Catalog # Aliquot Size P74-911-05 3 x 5 nmol P74-911-20 3 x 20 nmol P74-911-50 3 x 50 nmol PKC theta siRNA Set I siRNA duplexes targeted against three exon regions Catalog # P74-911 Lot # Z2073-80 Specificity Formulation PKC theta siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human PKC theta expression. shipped at room temperature. Product Description Reconstitution Protocol PKC theta siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human PKC collect lyophilized siRNA at the bottom of the tube. theta mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The gene accession number is NM_006257. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage. PRKCQ, PRKCT, MGC126514, MGC141919, nPKC-theta Related Products Storage and Stability The lyophilized powder is stable for at least 4 weeks at Product Name Catalog Number room temperature. It is recommended that the PKC alpha, Active P61-10G lyophilized and resuspended siRNAs are stored at or PKC alpha, Active P61-18G below -20oC. After resuspension, siRNA stock solutions ≥2 PKC beta I, Active P62-10G µM can undergo up to 50 freeze-thaw cycles without PKC beta II, Active P63-10G significant degradation. For long-term storage, it is PKC delta, Active P64-10G recommended that the siRNA is stored at -70oC. For most PKC epsilon, Active P65-10G favorable performance, avoid repeated handling and PKC eta, Active P67-10G multiple freeze/thaw cycles. PKC gamma, Active P66-10G PKC iota, Active P68-10G Scientific Background PKC mu, Active P72-1G Protein Kinase C, theta (PKC) is important component in PKC nu, Active P73-10G the intracellular signaling cascade (1). Recent studies PKC theta, Active P74-10G have suggested that local accumulation of fat PKC zeta, Active P75-10G metabolites inside skeletal muscle may activate a serine kinase cascade involving PKC-theta leading to defects in PKC theta siRNA Set I insulin signaling and glucose transport in skeletal muscle siRNA duplexes targeted against three exon regions (2). Insulin resistance plays a primary role in the Catalog # development of type 2 diabetes and may be related to P74-911 alterations in fat metabolism. PKC-theta is a crucial Lot # Z2073-80 component mediating fat-induced insulin resistance in Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol skeletal muscle and is a potential therapeutic target for Format Lyophilized powder o the treatment of type 2 diabetes (2). Stability 1yr at -70 C from date of shipment Storage & Shipping The lyophilized powder is stable for at least 4 weeks at room temperature. It is References recommended that the lyophilized and resuspended siRNAs are stored at 1. Manicassamy, S. and Sun, Z. The critical role of or below -20oC. After resuspension, protein kinase C-theta in Fas/Fas ligand-mediated siRNA stock solutions ≥2 µM can apoptosis. J. Immunol. 2007;178(1):312-9. undergo up to 50 freeze-thaw cycles 2. Kim, J K. et al: PKC-theta knockout mice are without significant degradation. For long-term storage, it is recommended protected from fat-induced insulin resistance. J. Clin. that the siRNA is stored at -70oC. For Invest. 2004;114(6):823-7. most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .