Workshop Program 11th Workshop on Cyanobacteria Washington University in St. Louis August 7 – 11, 2013 Hosted by: 11th Workshop on Cyanobacteria Washington University in St. Louis, MO USA August 7-11, 2013 Welcome to the 11th Workshop on Cyanobacteria! We are very pleased with the great level of interest in this year’s Workshop and with the opportunity to cohost a Joint session with the 2013 Light Harvesting Satellite Meeting. Organizing Committee: Himadri Pakrasi Robert Blankenship Beronda Montgomery Louis Sherman Teresa Thiel Local Organizing Committee: Gayle Geren Chad Henry Kathryn Woerheide Workshop Program Contents 1. Agenda 2. Oral Presentation Abstracts 3. Poster Presentation Abstracts 4. Registered Participants Workshop Sponsors: 11th Workshop on Cyanobacteria Washington University in St. Louis, MO USA August 7-11, 2013 Wednesday, August 7, 2013 1:00-5:00 PM Check-in & Poster Hanging 5:30-6:30 Dinner1 7:00-7:10 Introduction: Himadri Pakrasi Welcome: Holden Thorp, Provost, Washington University2 7:10-7:45 Plenary I: Robert Haselkorn, The University of Chicago: HetR Structure and Metabolism in Anabaena Heterocyst Differentiation (Introduction by Himadri Pakrasi, Washington University)2 7:45-8:00 Meeting Details 8:00-9:00 Mixer3 Thursday, August 8, 2013 7:00-8:15 AM Breakfast4 8:30-9:50 GenomiCs, BioinformatiCs, and Diversity (Chair: Rajeev Aurora, Saint Louis University)2 8:30-8:50 Oded Beja, Technion: Viral-inspired photosynthesis? 8:50-9:10 Christian Beck, Humboldt University Berlin: A daily temporal program for rhythmic expression of protein-coding and non-coding genes in response to light and dark in Synechocystis sp. PCC 6803 9:10-9:30 Linda Vuorijoki, University of Turku: Establishment of high-precision, wide dynamic range proteomics for high-throughput protein quantification in cyanobacterium Synechocystis sp. PCC 6803 9:30-9:50 Wei-Jun Qian, Pacific Northwest National Laboratory: Broad Light-dependent Redox Regulation on Protein Thiols in Cyanobacteria Revealed by Quantitative Site-Specific Proteomics Profiling 9:50-10:15 Break 10:15-10:50 Plenary II: Jonathan Zehr, UC Santa Cruz: Nitrogen-fixing cyanobacterial associations in the sea (Introduction by James Golden, UC San Diego)2 11:00-12:00 Bioengineering in CyanobaCteria I (Chair: Wim Vermaas, Arizona State University)2 11:00-11:20 Daniel Ducat, Michigan State University: Regulation of Photosynthesis via Metabolic Sink Capacity in Sucrose-Secreting Cyanobacteria & Engineered Microbial Autotroph/Heterotroph Consortia 11:20-11:40 Hélder Miranda, Uppsala University: Optimizing cyanobacterial biofuel production 11:40-12:00 Gaozhong Shen, The Pennsylvania State University: Pathway engineering for production and characterization of carotenoids in cyanobacterium Synechococcus sp. PCC 7002: canthaxanthin synthesis, assembly and function 12:00-1:30 PM Lunch4 1:30-3:45 Free Time 3:45-4:00 Group Photo3 4:00-6:00 Poster Session3 6:00-7:15 Dinner1 7:30-8:30 Bioengineering in CyanobaCteria II (Chair: Brian Pfleger, University of Wisconsin- Madison)2 7:30-7:50 Fei Cai, DOE Joint Genome Institute: Analysis of Bacterial Microcompartment Domain- Containing Proteins for Assembly, Chimeric Incorporation, and Selective Permeability in Carboxysomes 7:50-8:10 Justin Ungerer, National Renewable Energy Laboratory: Photobiological Ethylene Production in Synechocystis 6803 8:10-8:30 Pia Lindberg, Uppsala University: Production of isoprenoids in Synechocystis PCC 6803 8:30 Refreshments & Poster Viewing3 Friday, August 9, 2013 7:00-8:15 AM Breakfast4 8:30-9:30 Bioengineering in CyanobaCteria III (Chair: Fuzhong Zhang, Washington University)2 8:30-8:50 Matthew Begemann, University of Wisconsin-Madison: Organic acid tolerance and production in Synechococcus sp. PCC 7002 8:50-9:10 David Welkie, Purdue University: The impact of media adjustments on growth and analysis of carbohydrate storage granules in the diazotrophic cyanobacterium Cyanothece sp. PCC 7822 9:10-9:30 Swathi Alagesan, Indian Institute of Technology Bombay: Metabolic flux analysis of unicellular cyanobacteria under nitrogen fixing and non-fixing mixotrophic condition 9:30-10:00 Break 10:00-10:35 Plenary III: Todd Yeates, UC Los Angeles: Structural insights into the function of carboxysomes and related bacterial microcompartments (Introduction by Robert Burnap, Oklahoma State University)2 11:00-12:00 PhotosynthetiC and Respiratory Metabolism I (Chair: Robert Burnap, Oklahoma State University)2 10:40-11:00 Steven Holland, Oklahoma State University: Redox changes accompanying carbon deprivation in cyanobacterium Synechocystis sp. PCC 6803 11:00-11:20 Jiri Jablonsky, University of South Bohemia in Ceske Budejovice: From genome-scale modeling to multi-scale kinetic model of carbon metabolism for cyanobacteria 11:20-11:40 Tuomas Huokko, University of Turku: Insights into the function of type 2 NAD(P)H dehydrogenases in Synechocystis sp. PCC 6803 11:40-12:00 Hualing Mi, Chinese Academy of Sciences: Biochemical properties of NADPH dehydrogenase complexes from cyanobacteria 12:00-1:30 PM Lunch4 1:30-2:50 PhotosynthetiC and Respiratory Metabolism II (Chair: Wendy Schluchter, University of New Orleans)2 1:30-1:50 Ipsita Dutta, Arizona State University: Biohydrogen production from Synechocystis sp. PCC6803: significance of the diaphorase moiety in hydrogenase formation 1:50-2:10 Ankita Kothari, Arizona State University: Characterization of Bidirectional Hydrogenase Mediated Powerful Hydrogen Production from the Newly Isolated Cyanobacterial Species 2:10-2:30 Jingdong Zhao, Peking University: Phycobilisome Structure and association with photosystem II based on 3D reconstruction using single particle electron microscopy 2:30-2:50 Haijun Liu, Washington University: Phycobilisomes Feed Both Photosystems in One Megacomplex in Synechocystis sp.PCC 6803, a cyanobacterium 2:50-3:00 Break 3:00-4:00 PhotosynthetiC and Respiratory Metabolism III (Chair: Cheryl Kerfeld, UC Berkeley)2 3:00-3:20 Luca Bersanini, University of Turku: Flavodiiron proteins Flv4 and Flv2 co-operate with light- harvesting antenna to enhance PSII photoprotection in Synechocystis sp. PCC6803 3:20-3:40 Marco Agostoni, Michigan State University: Role of cyclic di-GMP signaling in light-dependent responses in cyanobacteria 3:40-4:00 Lara Jazmin, Vanderbilt University: Mapping photoautotrophic metabolism of cyanobacteria by isotopically nonstationary 13C flux analysis 4:00-4:15 Break 4:15-5:15 PhotosynthetiC and Respiratory Metabolism IV (Chair: Tae Seok Moon, Washington University)2 4:15-4:35 Rajib Saha, The Pennsylvania State University: Comparative Genome-Scale Modeling of the Metabolic Potential of Cyanobacteria Cyanothece sp. ATCC 51142 and Synechocystis sp. PCC 6803# 4:35-4:55 Richard Baran, Lawrence Berkeley National Lab: Mapping cyanobacterial metabolism using metabolomics 4:55-5:15 Ralf Steuer, Humboldt University Berlin: Computational models fo cyanobacterial metabolism: Systemic properties of phototrophic growth 5:15-6:30 Transfer to Missouri Botanical Garden 6:30-10:00 Workshop Dinner at Missouri Botanical Garden Saturday, August 10, 2013 7:00-8:15 AM Breakfast4 8:30-9:05 Plenary IV: Masahiko Ikeuchi, The University of Tokyo: Color tuning in cyanobacterial life (Introduction by David Kehoe, Indiana University)2 9:05-10:25 Stress Responses I (Chair: Beronda Montgomery, Michigan State University)2 9:05-9:25 LaDonna Jones, Indiana University: A sulfur-depleted form of phycocyanin gives Fremyella diplosiphon a fitness advantage in low sulfur conditions 9:25-9:45 Sara B. Pereira, Instituto de Biologia Molecular e Celular: Cyanobacterial extracellular polymeric substances (EPS): production, export and interactions with metal cations 9:45-10:05 Yvonne Zilliges, Freie Universität Berlin: The role of glycogen and PHB metabolism in stress responses 10:05-10:25 Christiane Funk, Umeå University: Importance of Deg/HtrA proteases in Synechocystis sp. PCC 6803 10:25-10:50 Break 10:50-11:50 Stress Responses II (Chair: Beronda Montgomery, Michigan State University)2 10:50-11:10 Nir Keren, Hebrew University of Jerusalem: Coordinated Transporter Activity Shapes High Affinity Iron Acquisition in Cyanobacteria 11:10-11:30 Luis López Maury, Institute of Plant Biochemistry and Photosynthesis: Genome wide copper responses in Synechocystis sp. PCC 6803 11:30-11:50 Hans Matthijs, University of Amsterdam: Hydrogen peroxide as a biocide for selective suppression of harmful cyanobacteria 11:50-1:30 PM Lunch4 1:30-3:00 Poster DisCussion I (Led by Julian Eaton-Rye, University of Otago and Himadri Pakrasi, Washington University)2 3:00-3:15 Break 3:15-4:30 Poster DisCussion II (Ferran Garcia-Pichel, Arizona State University and Peter Lindblad, Uppsala University)2 4:30-6:00 Free Time/Poster Viewing3 6:00-7:30 Dinner with Light Harvesting Satellite Meeting Participants1 7:40-9:05 Joint Session w/ Light Harvesting Satellite Meeting5 7:40-7:45 IntroduCtion to Joint Session: Robert Blankenship, Washington University 7:45-8:05 Jack Chidgey, University of Sheffield: A cyanobacterial chlorophyll synthase-HliD-YCF39-YidC complex links chlorophyll and protein biosynthesis 8:05-8:25 Diana Kirilovsky, Institute of Biology and Technology Saclay: Photoprotection by thermal dissipation of excess absorbed energy: The orange carotenoid protein (OCP) and the fluorescence recovery protein (FRP) 8:25-8:45 Michelle Liberton, Washington University: Probing the Consequences of Antenna Truncation in Cyanobacteria 8:45-9:05 Noam Adir, Technion: X-ray crystallographic and cryo-TEM structures of the phycobilisome photosynthetic