A COMPARATIVE STUDY ON WOUND HEALING IN LAMBS AND HOGGETS FOLLOWING MULESING L.C.WILSON, C. ESPINOZA1, P.A. WINDSOR2 and S. LOMAX3 Summary The aims of this experiment were to assess the rate of healing of mulesing wounds based on treatment with a topical anaesthetic (TA), and to compare the rate of wound healing between lambs and hoggets to develop an understanding of possible age effects. Lambs and hoggets were weighed and mulesed (Day 0), with digital photographs of wounds taken at days 0, 14 and 28. Wound surface area (WSA) was then measured using image analysis software. This is a comparative study, comparing data collected from hoggets in 2012 and previously published data on lambs from a trial conducted in 2006. The 2006 component of this trial (published by Lomax et al., 2008) found a significant effect of TA on reducing the rate of wound healing in lambs. The current study did not find a significant effect on wound healing of treatment with TA. However there was a significant difference between age groups, indicating that this is a key area for further investigation. I. INTRODUCTION The proposed research aimed to assess the effect of age on the rate of healing of the mulesing wound. It was hypothesised that in older sheep, wound healing time may be reduced due to an increased body size (relative to wound size) and the presence of more subcutaneous fat to protect the open wound (Dwyer, 2008). This hypothesised effect would also be compounded due to the ability of producers to more conservatively, or selectively mules older sheep as with age improved body condition, the breech wrinkle score can reduce (Brown et al., 1966). In this way, the wound size to body size ratio may be reduced even further, as lambs are able to be mulesed based on their specific conformation, rather than on a prediction. Both Grant (2004) and Lomax et al. (2008) propose that it is in the best interest of the animal if the mulesing cut is as small as possible, whilst still able to provide protection against flystrike. This may be easier to achieve on older lambs as breech wrinkles are minimised as body condition improves. Due to a larger body size to wound size ratio in older sheep, it was expected that with increasing age, wound healing time would be reduced. It has been shown previously by Lomax et al. (2008) that initial wound surface area (WSA) had a significant effect on the rate of wound healing, with smaller wounds taking significantly less time to heal than larger wounds. The effect of a topical anaesthetic (TA) TriSolfen® on the rate of healing of mulesing wounds was also investigated. TriSolfen® was licensed for use in sheep in 2006 as a post- operative topical anaesthetic in the form of a spray. Current industry regulations do not require the provision of pain relief post mulesing to sheep under six months of age (CSIRO, 2006), however the adoption of Tri-Solfen® as standard practice by an estimated 70% of Australian farmers shows that welfare is a primary concern - regardless of economic benefit (Windsor & Lomax, 2013). The research targeted Merino lambs as this breed are predisposed to flystrike due to their heavily wrinkled breech area. The research aimed to determine whether there is an advantage to postponing mulesing from either an economic, welfare or management perspective. As this was a comparative study, with the two trials conducted nearly six years apart, at different properties and using different contractors, there are a large number of variables between the two experiments. For any future investigation into the effect of age on wound healing post- mulesing, it would be ideal to conduct mulesing trials on lambs and hoggets simultaneously, under the same conditions and using the same mulesing contractor. II. MATERIALS AND METHODS Animals and housing A trial was conducted on 200 Merino ewe hoggets aged 10 months at The University of Sydney’s ‘Arthursleigh’ property near Marulan, New South Wales, Australia, in winter 2012. The hoggets had previously been hot-iron tail-docked before 2 months of age with wounds completely healed at the time of experiment. The hoggets were also crutched one week prior to mulesing. Mean weight of hoggets at Day 0 was 27.4 ± 3.5 kg. Experimental protocol was approved by the Animal Ethics Committee of The University of Sydney. The aims of this experiment were: (1) to assess rate of wound healing based on treatment with and without topical anaesthetic (TA) and (2) to compare the rate of wound healing between lambs and hoggets in order to develop an understanding of possible age effects. Data was collected from hoggets to be compared to previous records on lambs to determine whether there was a difference that should be further explored. The previous data on lambs was published in a paper by Lomax et al. (2008), which employed a similar experimental method, with fewer animals. Details of each trial are outlined in Table 1. Table 1. Age, sex, number, location and period of data collection of sheep (lambs; hoggets) involved in the assessment of mulesing wounds Cohort Age Sex Number of Location Period of data (months) animals collection 1 (lambs) 1.5 Mixed 100 ‘Yerilla’ (Central Spring 2006 Tablelands, New South Wales, Australia) 2 (hoggets) 10 Female 200 ‘Arthursleigh’ (Southern Winter 2012 Highlands, New South Wales, Australia) Experimental design and treatments The cohort of hoggets was divided into two treatment groups, to be compared with data collected from a previous trial on lambs. The treatment groups are outlined in Table 2. Those animals treated with a topical anaesthetic received the immediate application of the topical anaesthetic (TA) gel (Tri-Solfen®) to the entire area of exposed wound. Table 2. Treatment groups Cohort Treatment Number of animals 1 (lambs) Mulesing only (M) 50 1 (lambs) Mulesing with Topical anaesthetic (MTA) 49 2 (hoggets) Mulesing only (M) 100 2 (hoggets) Mulesing with Topical anaesthetic (MTA) 100 Wound healing On the first day of the trial, hoggets were mustered from their grazing paddocks into the shearing shed where they were moved into small holding pens. The hoggets were individually moved through an electronic sheep handler (Hdale Engineering Ltd, Model no: CWC RC) at which time they were weighed. Hoggets were then moved onto an electronic VE-conveyor, ear-tagged, and placed into dorsal recumbency with their hindlegs secured into leg hooks. Hoggets (total n = 200) were randomly assigned to one of two treatments as they moved through the conveyor, by drawing the relevant number out of a hat: (1) mulesing only (M) (n = 100); and (2) mulesing with the immediate application of a topical anaesthetic (TA) gel (Tri-Solfen®, Bayer Animal Health, Pymble, Australia) (MTA) (n = 100). Sham-mulesing involved manipulation of the breech skin, and making contact with the skin of the breech area using shears, without incising the skin. Mulesing was performed by an accredited and experienced property employee using the standard ‘V’ modified mulesing technique. Hoggets receiving the MTA treatment were immediately (within seconds) treated with approximately 12 ml of TA over the entire mulesing wound and wound edge, as per industry best practice. Tag number and weight were recorded for each individual animal to allow repeat recording of weight and wound healing over time. Following treatment, animals were released onto the grazing paddocks where they remained until subsequent data was collected. Wound healing Wound size was assessed by measuring the surface area of the mulesing wounds of M and MTA hoggets at Week 0, 2 and 4. Wound surface area (WSA) was measured by taking a digital photograph of the wound using a digital SLR camera. A linear scale (30 cm ruler) and animal identification number were included in the photograph. In each photograph, the scale was placed above the wound, against the wool. Image analysis software (Image J 1.47H, Wayne Rasband, National Institutes of Health, U.S.A.) was then used to trace the wound edge and therefore calculate the area of each wound in cm2 based on the included scale. Using this software, measurements were made for each wound at all time points - by two separate assessors blinded to treatment. Wound areas that varied by greater than 10% we re- measured by both assessors until the areas aligned <10%. The wound area results of both assessors, for each animal at each time point, were results averaged to obtain a final measurement for statistical analysis. Fig 1. Screenshot of WSA measurement from image analysis program, ImageJ Statistical Analysis Wound size data were analysed using an Analysis of Variance approach using statistical program GenStat® (13th Ed. VSN International). For the analysis of wound size, the effect of treatment was investigated. For comparison of lambs and hoggets, age and treatment and their interaction were investigated. Wound size data were analysed as a difference to initial wound size (Day O) to determine the rate of wound healing. Data were tested for normality using the Anderson-Darling Test (5%). All data analysed followed a normal distribution and therefore, did not need to be transformed. For all statistical calculations, P-values less than 0.05 were considered statistically significant. III. RESULTS In the comparison between the 2006 lamb trial and 2012 hogget trial, the effect of treatment, age and the interaction between treatment and age were included in the statistical model. The interaction between age and treatment did not have a significant effect (P > 0.05) and was therefore removed from the model. In hoggets, treatment did not have a significant effect on the rate of wound healing, or the percentage change over time. By day 28, wounds treated and untreated healed by 83.95% and 81.64% respectively.