PRODUCT DATA SHEET Bioworld Technology,Inc. PRPF3 polyclonal antibody Catalog: BS7788 Host: Rabbit Reactivity: Human,Mouse,Rat BackGround: IHC/IF: 1:50~1:200 PRPF3 (PRP3 pre-mRNA processing factor 3 homolog), IP 1:50 - 1:100 also known as RP18, PRP3, Prp3p, HPRP3 or HPRP3P, is Storage&Stability: an evolutionarily conserved protein involved in Store at 4°C short term. Aliquot and store at -20°C long pre-mRNA splicing and functions as a component of the term. Avoid freeze-thaw cycles. U4/U6.U5 tri-snRNP (small nuclear ribonucleoprotein) Specificity: complex. Ubiquitously expressed with predominant ex- PRPF3 polyclonal antibody detects endogenous levels of pression in retina, blood, kidney and liver, PRPF3 local- PRPF3 protein. izes to nuclear speckles and is phosphorylated in vitro. DATA: PRPF3 directly interacts with PRPF4 and is present in the inactive spliceosome but is not found in the catalytically active spliceosome. Mutations in the gene encoding PRPF3 result in autosomal dominant retinitis pigmentosa type 18 (RP18), which leads to photoreceptor cell degen- eration. RP18 patients initially exhibit a loss of their midperipheral visual field as well as night vision blind- ness. The disease eventually progresses to the loss of far WesternBlot (WB) analysis of PRPF3 polyclonal antibody peripheral visual field and finally the loss of central vi- sion. This suggests that PRPF3 is a key player in the pre-mRNA splicing of photoreceptor-specific genes. Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Molecular Weight: ~ 100 kDa Immunofluorescence analysis of U2OS cells, using PRPF3 polyclonal Swiss-Prot: antibody O43395 Note: Purification&Purity: For research use only, not for use in diagnostic procedure. The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific im- munogen and the purity is > 95% (by SDS-PAGE). Applications: WB: 1:500~1:2000 Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .