Anti-GNA13 Mouse mAb Catalog # PTM-5543 General Information Images Host species Mouse WB Clonality Recombinant monoclonal Blocking buffer: 5% NFDM/TBST Clone number JMMR-1693 Primary ab dilution: 1:1000 Primary ab incubation condition: 4°C Synonym / overnight Immunogen UniProt MW (kDa) Applications Secondary ab: Goat Anti-Mouse IgG H&L species (HRP) Human Q14344 44 WB, IHC-P, ICC/IF Lysate: 293, HepG2, Rat brain, N2a Protein loading quantity: 20 μg Exposure time: 30 s Product Usage Information Predicted MW: 44 kDa Application Dilution Recommended species Observed MW: 44 kDa WB 1:500-1:1000 Human IHC-P IHC-P 1:50-1:100 Human Tissue: Human testis Section type: Formalin fixed & Paraffin - ICC/IF 1:50 Human embedded section Retrieval method: High temperature and high Properties pressure Retrieval buffer: Tris/EDTA buffer, pH 9.0 Store at -20°C. Avoid freeze / thaw cycles. Storage Primary ab dilution: 1:100 Primary ab incubation condition: 1 hour at Stability Stable for 12 months from date of receipt / reconstitution room temperature Constituents PBS, Glycerol, BSA Secondary ab: Anti-Rabbit and Mouse Polymer HRP (Ready to use) Purity Protein G purified Counter stain: Hematoxylin (Blue) Isotype IgG1/Kappa Comment: Color brown is the positive signal for PTM-5543 Target Information ICC/IF Function Guanine nucleotide-binding proteins (G proteins) are Cell line: HepG2 involved as modulators or transducers in various Fixative: 100% Ice-cold methanol transmembrane signaling systems. Activates effector Permeabilization: 0.1% TritonX-100 Primary ab dilution: 1:50 molecule RhoA by binding and activating RhoGEFs Primary incubation condition: 4°C overnight (ARHGEF1/p115RhoGEF, ARHGEF11/PDZ-RhoGEF and Secondary ab: Goat Anti-Mouse IgG Nuclear counter stain: DAPI (Blue) ARHGEF12/LARG). GNA13-dependent Rho signaling Comment: Color green is the positive signal for subsequently regulates transcription factor AP-1 PTM-5543 (activating protein-1). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway. Research Use Inhibits CDH1-mediated cell adhesion in process For research use only, not for use in diagnostic procedures. independent from Rho activation. Cellular location Cytoplasm, Membrane, Nucleus APPLICATIONS WB: Western blot IP: Immunoprecipitation CONTACT US China Global IHC-P: Immunohistochemistry-Paraffin ChIP: Chromatin Immunoprecipitation Tel: 400-100-1145 +1 312-802-6843 ICC/IF: Immunocytochemistry/Immunofluorescence FC: Flow Cytometry Email: [email protected] [email protected] Web: www.ptm-biolab.com.cn www.ptmbiolabs.com .