Applied Biosystems Food Pathogen Detection System TaqMan ® Campylobacter jejuni Detection Kit Fast, easy, accurate detection TaqMan® Pathogen Detection Kits • Campylobacter jejuni • Cronobacter sakazakii • E. coli O157:H7 • Listeria monocytogenes • Pseudomonas aeruginosa • Salmonella enterica • Staphylococcus aureus A Growing Demand Real-Time PCR Delivers Faster Time to Result Campylobacter jejuni and Campylobacter The TaqMan Campylobacter jejuni Traditional Campylobacter jejuni coli cause more cases of bacteria Detection Kit uses Real-Time PCR detection methods can take up to 6 diarrheal illness than Shigella and technology designed to provide: days; immunoassay methods can Salmonella combined. However, strains take 2 days or more. After a 24 hour • Faster time to results are difficult to cultivate and low levels pre-enrichment step, time to result may go undetected using selective • Excellent specificity and sensitivity with the TaqMan Campylobacter jejuni enrichment. This has created a demand Detection Kit is less than 4 hours and • Exceptional ease of use for a rapid C. jejuni test method that less than 28 hours total. does not require isolation and culture. • Reduced risk of contamination Exceptional Ease of Use • Campylobacter jejuni 1 Results in three easy steps • Three easy steps (p/n 4366098) 1. Enrich samples • Software-guided assay procedure • Salmonella enterica 1 Certified by AOAC and AFNOR • No electrophoresis or post-PCR (p/n 4366104) processing • Cronobacter sakazakii 2 The TaqMan Campylobacter jejuni (p/n 4382492) Detection Kit gives you reliable results in just 3 simple steps. RapidFinder™ • E. coli O157:H7 1 2. Prepare samples software guides you through the assay (p/n 4366100) procedure, and displays results on an • Listeria monocytogenes 1 easy-to-read screen for fast, accurate (p/n 4366102) interpretation. • Pseudomonas aeruginosa 2 Minimal Risk of Contamination (p/n 4368604) The TaqMan assay uses a closed-tube 3. Run samples format. Once pipetted, tubes remain • Staphylococcus aureus 2 closed throughout the amplification and (p/n 4368606) detection process, minimizing risk of 1 These kits have been validated as described in cross contamination. TaqMan ® Pathogen Detection Kits–Validation Reference White Paper (stock number 116WP01-01). Preformatted and Ready to Use 2 These kits have been verified through Applied The TaqMan Campylobacter jejuni Biosystems’ internal verification procedures. Detection Kit includes reagents for These kits are based on universal assay preparation and amplification of 100 conditions, enabling you to run any Demonstrated samples, including: combination of these assays together Specificity and Sensitivity • Campylobacter jejuni target assay mix in the same sample run. The TaqMan Campylobacter jejuni Detection Kit has demonstrated: • Environmental master mix A Complete Solution • In combination with Applied Biosystems • 100% inclusivity for 27 strains Negative control Real-Time PCR Systems such as the of Campylobacter jejuni and Each reaction contains an Internal 7300, 7500, 7500Fast, and StepOne™ Campylobacter coli Positive Control (IPC) that controls for Systems, RapidFinder software, and • 100% exclusivity for 29 other the presence of inhibitors for reliable the PrepMan® Ultra Sample Preparation non-Campylobacter and non-jejuni negative results. Reagent, the TaqMan Campylobacter or coli strains Part of a Growing Family jejuni Detection Kit offers a fully- integrated, single-vendor solution that • Detects down to 1 cfu in 25 grams Applied Biosystems offers a full line of provides superior performance, reliability, or enriched food sample TaqMan Pathogen Detection Kits: and ease-of-use. For environmental (including soil, air and water), quality control, and food and finished products testing, and for research use only. Not for use in diagnostic procedures. This product has not been cleared or otherwise approved by the United States Food and Drug Administration or by any other regulatory body in any country, or under the European IVD Directive, for diagnostic or any other clinical purpose. The purchaser or user of this product agrees not to use this product for diagnostic or other clinical purposes. © Copyright 2008, 2011 Applied Biosystems. All Rights Reserved. AB (Design), Applera, Applied Biosystems and PrepMan are registered trademarks and RapidFinder and StepOne are trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 07/2011 Publication 116MI09-04 Headquarters International Sales 850 Lincoln Centre Drive | Foster City, CA 94404 USA For our office locations please call the division Phone 650.638.5800 | Toll Free 800.345.5224 headquarters or refer to our Web site at www.appliedbiosystems.com www.appliedbiosystems.com/about/offices.cfm.