Catalog # Aliquot Size U219-911-05 3 x 5 nmol U219-911-20 3 x 20 nmol U219-911-50 3 x 50 nmol UBE2E3 (UBCH9) siRNA Set I siRNA duplexes targeted against three exon regions Catalog # U219-911 Lot # Z2109-21 Specificity Formulation UBE2E3 (UBCH9) siRNAs are designed to specifically The siRNAs are supplied as a lyophilized powder and knock-down human UBE2E3 (UBCH9) expression. shipped at room temperature. Product Description UBE2E3 (UBCH9) siRNA is a pool of three individual Reconstitution Protocol synthetic siRNA duplexes designed to knock-down Briefly centrifuge the tubes (maximum RCF 4,000g) to human UBE2E3 (UBCH9) mRNA expression. Each siRNA is collect lyophilized siRNA at the bottom of the tube. 19-25 bases in length. The gene accession number is Resuspend the siRNA in 50 µl of DEPC-treated water NM_006357. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot UBCH9; UbcM2 1x stock solutions for storage. Storage and Stability The lyophilized powder is stable for at least 4 weeks at Related Products room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or Product Name Catalog Number below -20oC. After resuspension, siRNA stock solutions ≥2 UBE2E3 (UBCH9) Protein U219-30H µM can undergo up to 50 freeze-thaw cycles without UBE2F Protein U220-30H significant degradation. For long-term storage, it is UBE2G1 (UBC7) Protein U221-30H recommended that the siRNA is stored at -70oC. For most UBE2H Protein U223-30H favorable performance, avoid repeated handling and UBE2I Protein U224-30H multiple freeze/thaw cycles. UBE2J1 Protein U225-30G UBE2K (UBC1) Protein U227-30H Scientific Background UBE2L6 (UBCH8) Protein U229-30H UBE2E3 also known as UBCH9 or ubiquitin-conjugating enzyme E2E 3 is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination, the covalent attachment of the protein ubiquitin (Ub) to other cellular proteins is an UBE2E3 (UBCH9) siRNA Set I important cellular mechanism for targeting abnormal or siRNA duplexes targeted against three exon regions short-lived proteins for degradation that involves at least three classes of enzymes: ubiquitin-activating enzymes, or Catalog # U219-911 E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin- Lot # Z2109-21 protein ligases, or E3s(1). UBE2E3 is highly expressed in Packaging Specifications 2.5 nmol/tube for 3 x 5nmol skeletal muscle (2). UBE2E3 is essential for the proliferation Format Lyophilized powder of RPE-1 cells and is downregulated during RPE layer Stability 1yr at -70oC from date of shipment maturation in the developing mouse eye. These findings Storage & Shipping The lyophilized powder is stable for at indicate that UBE2E3 is a major enzyme in modulating the least 4 weeks at room temperature. It is recommended that the lyophilized balance between RPE cell proliferation and and resuspended siRNAs are stored at differentiation. or below -20oC. After resuspension, siRNA stock solutions ≥2 µM can References undergo up to 50 freeze-thaw cycles 1. Matuschewski, K.et.al: Identification of a novel family without significant degradation. For of ubiquitin-conjugating enzymes with distinct amino- long-term storage, it is recommended that the siRNA is stored at -70oC. For terminal extensions. J. Biol. Chem. 271: 2789-2794, most favorable performance, avoid 1996. repeated handling and multiple 2. Ito, K.et.al: cDNA cloning, characterization, and freeze/thaw cycles. chromosome mapping of UBE2E3 (alias UbcH9), encoding an N-terminally extended human ubiquitin- conjugating enzyme. Cytogenet. Cell Genet. 84: 99- 104, 1999. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .