OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP15932PU-N Polyclonal Antibody to ARFGAP3 (C-term) - Aff - Purified Alternate names: ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP1 Quantity: 0.1 mg Concentration: 0.5 mg/ml Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which is thought to interact with ADP- ribosylation factor 1 (ARF1). The encoded protein likely promotes hydrolysis of ARF1-bound GTP, which is required for the dissociation of coat proteins from Golgi- derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene. Uniprot ID: Q9NP61 NCBI: NP_055385.3 GeneID: 26286 Host: Goat Immunogen: Peptide from C Terminus of the protein sequence according to NP_055385.3; NP_001135765.1 Genename: ARFGAP3 AA Sequence: C-NGVVTSIQDRYGS Format: State: Liquid Ig fraction Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide Buffer System: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin Applications: Peptide ELISA: Limit dilution 1:8000. Western blot: 1-3 µg/ml. Approx 55-60kDa band seen in mouse brain extracts. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects ARFGAP3 at C-term. Species: Mouse. Other species not tested. Add. Information: Expected from sequence similarity to react with Human, Rat, Dog, and Cow. Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20160219 1 / 2 AP15932PU-N: Polyclonal Antibody to ARFGAP3 (C-term) - Aff - Purified General Readings: Liu X, Zhang C, Xing G, Chen Q, He F. Functional characterization of novel human ARFGAP3. FEBS Lett 2001 Feb 9;490(1-2):79-83 PMID: 11172815 Pictures: TA302737 staining (2µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20160219 2 / 2 Powered by TCPDF (www.tcpdf.org).