Methods in Molecular Biology 1349 Alejandro Brun Editor Vaccine Technologies for Veterinary Viral Diseases Methods and Protocols M ETHODS IN MOLECULAR BIOLOGY Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hat fi eld, Hertfordshire, AL10 9AB, UK For further volumes: http://www.springer.com/series/7651 Vaccine Technologies for Veterinary Viral Diseases Methods and Protocols Edited by Alejandro Brun CISA-INIA, Valdeolmos, Madrid, Spain Editor Alejandro Brun CISA-INIA Valdeolmos , Madrid , Spain ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3007-4 ISBN 978-1-4939-3008-1 (eBook) DOI 10.1007/978-1-4939-3008-1 Library of Congress Control Number: 2015949635 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifi cally the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfi lms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specifi c statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com) Pref ace A great amount of knowledge has been generated in recent years on veterinary vaccine technologies, so presently many different antigen delivery systems are available for vaccine research, in particular against viral diseases. This book was intended to review most popular antigen production and delivery strategies that have been tested in veterinary species. Viral vectors as well as genetic and protein subunit vaccines or large-scale protein production systems are considered among the different chapters of the book. Our aim has been to facilitate the access to real and well-established protocols to those beginning in this interest- ing and laborious fi eld as well as to provide basic knowledge when attempting a novel vac- cine design or platform. This book, in addition, will provide an updated view of most options available when vaccine development is considered. In spite of the number of alter- natives available for vaccine design, few of them often reach market and commercialization, but, nonetheless, the data obtained along experimental trials contribute to the exploration and understanding of the immune mechanisms and immune correlates relevant in protec- tion among different animal species. Therefore this book also could be of help to those interested in basic and applied immunology, since these technologies represent interesting tools to induce or modulate different host immune mechanisms. Valdeolmos, Madrid, Spain Alejandro Brun v Contents Preface. v Contributors . ix 1 Vaccines and Vaccination for Veterinary Viral Diseases: A General Overview . 1 Alejandro Brun 2 Using IC-Tagging Methodology for Production and Purification of Epitope-Loaded Protein Microspheres for Vaccination . 25 Natalia Barreiro- Piñeiro , Rebeca Menaya-Vargas , Alberto Brandariz-Núñez , Iria Otero- Romero , Irene Lostalé-Seijo , Javier Benavente , and José M. Martínez-Costas 3 Plant-Based Vaccine Antigen Production. 35 Hoang Trong Phan and Udo Conrad 4 DNA Vaccines: Experiences in the Swine Model . 49 Francesc Accensi , Fernando Rodríguez , and Paula L. Monteagudo 5 Novel Adjuvants and Immunomodulators for Veterinary Vaccines . 63 Peter M.H. Heegaard , Yongxiang Fang , and Gregers Jungersen 6 Polymerase Mechanism-Based Method of Viral Attenuation . 83 Cheri A. Lee , Avery August , Jamie J. Arnold , and Craig E. Cameron 7 BacMam Platform for Vaccine Antigen Delivery . 105 Günther M. Keil , Reiko Pollin , Claudia Müller , Katrin Giesow , and Horst Schirrmeier 8 Laboratory-Scale Production of Replication-Deficient Adenovirus Vectored Vaccines . 121 Susan J. Morris , Alison V. Turner , Nicola Green , and George M. Warimwe 9 Generation of Recombinant Modified Vaccinia Virus Ankara Encoding VP2, NS1, and VP7 Proteins of Bluetongue Virus. 137 Alejandro Marín- López and Javier Ortego 10 Generation of Recombinant Capripoxvirus Vectors for Vaccines and Gene Knockout Function Studies . 151 Hani Boshra , Jingxin Cao , and Shawn Babiuk 11 Recombinant Swinepox Virus for Veterinary Vaccine Development . 163 Hong-Jie Fan and Hui-Xing Lin 12 Generation and Selection of Orf Virus (ORFV) Recombinants . 177 Hanns-Joachim Rziha , Jörg Rohde , and Ralf Amann vii viii Contents 13 Polycistronic Herpesvirus Amplicon Vectors for Veterinary Vaccine Development . 201 Anita Felicitas Meier , Andrea Sara Laimbacher , and Mathias Ackermann 14 Construction and Application of Newcastle Disease Virus- Based Vector Vaccines . 225 Paul J. Wichgers Schreur 15 Chimeric Pestivirus Experimental Vaccines . 239 Ilona Reimann , Sandra Blome , and Martin Beer 16 Analysis of the Cellular Immune Responses to Vaccines . 247 Nicholas Svitek , Evans L. N. Taracha , Rosemary Saya , Elias Awino , Vishvanath Nene , and Lucilla Steinaa Index . 263 Contributors FRANCESC ACCENSI • Centre de Recerca en Sanitat Animal (CReSA) , Bellaterra , Barcelona , Spain ; Departament de Sanitat i d’Anatomia Animals, Universitat Autònoma de Barcelona (UAB) , Bellaterra, Barcelona , Spain MATHIAS ACKERMANN • Vetsuisse Faculty, Institute of Virology , University of Zurich , Zurich , Switzerland RALF AMANN • Institute of Immunology , Friedrich-Loeffl er-Institute , Greifswald, Island of Riems , Germany; Department of Immunology, Interfaculty Institute of Cell Biology, Eberhard Karls Universität, Tübingen, Germany JAMIE J. ARNOLD • Department of Biochemistry and Molecular Biology, The Pennsylvania State University , University Park , PA , USA AVERY AUGUST • Department of Microbiology and Immunology, College of Veterinary Medicine , Cornell University , Ithaca , NY , USA ELIAS AWINO • Vaccine Biosciences, International Livestock Research Institute , Nairobi , Kenya SHAWN BABIUK • Canadian Food Inspection Agency , Winnipeg , MB , Canada NATALIA BARREIRO-PIÑEIRO • Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS) , University of Santiago de Compostela , Santiago de Compostela , Spain MARTIN BEER • Institute of Diagnostic Virology , Friedrich-Loeffl er-Institut , Greifswald, Insel Riems , Germany JAVIER BENAVENTE • Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS) , University of Santiago de Compostela , Santiago de Compostela , Spain SANDRA BLOME • Institute of Diagnostic Virology , Friedrich-Loeffl er-Institut , Greifswald, Insel Riems , Germany HANI BOSHRA • Canadian Food Inspection Agency , Winnipeg , MB , Canada ALBERTO BRANDARIZ-NÚÑEZ • Department of Biochemistry and Molecular Biology, Centro Singular de Investigación en Química Biológica y Materiales Moleculares (CIQUS) , University of Santiago de Compostela , Santiago de Compostela , Spain ; Department of Biochemistry, Center for Biophysics and Computational Biology, University of Illinois at Urbana-Champaign , Urbana , IL , USA ALEJANDRO BRUN • Centro de Investigación en Sanidad Animal (CISA) , Instituto Nacional de Investigación y Tecnología Agraria y Almentaria (INIA) , Valdeolmos , Madrid , Spain CRAIG E. CAMERON • Department of Biochemistry and Molecular Biology, The Pennsylvania State University , University Park , PA , USA JINXING CAO • Public Health Agency of Canada , Winnipeg , MB , Canada UDO CONRAD • Department of Molecular Genetics , Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) , Gatersleben , Germany ix x Contributors HONG-JIE FAN • College of Veterinary Medicine , Nanjing Agricultural University , Nanjing , China YONGXIANG FANG • Section for Immunology and Vaccinology, National Veterinary Institute, Technical University of Denmark , Frederiksberg C , Denmark ; State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Public Health of Ministry of Agriculture , Lanzhou Veterinary Research Institute, CAAS , Lanzhou , China KATRIN GIESOW • Institut für Molekulare Virologie und Zellbiologie und Institut für Virusdiagnostik , Friedrich-Loeffl er-Institut , Greifswald, Insel Riems, Germany; Institut für VirusdiagnostikFriedrich-Loeffl er-Institut, Greifswald, Insel Riems, Germany NICOLA GREEN • The Clinical Biomanufacturing Facility , University of Oxford , Oxford , UK PETER M. H. HEEGAARD • Section for Immunology and Vaccinology, National Veterinary Institute , Technical University of Denmark , Frederiksberg C , Denmark GREGERS JUNGERSEN • Section for Immunology and Vaccinology, National Veterinary Institute , Technical University of Denmark , Frederiksberg