TEF Antibody Cat. No.: 28-837 TEF Antibody Antibody used in IHC on Human Skeletal muscle at 5 ug/ml. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human TEF. TESTED APPLICATIONS: ELISA, WB TEF antibody can be used for detection of TEF by ELISA at 1:312500. TEF antibody can be APPLICATIONS: used for detection of TEF by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1211 - HepG2 Cell Lysate PREDICTED MOLECULAR 33 kDa WEIGHT: September 29, 2021 1 https://www.prosci-inc.com/tef-antibody-28-837.html Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store TEF STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: TEF ALTERNATE NAMES: TEF, KIAA1655 ACCESSION NO.: NP_003207 PROTEIN GI NO.: 4507431 GENE ID: 7008 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References TEF (thyrotroph embryonic factor) is a member of the PAR bZip (proline and acidic amino acid-rich basic leucine zipper) transcription factor family. It accumulates with robust circadian rhythms in tissues with high amplitudes of clock gene expression.Thyrotroph embryonic factor (TEF), a transcription factor, is a member of the PAR (proline and acidic amino acid-rich) subfamily of basic region/leucine zipper (bZIP) transcription factors. It is expressed in a broad range of cells and tissues in adult animals, however, during embryonic development, TEF expression appears to be restricted to the developing BACKGROUND: anterior pituitary gland, coincident with the appearance of thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to, and transactivate the TSHB promoter. It shows homology (in the functional domains) with other members of the PAR-bZIP subfamily of transcription factors, which include albumin D box-binding protein (DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin gene-binding protein (VBP); VBP is considered the chicken homologue of TEF. Different members of the subfamily can readily form heterodimers, and share DNA-binding, and transcriptional regulatory properties. REFERENCES: 1) Inukai, T., (2005) Blood 105 (11), 4437-4444. ANTIBODIES FOR RESEARCH USE ONLY. September 29, 2021 2 https://www.prosci-inc.com/tef-antibody-28-837.html For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 3 https://www.prosci-inc.com/tef-antibody-28-837.html.