Copyright ©2015-2018 Sophion Bioscience A/S This manual covers the product named Qube 384 All rights reserved This manual contains information protected by copyright. No part of this manual may be photocopied or reproduced in any form without prior written consent from Sophion Bioscience A/S. Trademarks Sophion, Qube and QChip are registered trademarks of Sophion Bioscience A/S in one or more countries. All other trademarks are the property of their respective owners. Sophion Bioscience disclaimer Sophion Bioscience A/S, Sophion K.K. and Sophion Bioscience Inc. cannot be held responsible for any damage caused by the use of the Qube system or related software. Sophion Bioscience A/S, Sophion K.K. and Sophion Bioscience Inc. does not guarantee the correctness, accuracy, reliability, performance or otherwise of the Qube system. The user assumes the risk as to the results and the performance of the Qube system. Information provided in this manual may not be up to date. The disclaimer written above may be incomplete and should be considered together with all other agreements made between Sophion Bioscience A/S, Sophion K.K. and Sophion Bioscience Inc. and the users of the system. Manual revision 2.2.10 Sophion Bioscience A/S Baltorpvej 154, 2750 Ballerup – Denmark Telephone: +45 44 60 88 00 Email: [email protected] Sophion Bioscience, Inc. 400 Trade Center Drive, Suite 6900 Woburn, MA 01801 – United States Telephone: +1 877 898 9010 Sophion Bioscience K.K. Incubation on Campus Honjo Waseda #V203 1011, Nishitomitda, Honjo-shi Saitama 367-0035 – Japan Telephone: +81 495 71 8811 Sophion Bioscience A/S Rm1131, No. 968 West Beijing Rd. Shanghai 200041 – China Telephone: +86 21 52037529 Contents Introduction to Qube...........................................................................................6 Scope and purpose .............................................................................................6 How to read this manual .....................................................................................7 Tips, notes and warnings.....................................................................................7 Quick Guide.........................................................................................................8 Things to remember (Qube ABC) ..........................................................................8 Start-up procedures............................................................................................8 Shut-down procedures ........................................................................................8 1. The Qube System..........................................................................................9 1.1 Setup of the System......................................................................................9 1.1.1 Firewall, IP-address and Network Security..................................................9 1.1.2 Configure data backup ...........................................................................10 1.2 The Qube Instrument ..................................................................................10 1.3 Data PC and Controller PC ............................................................................11 1.4 User PC and network connection ...................................................................11 1.5 QChip 384..................................................................................................12 1.5.1 Handling the QChip 384 .........................................................................12 1.6 Qube software ............................................................................................13 1.6.1 Logging on to Qube software ..................................................................13 2. Safety and Warnings ..................................................................................14 2.1 Introduction ...............................................................................................14 2.2 Safety .......................................................................................................14 2.3 Intended purpose and operating conditions.....................................................14 2.3.1 Safe conditions .....................................................................................14 2.3.2 Biohazards ...........................................................................................14 2.4 Hazardous or toxic compounds......................................................................15 2.5 Electromagnetic compatibility .......................................................................15 2.6 Emergency stop ..........................................................................................15 2.7 Power supply..............................................................................................16 2.8 Fuses ........................................................................................................17 2.9 Vacuum and pressure connection ..................................................................17 2.10 Instrument ventilation................................................................................17 2.11 Cabinet safety...........................................................................................18 2.12 384-robot pipetting head and gripper ...........................................................18 2.13 BioChip Interface (BCI) ..............................................................................18 2.14 Automated Cell Preparation module (ACP) ....................................................18 2.15 Bed of nails (BON).....................................................................................19 2.16 Disposal of consumables ............................................................................19 2.16.1 Disposal of used QChip 384 plates .........................................................19 2.16.2 Disposal of used compound plates .........................................................19 2.17 Qube embedded instrument software ...........................................................19 2.18 Moving the Qube instrument .......................................................................19 2.19 Calibrating the instrument ..........................................................................19 3. Start-up and shut-down procedures ...........................................................20 3.1 Start-up procedures and utility protocols ........................................................20 3.1.1 Qube Instrument ..................................................................................20 3.1.2 Homing the system ...............................................................................21 3.1.3 Self-test ..............................................................................................22 3.1.4 Initialize Qube – ACP and LMS ................................................................22 3.1.5 Sophion Analyzer ..................................................................................23 Qube user manual 2 Revision 2.2.10 3.2 Shut-down procedure ..................................................................................23 3.2.1 Qube Instrument ..................................................................................23 3.2.2 Database server....................................................................................24 3.2.3 Restarting Controller PC/software or Data PC/software...............................24 4. ViewPoint Maintenance ..............................................................................25 4.1 Log ...........................................................................................................25 4.2 Software ....................................................................................................25 4.2.1 How to update Qube software .................................................................26 4.2.2 How to set a fixed IP-address .................................................................27 4.3 Features ....................................................................................................28 4.4 Users ........................................................................................................28 4.5 Backup ......................................................................................................28 4.6 Protocols....................................................................................................29 4.6.1 Protocol owner and rights.......................................................................30 4.7 Protocol Runs .............................................................................................30 4.7.1 Showing log info ...................................................................................31 4.7.2 Exporting quality data............................................................................31 4.7.3 Data management ................................................................................32 4.7.4 Data reduction......................................................................................32 4.7.5 Data migration .....................................................................................34 4.7.6 Exporting protocol runs..........................................................................37 4.8 Compound lists ...........................................................................................37 4.9 Machine .....................................................................................................38