BETTER SCIENCE, BETTER FISH, BETTER LIFE PROCEEDINGS OF THE NINTH INTERNATIONAL SYMPOSIUM ON TILAPIA IN AQUACULTURE Editors Liu Liping and Kevin Fitzsimmons Shanghai Ocean University, Shanghai, China 22-24 April 2011 Published by the AquaFish Collaborative Research Support Program AquaFish CRSP is funded in part by United States Agency for International Development (USAID) Cooperative Agreement No. EPP-A-00-06-00012-00 and by US and Host Country partners. ISBN 978-1-888807-19-6 1 Dedication: These proceedings are dedicated in honor Of our dear friend Yang Yi It was Dr. Yang Yi who first suggested having this ISTA at Shanghai Ocean University to celebrate SHOU’s move to the new Lingang Campus. It was through his hard work and constant attention with his many friends and colleagues that the entire 9AFAF and ISTA9 came together, despite the terrible illness that eventually took his life at such a young age. Acknowledgements: The editors wish to thank the many people who contributed to the collection and review and editing of these proceedings, especially Mary Riina, Pamila Ramotar, Sidrotun Naim and Zhou TingTing 2 Table of Contents Page KEYNOTE ADDRESS WHY TILAPIA IS BECOMING THE MOST IMPORTANT FOOD FISH ON THE PLANET Kevin Fitzsimmons, Rafael Martinez-Garcia and Pablo Gonzalez-Alanis 9 SECTION I. HEALTH and DISEASE LIVE ATTENUATED BACTERIAL VACCINES IN AQUACULTURE 20 Phillip Klesius and Julia Pridgeon ISOLATION AND CHARACTERIZATION OF Streptococcus agalactiae FROM RED TILAPIA 30 CULTURED IN THE MEKONG DELTA OF VIETNAM Dang Thi Hoang Oanh and Nguyen Thanh Phuong ECO-PHYSIOLOGICAL IMPACT OF COMMERCIAL PETROLEUM FUELS ON NILE TILAPIA, 31 Oreochromis niloticus (L.) Safaa M. Sharaf and Mohsen Abdel-Tawwab ACUTE TOXICITY OF WATER-BORN ZINC IN NILE TILAPIA, Oreochromis niloticus (L.) 44 FINGERLINGS Mohsen Abdel-Tawwab*, Gamal O. El-Sayed, and Sherien H.H.H. Shady FIVE STAR CERTIFICATION PROGRAM AGAINST OFF-FLAVOR IN TILAPIA FILLETS 51 Tomi HONG ACUTE TOXICITY OF AQUEOUS Morinda lucida LEAF EXTRACTS TO NILE TILAPIA, 52 Oreochromis niloticus (LINNAEUS 1857) Oyedapo FAGBENRO and Iyabo AKINDUYITE HAEMATOLOGICAL RESPONSE OF NILE TILAPIA (Oreochromis niloticus) JUVENILES 60 EXPOSED TO TOBACCO (Nicotiana tobaccum) LEAF DUST M.O. OLUFAYO AND I.A. JATTO COMPARATIVE ASSESSMENT OF PARASITE INFESTATION OF TILAPIA IN NATURAL AND 65 CULTURED ENVIRONMENTS ABIDEMI-IROMINI A.O and R.N EZE OXYTETRACYCLINE MARKING STUDIES OF TILAPIA Oreochromis niloticus 70 Yasser Mohammed ABDEL-HADI 3 SECTION II. ACCELERATING AQUACULTURE DEVELOPMENT IN POORER COUNTRIES INTENSITY OF FRESHWATER USE FOR AQUACULTURE IN DIFFERENT COUNTRIES 80 Claude E. BOYD* and LI Li IMPACTS OF THE INTRODUCTION OF ALIEN TILAPIAS (Oreochromis spp.) ON 88 THE FISHERIES AND BIODIVERSITY OF INDIGENOUS SPECIES IN TRI AN RESERVOIR, VIETNAM Le Thanh Hung, Vu Cam Luong, Nguyen Phu Hoa, James Diana DURATION OF APPETITE INHIBITION PREDICTS SOCIAL DOMINANCE IN NILE TILAPIA, 101 Oreochromis niloticus L. Emmanuel M. Vera Cruz, Madelin B. Valdez, Remedios B. Bolivar, and Russell J. Borski FISHMEAL-FREE DIETS IMPROVE THE COST EFFECTIVENESS OF CULTURING NILE 111 TILAPIA (Oreochromis niloticus L.) IN PONDS UNDER AN ALTERNATE DAY FEEDING STRATEGY Russell J. Borski, Remedios B. Bolivar, Eddie Boy T. Jimenez, Roberto Miguel V. Sayco, Reginor Lyzza B. Arueza, Charles R. Stark, and Peter R. Ferket HEAT-INDUCED GERM CELL LOSS IN SUB-ADULT NILE TILAPIA Oreochromis niloticus 119 Narayan P. Pandit, Madhav K. Shrestha and Masaru Nakamura EFFECTS OF STOCKING DENSITY ON THE GROWTH, SURVIVAL AND YIELD 120 PERFORMANCE OF NILE TILAPIA (Oreochromis niloticus, Linn. 1858) IN AN INTEGRATED CAGE-CUM-POND CULTURE SYSTEM C. C. Ngugi, G. Kuria, K. Quagrainie, and S. Macharia FOOD SAFETY STUDY OF LEAFY GREENS IRRIGATED WITH TILAPIA FARM 121 EFFLUENTS IN TAMAULIPAS P. González-Alanis* J. I. Gutierrez-Olguín, H. Ezqueda-Palacios, H. H. Gojon-Báez, G. Aguirre-Guzmán, F. M. Guzmán-Saénz, K. M. Fitzsimmons. MASCULINIZATION OF NILE TILAPIA (Oreochromis niloticus L.) USING LYOPHILIZED 123 TESTES FROM CARABAO (Bubalus bubalis carabanesis L.), BULL (Bos indicus L.) AND BOAR (Sus domesticus L.) Ramjie Y. Odin and Remedios B. Bolivar POTENTIAL USE OF BACTERIAL DEGRADATION TO ELIMINATE 142 METHYLTESTOSTERONE FROM INTESIVE TILAPIA MASCULINIZATION SYSTEMS Rosa M. Padrón-López, Lucero Vázquez-Cruz, Ulises Hernández-Vidal, W. M. Contreras-Sánchez* and K. Fitzsimmons HOW TO PRODUCE BILLIONS OF HIGH QUALITY TILAPIA FRY 144 Ram C. Bhujel IMPROVING THE SUPPLY CHAIN OF TILAPIA INDUSTRY IN THE PHILIPPINES 153 Wilfred E. Jamandre, Upton Hatch, Remedios B. Bolivar, Russell Borski 4 DEVELOPMENT OF SUSTAINABLE AQUACULTURE PRACTICES IN TABASCO, 175 MEXICO USING NOVEL IAA TECHNOLOGY R. Martínez- García*, M. F. Cifuentes-Alonso, M. A. Estrada Botello, A. S. Lopez Torres, M. de Jesús Contreras-García, A. Macdonal-Vera, E. González-Arévalo, W. M. Contreras-Sánchez, K. Fitzsimmons CONSTRAINTS AND OPPORTUNITIES IN CAGE AQUACULTURE IN GHANA 182 Gifty Anane-Taabeah, Emmanuel A. Frimpong, Stephen Amisah, and Nelson Agbo GEOSPATIAL MODELING OF SITE SUITABILITY FOR POND BASED TILAPIA AND 191 CLARIAS FARMING IN UGANDA Herbert Ssegane, E.W.Tollner, and Karen Veverica WHAT INFLUENCES THE SUCCESS OF AQUACULTURAL RESEARCH PROJECTS? 192 Steven Buccola, Lin Qin and Rolf Fare METHODS FOR ASSESSING ECONOMIC, ENVIRONMENTAL AND SOCIAL IMPACTS 200 OF AQUACULTURE TECHNOLOGIES: ADOPTION OF INTEGRATED AGRICULTURE-AQUACULTURE IN MALAWI John Antle and Roberto Valdivia VALUE CHAIN OF CULTURED SNAKEHEAD FISH IN THE MEKONG DELTA 211 Le Xuan Sinh*, Robert S. Pomeroy & Do Minh Chung USE OF GONADOTROPIN RELEASING HORMONE ANALOGS ON THE INDUCED 214 REPRODUCTION OF CHAME Dormitator latifrons Gustavo A. Rodriguez M. de O.*, Eva A. Medina H., Jeniffer Velazquez S., Vanesa Lopez L., Cristobal Roman R., Konrad Dabrowski, Eladio Gaxiola Camacho, Maria C. Haws SECTION III. GENETICS and REPRODUCTION IMPROVING SALINITY TOLERANCE IN TILAPIAS: PAST EXPERIENCE AND FUTURE 221 PROSPECTS Avner CNAANI, Ariel VELAN, Gideon HULATA* COMPARISON BETWEEN GREEN WATER AND CLEAR WATER SYSTEMS DURING THE 231 MASCULINIZATION PROCESS OF SILVER TILAPIA, Oreochromis niloticus Ryan S. Mohammed and Indar W. Ramnarine OSMOREGULATORY CAPACITY OF THE NILE TILAPIA (Oreochromis niloticus (L. )) 232 DURING EARLY LIFE STAGES. Fridman, S., Bron, J.E. and Rana, K.J. TILAPIA GERMPLASM IN CHINA: CHANCE AND CHALLENGE 249 Zhao Jinliang EFFECTS OF Aloe vera (Liliaceae) ON THE GONAD DEVELOPMENT IN NILE TILAPIA, 255 Oreochromis niloticus (Linnaeus 1758) Temitope JEGEDE 5 MORPHOMETRIC AND MERISTIC CHARACTERISTICS AND THEIR VARIATIONS 262 BETWEEN TWO DIFFERENT STRAINS (GIFT & GIFU) OF NILE TILAPIA, Oreochromis niloticus (Linnaeus, 1758) K. M. Shahriar Nazrul GENETIC STOCK IMPROVEMENT OF THE GIFT STRAIN IN BANGLADESH 269 M.G. Hussain, A.H.M. Kohinoor, N.H. Nguyen and R.W. Ponzoni PRODUCTIVE PERFORMANCE AND MUSCLE GROWTH OF THREE DIFFERENT 277 STRAINS OF NILE TILAPIA, Oreochromis niloticus, DURING THE INITIAL DEVELOPMENT Thiago M. de Freitas, Juliana T. Kojima, Natalia de J. Leitão, Caroline Nebo, Fernanda Carani, Maeli Dal Pai-Silva and Maria Célia Portella* SECTION IV. NUTRITION and FEEDS EFFECTS OF SAPONIN FRACTIONS FROM Trigonella foenum-graecum AND 279 Balanites aegyptiaca ON GENE EXPRESSION OF GH, IGF-1 AND THEIR RESPECTIVE RECEPTORS, GROWTH, NUTRIENT UTILIZATION, BODY COMPOSITION OXYGEN CONSUMPTION AND PLASMA IGF-1 IN NILE TILAPIA (Oreochromis niloticus, L.). T. Stadtlander, W. K. B. Khalil,, B. Levavi-Sivan, H. Dweik5, M. Qutob, S. Abu-Lafi, Z. Kerem and U. Focken, K. Becker BROODSTOCK DIETS WITH ADDED CRUDE PALM OIL RESULTED IN IMPROVED 296 REPRODUCTIVE PERFORMANCE, EGG HATCHABILITY AND LARVAL QUALITY OF NILE TILAPIA Oreochromis niloticus Wing-Keong Ng and Yan Wang DISTILLERS DRIED GRAINS WITH SOLUBLES AS ALTERNATIVE PROTEIN SOURCES IN 297 DIETS OF TILAPIA, Oreochromis niloticus LIM, Chhorn, Erchao LI and Phillip H. KLESIUS ECONOMICALLY FEASIBLE FISH FEED FOR GIFT TILAPIA (Oreochromis niloticus) FOOD 298 FISH CULTURE IN SRI LANKA M.H.S. Ariyaratne SUPPLEMENTAL FEEDING OF NILE TILAPIA (Oreochromis niloticus L.) IN FERTILIZED 305 PONDS USING COMBINED FEED REDUCTION STRATEGIES R. B. Bolivar, E. Boy T. Jimenez, R. Miguel V. Sayco, and R. J. Borski THE USE OF ROASTED COFFEE PULP AS A FEED SUPPLEMENT IN PRACTICAL DIETS 313 FOR NILE TILAPIA, Oreochromis niloticus (L.) Mohsen ABDEL-TAWWAB PARTIAL AND TOTAL REPLACEMENT OF FISHMEAL WITH CHEESE PROCESSING 322 BY-PRODUCT MEAL IN PRACTICAL DIETS FOR NILE TILAPIA, Oreochromis niloticus (L.): A PRELIMINARY STUDY Mohsen ABDEL-TAWWAB*, Fayza E. ABBASS, and Medhat E.A. SEDEN 6 SECTION V. ECONOMICS and COUNTRY - REGIONAL REPORTS TILAPIA CULTURE IN TRINIDAD AND TOBAGO: YET ANOTHER UPDATE 332 Indar W. Ramnarine and Capildeo Barrath 60 YEARS OF TILAPIA AQUACULTURE IN NIGERIA 337 O. A. FAGBENRO, O. S. FASASI, T. JEGEDE and O. O. OLAWUSI-PETERS BEST AQUACULTURE PRACTICES STANDARDS FOR THE TILAPIA INDUSTRY 348 Darryl JORY A HANDS-ON TRAINING HELPED PROLIFERATION OF TILAPIA CULTURE 349 IN BANGLADESH BAQUI*, M. A. AND BHUJEL, R. C. STATUS AND SUSTAINABILITY ANALYSIS OF THE TILAPIA AQUACULTURE IN CHINA 361 LIU Liping*, ZHANG Wenbo, Francis MURRAY, David LITTLE TILAPIA: THE SEARCH FOR A SUSTAINABLE MODEL TO BALANCE BETWEEN 362 ENVIRONMENT, PEOPLE AND ECONOMY. SNIR, Israel and SNIR, Yedod TILAPIA - THE HISTORICAL PROMISE FOR TODAY, SOCIAL JUSTICE AND SECURITY 364 SNIR, Israel and SNIR, Yedod SECTION VI. GROWOUT SYSTEMS THE INTERNATIONAL TILAPIA AND AQUAPONICS COURSE AT THE UNIVERSITY 367 OF THE VIRGIN