stress response of foodborne pathogen and spoilage microorganisms 20 J.P. Huertas, A. Esnoz, A. Palop Heat resistance of Listeria monocytogenes in sterile distilled water in the presence of nanoemulsion of D-limonene 24 J. Maté, P.M. Periago, A. Palop Innovative technology for industrial manufacturing of gluten-free pasta sheets 27 S. Soto, M. Boluda, A. López Effect of application of elicitors on the yield and quality of tomato 31 V. Hernández, P. Hellín, J. Fenoll, M.P. Flores Water stress to enhance vitamin C in extra early nectarines 35 N. Falagán, F. Artés, P.A. Gómez, F. Artés-Hernández, J.M. de la Rosa, E. Aguayo Phytoprostanes determination in almonds depending on the cultivar and growing conditions (conventional versus ecological) 39 A.M. Carrasco del Amor, A. Gil Izquierdo, E. Aguayo Epiphytic microbial load changes of natural purple fresh vegetables smoothies during shelf life 43 G. González-Tejedor, F. Artés-Hernández, P.S. Fernández Effect of high pressure homogenization against pasteurization on a nutraceutical product with a high 10-hydroxy-2-trans-decenoic acid content and vitamin C 47 A. García-González, P.A. Gómez, E. Aguayo Optimization of chlorine sanitation to preserve quality of minimally processed Vicia fabe seeds 51 M. Otón, F. Artés, F. Artés-Hernández Discrimination of volatile compounds at harvest in melon identified by SPME and GC-MS 55 L.A.Chaparro- Torres, J.P Fernández-Trujillo Sodium acidified chlorite and ozonated water as alternative sanitizing techniques for minimally processed broccoli 59 J. Navarro-Rico, G.B. Martínez-Hernández, P. Gómez, F. Artés, F. Artés-Hernández ORAL SESSION II: Agrifood Biotechnology 63 "Itumthirteen" & "Itumfourteen": a bet on the Muscat grape flavor 64 M. Tornel, J. Carreño Identification of DNA from Antirrhinum linkianum introgressed in A. majus with common molecular markers 68 V. Ruiz-Hernández, J. Weiss, M. Egea-Cortines MISTRAL 30®, new platerine obtained from IMIDA-NOVAMED breeding program 71 A. Carrillo-Navarro, A. Guevara-Gázquez , J. Cos-Terrer Expression patterns of antioxidant systems in pioneer plants present in mine tailings contaminated with high concentrations of heavy metals 74 A. López-Orenes, H. Oliveira, C. Santos, M.A. Ferrer, A.A. Calderón Genetic analysis of traits of interest in wine grapes 78 A. Bayo-Canha, J.I. Fernández-Fernández, A. Martínez-Cutillas, L. Ruiz-García Estimates of heritabilities and genetic correlations of carcass quality traits in a reared gilthead seabream (Sparus aurata L.) population sourced from three broodstocks 82 M. García-Celdrán, G. Ramis, E. Armero 5 | 269 4th Workshop on Agri-food Research - WiA.15. Cartagena, Murcia, Spain. 11-12 May 2015 Sweet cherry behaviour in the climatic conditions of the Region of Murcia 86 G. López-Ortega, F. García, C. Frutos, A. Bayo-Canha, D. Frutos Using 23S rDNA to identify contaminations of Escherichia coli in Agrobacterium tumefaciens cultures 90 M. Manchado-Rojo, J. Weiss , M. Egea-Cortines Differential growth of the main parts of the carcass rabbit 93 A. Martínez-Bas , E. Armero, M. Kessler Trailing plants, identification of T-DNA mutants and introgressions of Antirrhinum linkianum in A.majus 97 R. Alcantud, J.Weiss, M. Egea-Cortines Effect of concentration of IBA during rooting phase in vitro of Limonium sinuatum on the survival and quality of the plant 99 J. Sánchez, A.A. Calderón Quantitative evaluation of bias in PCR amplification and Next Generation Sequencing derived from metabarcoding samples 103 M. Pawluczyk, J.Weiss, M.G. Links, M.E. Aranguren, M.D. Wilkinson, M. Egea-Cortines ORAL SESSION III: Vegetal Production Technology and Engineering 107 Influence of Phragmites australis on the seasonal evolution of biogeochemical conditions (pH/Eh) and N cycling in eutrophic wetlands 108 M.C. Tercero-Gómez, J. Álvarez-Rogel, M.I. Arce, M.J. Delgado, H.M. Conesa, I. Párraga-Aguado, M.N. González-Alcaraz Evolution of Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) resistance to diamide insecticides over the last 5 years 112 L. García-Vidal, M.R. Martínez-Aguirre , P. Bielza Genetic variability of tolerance to spinosad and emamectin benzoate in different Mediterranean populations of Orius laevigatus (Fieber) (Hemiptera: Anthocoridae) 116 V. Balanza, J.E. Mendoza, P. Bielza Cold-tolerance variability