Datasheet: AHP1711 Description: RABBIT ANTI MAPKAP1 Specificity: MAPKAP1 Format: Purified Product Type: Polyclonal Antibody Isotype: Polyclonal IgG Quantity: 0.1 mg Product Details Applications This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit www.bio-rad-antibodies.com/protocols. Yes No Not Determined Suggested Dilution Immunohistology - Paraffin (1) 5.0ug/ml Western Blotting 1.0 - 2.0ug/ml Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. (1)This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. Target Species Human Species Cross Reacts with: Mouse, Rat Reactivity N.B. Antibody reactivity and working conditions may vary between species. Product Form Purified IgG - liquid Antiserum Preparation Antisera to Human MAPKAP1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography. Buffer Solution Phosphate buffered saline Preservative 0.02% Sodium Azide (NaN ) Stabilisers 3 Approx. Protein IgG concentration 1.0mg/ml Concentrations Immunogen A peptide corresponding to a 18 amino acid sequence from near the centre of Human MAPKAP1. External Database Links UniProt: Q9BPZ7 Related reagents Page 1 of 3 Entrez Gene: 79109 MAPKAP1 Related reagents Synonyms MIP1, SIN1 Specificity Rabbit anti MAPKAP1 antibody recognizes the human Mitogen activated protein kinase associated protein 1 (MAPKAP1). MAPKAP1 forms and essential component of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation. It is believed to modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation and also inhibits HRAS and KRAS signaling. MAPKAP1 enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. MAPKAP1 is a ubiquitously expressed protein with highest levels being detected in the heart and skeletal muscle. Multiple alternatively spliced isoforms of MAPKAP1 have been identified. Histology Positive Human skeletal muscle Control Tissue Western Blotting AHP1711 detects bands of approximately 37 and 73kDa in human skeletal muscle lysate Further Reading 1. Wullschleger S et al. (2006) TOR signaling in growth and metabolism. Cell 124:471-84 2. Jacinto E, et al (2006) SIN1/MIP1 maintains rictor-mTOR complex integrity and regulates Akt phosphorylation and substrate specificity. Cell 127:125-37 3. Cheng J, et al (2005) Mip1, an MEKK2-interacting protein, controls MEKK2 dimerization and activation. Mol. Cell. Biol. 25:5955-64 4. Schroder W, et al. (2004) Alternative polyadenylation and splicing of mRNAs transcribed from the human Sin1 gene. Gene 339:17-23 Storage Store at +4oC or at -20oC if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life 18 months from date of despatch. Health And Safety Material Safety Datasheet documentation available at: Information Material Safety Datasheet Documentation #10040 available at: https://www.bio-rad-antibodies.com/uploads/MSDS/10040.pdf Regulatory For research purposes only Related Products Recommended Secondary Antibodies Sheep Anti Rabbit IgG (STAR54...) HRP Recommended Useful Reagents HISTAR DETECTION SYSTEM (STAR3000A) Page 2 of 3 ANTIGEN RETRIEVAL BUFFER, pH8.0 (BUF025A) ANTIGEN RETRIEVAL BUFFER, pH8.0 (BUF025C) TidyBlot™ WESTERN BLOT DETECTION REAGENT:HRP (STAR209P) North & South Tel: +1 800 265 7376 Worldwide Tel: +44 (0)1865 852 700 Europe Tel: +49 (0) 89 8090 95 21 America Fax: +1 919 878 3751 Fax: +44 (0)1865 852 739 Fax: +49 (0) 89 8090 95 50 Email: [email protected] Email: [email protected] Email: [email protected] 'M294664:161006' Printed on 15 Jan 2018 © 2018 Bio-Rad Laboratories Inc | Legal | Imprint Page 3 of 3.