COLLEGEWIDE COURSE OUTLINE OF RECORD MEDL, 206 HEMATOLOGY TECHNIQUES II COURSE TITLE: Hematology Techniques II COURSE NUMBER: MEDL 206 PREREQUISITE: MEDL 205 Hematology Techniques I and Program Advisor Approval SCHOOL: Health Sciences PROGRAM: Medical Laboratory Technology CREDIT HOURS: 3 CONTACT HOURS: Lecture: 2 Lab: 2 DATE OF LAST REVISION: Spring, 2014 EFFECTIVE DATE OF THIS REVISION: Fall, 2014 CATALOG DESCRIPTION: This course continues the study of principles and procedures in hematology. It introduces procedures which lie outside those routinely performed. Continues cell differentiation, with emphasis upon early and less commonly encountered abnormal cells, with associated special stains. Includes clinicopathologic correlations. MAJOR COURSE LEARNING OBJECTIVES: Upon completion of the course, the student will be expected to: 1. Identify and describe morphologic alterations associated with WBC and RBC dyscrasia and their clinical importance. 2. Describe proper specimen collection, set-up and evaluation for bone tomorrows and spinal fluids. 3. Explain theoretical and practical application of cytochemistry for cell differentiation. COURSE CONTENT: Topical areas of study will include – 1. Understand the clinical significance of and morphologic alterations associated with WBC dyscrasia. 1.1 Differentiate microscopically between normal and abnormal white blood cells. 1.2 Diagram and explain how toxic changes alter morphology. 1.3 Diagram and explain morphologic changes associated with Alder-Reilly anomaly, Pelger-Huet anomaly, and Chediak Higashi syndrome. 1.4 Define terms and list possible causes for the states of neutrophilia, neutropenia, eosinophilia, eosinopenia, basophilia, lymphocytosis, and monocytosis. 2. Know and understand the classification neoplastic diseases. 2.1 Define the term leukemia. 2.2 Define the terms acute, subacute, and chronic as related to duration of disease. 2.3 Define the terms leukemic, subleukemic, and aleukemic as related to the numbers of white cells in the peripheral blood. Ivy Tech Community College 1 MEDL 206 Academic Affairs 2.4 Classify leukemia’s as to cell type involved (i.e. lymphocytic) and what cells are seen on the peripheral smear. 2.5 Define the term lymphoma and name a disease that fits that classification. 2.6 Define the disease characteristics of multiple myeloma. 3. The clinical significance and morphologic alterations associated with hemoglobinopathies. 3.1 Describe four abnormal hemoglobins associated with defective globin structure. 3.2 Give examples of pathologic states due to each of the abnormal hemoglobins. 3.3 Explain expected laboratory results for the pathologic states due to each of the abnormal hemoglobin’s. 3.4 State the principle of hemoglobin electrophoresis. 3.5 Describe migration locations of hemoglobins during electrophoresis. 3.6 Describe the screening test principle and procedure for detecting sickle cell disease. 4. Clinical significance and morphologic alterations associated with RBC dyscrasia. 4.1 Define terms for difference in erythrocyte size, shape and content. 4.2 Differentiate between normal and abnormal red cells under the microscope. 4.3 List six (6) red blood cell dyscrasia and describe abnormal red cell morphology associated with each. 4.4 Classify anemias according to morphology and cause. 4.5 List laboratory procedures as appropriate for identification of red cell dyscrasias. 4.6 State principle of red cell fragility test. 4.7 Compare values of red cells fragility tests to red cell morphology. 4.8 List normal values for red cell fragility test as stated by instructor. 5. The importance and use of the differential as an aid in clinical diagnosis. 5.1 Identify and classify RBC's by morphology on Wright-stained smears. 5.2 Identify and classify WBC's by morphology on a Wright stained smears. 5.3 Perform a platelet estimate on Wright-stained smears and designate as normal, increased or decreased. 5.4 Describe and perform a white cell estimate on Wright-stained smears. 5.5 Perform complete differential counts. 5.6 Perform complete blood counts (CBC). 6. Recognize the importance of proper specimen collection and set-up. 6.1 List sites used for bone marrow aspiration. 6.2 Describe the technique for processing bone marrow aspirates. 6.3 Differentiate those leukocytes found normally in peripheral blood from those found in bone marrow. 7. Theoretical and practical application of cytochemistry for cell differentiation. 7.1 State the principle and use for the peroxidase stain. 7.2 State the use for the periodic acid-Schiff stain. (PAS). Ivy Tech Community College 2 MEDL 206 Academic Affairs 7.3 State the principle and use for the Sudan black stain. 7.4 State the principle and use for the leukocyte alkaline phosphates stain. (L.A.P.) 7.5 State the use for the acid phosphates stain. 7.6 State the use for the two-esterase stains. HOW TO ACCESS THE IVY TECH COMMUNITY COLLEGE LIBRARY: The Ivy Tech Library is available to students’ on- and off-campus, offering full text journals and books and other resources essential for course assignments. Go to http://www.ivytech.edu/library/ and choose the link for your campus. ACADEMIC HONESTY STATEMENT: The College is committed to academic integrity in all its practices. The faculty value intellectual integrity and a high standard of academic conduct. Activities that violate academic integrity undermine the quality and diminish the value of educational achievement. Cheating on papers, tests or other academic works is a violation of College rules. No student shall engage in behavior that, in the judgment of the instructor of the class, may be construed as cheating. This may include, but is not limited to, plagiarism or other forms of academic dishonesty such as the acquisition without permission of tests or other academic materials and/or distribution of these materials and other academic work. This includes students who aid and abet as well as those who attempt such behavior. COPYRIGHT STATEMENT: Students shall adhere to the laws governing the use of copyrighted materials. They must insure that their activities comply with fair use and in no way infringe on the copyright or other proprietary rights of others and that the materials used and developed at Ivy Tech Community College contain nothing unlawful, unethical, or libelous and do not constitute any violation of any right of privacy. ADA STATEMENT: Ivy Tech Community College seeks to provide reasonable accommodations for qualified individuals with documented disabilities. If you need an accommodation because of a documented disability, please contact the Office of Disability Support Services. If you will require assistance during an emergency evacuation, notify your instructor immediately. Look for evacuation procedures posted in your classroom. Ivy Tech Community College 3 MEDL 206 Academic Affairs .