2015 Annual Meeting Abstract Supplement Late-Breaking Abstract Submissions All Late-Breaking Abstracts will be presented on Thursday, March 26, from 8:30 am–12:00 noon. These abstracts will be available via the mobile event app, online planner, and a downloadable PDF from the SOT website. 54th Annual Meeting and ToxExpoTM San Diego, California March 22–26, 2015 www.toxicology.org THURSDAY POSTER SESSION MAP March 2015—8:30 AM to 12:00 Noon—Sails Pavilion Poster Set Up—7:00 AM to 8:30 AM 260 259 258 257 256 301 302 303 304 305 660 659 658 657 656 251 252 253 254 255 310 309 308 307 306 651 652 653 654 655 250 249 248 247 246 311 312 313 314 315 650 649 648 647 646 241 242 243 244 245 320 319 318 317 316 641 642 643 644 645 240 239 238 237 236 321 322 323 324 325 640 639 638 637 636 231 232 233 234 235 330 329 328 327 326 631 632 633 634 635 230 229 228 227 226 331 332 333 334 335 630 629 628 627 626 221 222 223 224 225 340 339 338 337 336 621 622 623 624 625 220 219 218 217 216 341 342 343 344 345 620 619 618 617 616 211 212 213 214 215 350 349 348 347 346 611 612 613 614 615 210 209 208 207 206 351 352 353 354 355 610 609 608 607 606 201 202 203 204 205 360 359 358 357 356 601 602 603 604 605 170 169 168 167 166 401 402 403 404 405 570 569 568 567 566 161 162 163 164 165 410 409 408 407 406 561 562 563 564 565 160 159 158 157 156 411 412 413 414 415 560 559 558 557 556 151 152 153 154 155 420 419 418 417 416 551 552 553 554 555 150 149 148 147 146 421 422 423 424 425 550 549 548 547 546 141 142 143 144 145 430 429 428 427 426 541 542 543 544 545 140 139 138 137 136 431 432 433 434 435 540 539 538 537 536 131 132 133 134 135 440 439 438 437 436 531 532 533 534 535 130 129 128 127 126 441 442 443 444 445 530 529 528 527 526 121 122 123 124 125 450 449 448 447 446 521 522 523 524 525 120 119 118 117 116 451 452 453 454 455 520 519 518 517 516 111 112 113 114 115 460 459 458 457 456 511 512 513 514 515 110 109 108 107 106 510 509 508 507 506 101 102 103 104 105 501 502 503 504 505 ENTRANCE Photography in all poster sessions is prohibited without the consent of the poster presenter(s)/author(s). Please respect your colleagues' rights to privacy. Thursday, March 26, Poster Session by Location Session Title Abstract #s Poster Board #s Late-Breaking Poster Session 1 Alternatives to Mammalian Models and Animal Models 2469-2498 101-130 Chemical & Biological Weapons 2499-2507 131-139 Ecotoxicology, Food Safety, and Natural Products 2508-2525 140-157 Metals 2526-2537 158-169 Persistent Organic Pollutants (POPs) 2538 170 Late-Breaking Poster Session 2 Cardiovascular Toxicology/Hemodynamics, Inhalants, Cardiopulmonary 2539-2557 201-219 Kidney and Liver 2558-2576 220-238 Cell Death/Apoptosis 2577-2582 239-244 Epigenetics 2583-2584 245-246 Oxidative Injury and Redox Biology, and Receptors 2585-2591 247-253 Carcinogenesis 2592-2595 254-257 Late-Breaking Poster Session 3 Regulation/Policy 2596 301 Risk Assessment 2597-2615 302-320 Safety Assessment: Nonpharmaceutical 2616-2621 321-326 Safety Assessment: Drug Development and Discovery 2622-2635 327-340 Clinical and Translational Toxicology 2636-2638 341-343 Computational Toxicology and Bioinformatics 2639-2648 344-353 Mixtures 2649-2653 354-358 Education, Ethical, Legal, and Social Issues 2654 359 Late-Breaking Poster Session 4 Children’s Health/Juvenile Toxicity 2655-2661 401-407 Developmental Basis of Adult Disease and Developmental Toxicology 2662-2675 408-421 Endocrine Toxicology and Reproductive Toxicology 2676-2690 422-436 Nanotoxicology 2691-2704 437-450 Systems Biology and Toxicology 2705-2711 451-457 Late-Breaking Poster Session 5 Neurotoxicity and Neurodegenerative Disease 2712-2747 501-536 Pesticides 2748-2749 537-538 Immunotoxicity and Autoimmunity/Hypersensitivity 2750-2756 539-545 Inflammation and Disease, Methods and Mechanisms 2757-2763 546-552 Gene Regulation/Signal Transduction/Genotoxicity and DNA Repair 2764-2774 553-563 Skin 2775-2778 564-567 Medical Devices 2779-2781 568-570 Late-Breaking Poster Session 6 Biomarkers 2782-2801 601-620 Exposure Assessment/Biomonitoring 2802-2815 621-634 Biotransformation/Cytochrome P450 2816-2820 635-639 Disposition/Pharmacokinetics and Pharmacogenomics/Genetic Polymorphisms 2821-2825 640-644 Biological Modeling 2826-2829 645-648 Epidemiology 2830-2831 649-650 Stem Cell Biology and Toxicology 2832-2840 651-659 1 Thursday, March 26, Poster Session by Topic TOPIC ABSTRACT #s POSTER Session Title BOARD #s Alternatives to Mammalian Models and Animal Models 2469-2498 101-130 Late-Breaking Poster Session 1 Biological Modeling 2826-2829 645-648 Late-Breaking Poster Session 6 Biomarkers 2782-2801 601-620 Late-Breaking Poster Session 6 Biotransformation/Cytochrome P450 2816-2820 635-639 Late-Breaking Poster Session 6 Carcinogenesis 2592-2595 254-257 Late-Breaking Poster Session 2 Cardiovascular Toxicology/Hemodynamics, Inhalants, 2539-2557 201-219 Late-Breaking Poster Session 2 Cardiopulmonary Cell Death/Apoptosis 2577-2582 239-244 Late-Breaking Poster Session 2 Chemical & Biological Weapons 2499-2507 131-139 Late-Breaking Poster Session 1 Children’s Health/Juvenile Toxicity 2655-2661 401-407 Late-Breaking Poster Session 4 Clinical and Translational Toxicology 2636-2638 341-343 Late-Breaking Poster Session 3 Computational Toxicology and Bioinformatics 2639-2648 344-353 Late-Breaking Poster Session 3 Developmental Basis of Adult Disease and Developmental 2662-2675 408-421 Late-Breaking Poster Session 4 Toxicology Disposition/Pharmacokinetics and 2821-2825 640-644 Late-Breaking Poster Session 6 Pharmacogenomics/Genetic Polymorphisms Ecotoxicology, Food Safety, and Natural Products 2508-2525 140-157 Late-Breaking Poster Session 1 Education, Ethical, Legal, and Social Issues 2654 359 Late-Breaking Poster Session 3 Endocrine Toxicology and Reproductive Toxicology 2676-2690 422-436 Late-Breaking Poster Session 4 Epidemiology 2830-2831 649-650 Late-Breaking Poster Session 6 Epigenetics 2583-2584 245-246 Late-Breaking Poster Session 2 Exposure Assessment/Biomonitoring 2802-2815 621-634 Late-Breaking Poster Session 6 Gene Regulation/Signal Transduction/Genotoxicity and 2764-2774 553-563 Late-Breaking Poster Session 5 DNA Repair Immunotoxicity and Autoimmunity/Hypersensitivity 2750-2756 539-545 Late-Breaking Poster Session 5 Inflammation and Disease, Methods and Mechanisms 2757-2763 546-552 Late-Breaking Poster Session 5 Kidney and Liver 2558-2576 220-238 Late-Breaking Poster Session 2 Medical Devices 2779-2781 568-570 Late-Breaking Poster Session 5 Metals 2526-2537 158-169 Late-Breaking Poster Session 1 Mixtures 2649-2653 354-358 Late-Breaking Poster Session 3 Nanotoxicology 2691-2704 437-450 Late-Breaking Poster Session 4 Neurotoxicity and Neurodegenerative Disease 2712-2747 501-536 Late-Breaking Poster Session 5 Oxidative Injury and Redox Biology, and Receptors 2585-2591 247-253 Late-Breaking Poster Session 2 Persistent Organic Pollutants (POPs) 2538 170 Late-Breaking Poster Session 1 Pesticides 2748-2749 537-538 Late-Breaking Poster Session 5 Regulation/Policy 2596 301 Late-Breaking Poster Session 3 Risk Assessment 2597-2615 302-320 Late-Breaking Poster Session 3 Safety Assessment: Drug Development and Discovery 2622-2635 327-340 Late-Breaking Poster Session 3 Safety Assessment: Nonpharmaceutical 2616-2621 321-326 Late-Breaking Poster Session 3 Skin 2775-2778 564-567 Late-Breaking Poster Session 5 Stem Cell Biology and Toxicology 2832-2840 651-659 Late-Breaking Poster Session 6 Systems Biology and Toxicology 2705-2711 451-457 Late-Breaking Poster Session 4 2 2015 Society of Toxicology Annual Meeting Late‐Breaking Abstracts ABSTRACT FINAL ID: 2469 Poster Board -101 TITLE: Human Microvascular 3-D Spheroid Model for Toxicity Testing AUTHORS (FIRST INITIAL, LAST NAME): J. K. Das1, Q. H. Felty1 INSTITUTIONS (ALL): 1. Environmental & Occupational Health, Florida International University, Miami, FL, United States. KEYWORDS: Human Vascular Spheroids, Proliferative Lesions, ID3 ABSTRACT BODY: Proliferative microvascular lesions that result from a focal budding of endothelial cells are an aggressive endothelial phenotype that may be modified by exposure to persistent environmental pollutants. The goal of this study was to establish a “Vascular Sphere in Dish model” for toxicity screening based on human vascular cells for the purpose of creating a biologically relevant in vitro model. We developed a method for preparing a 3-dimensional (3-D) vascular spheroid model that consisted of combining human microvascular endothelial cells, human vascular smooth muscle cells, and human fibroblasts. Our procedure cultured the combination of cells in specified ratios in an ultra-low attachment culture dish in B27 media to form the vascular spheres. Next, these spheres were transferred into a dish and cultured for up to 28 days without B27 supplement in a defined fibrin matrix seeded with fibroblasts and maintained in a culture medium comprised of DMEM/F12 and growth supplements. Our data showed that human vascular spheres imitate proliferation and tube formation in vitro. The ability of microvascular endothelial cells to sprout and form stable vascular networks in fibrin matrices was also investigated as a function of vascular stemness. We have previously studied the inhibitor of DNA binding and differentiation protein 3 (Id3). Under defined cell culture conditions, endothelial spheroids that overexpressed ID3 showed an increase in stem cell markers CD34 and VEGFR3; and formed a 3-D vascular tissue that morphologically resembled glomeruloid microvascular lesions.