Rabbit Anti-Human LIG4 (Phospho-Thr650) polyclonal antibody (CABT-L3791) This product is for research use only and is not intended for diagnostic use. PRODUCT INFORMATION Product Overview Rabbit Anti-Human HDAC5 (Phospho-Ser498) polyclonal antibody. This antibody detects endogenous levels of DNA Ligase 4 only when phosphorylated at Thr650. Specificity Target Modification: Phospho. Modification Sites: Human: T650 Target Human DNA Ligase 4 (Phospho-Thr650) Immunogen The antiserum was produced against synthesized peptide derived from human DNA Ligase 4 around the phosphorylation site of Thr650. Immunogen range: 616-665 Isotype IgG Source/Host Rabbit Species Reactivity Human Purification Affinity Purified Conjugate Unconjugated Applications WB, IHC, ELISA Molecular Weight 103 kDa Preparation The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. Format Liquid Concentration Lot specific Buffer Rabbit IgG in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl and 50% glycerol. Preservative 0.02% Sodium Azide Storage Stable at -20°C for at least 1 year. Ship Wet ice Warnings For research use only. 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved BACKGROUND Introduction The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double- stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. Keywords LIG4;ligase IV, DNA, ATP-dependent;LIG4S;DNA ligase 4;sealase;DNA joinase;DNA ligase IV;DNA repair enzyme;polynucleotide ligase;polydeoxyribonucleotide synthase [ATP] 4; GENE INFORMATION Gene Name LIG4 ligase IV, DNA, ATP-dependent [ Homo sapiens (human) ] Official Symbol LIG4 Synonyms DNA ligase IV Entrez Gene ID 3981 UniProt ID P49917 45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected] Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved.