Small Animal Physiological Monitoring System ECG • Respiration • Heart Rate • Warming & Temperature Blood Pressure • SpO2 • EtCO2 Small Footprint Integrated & Ergonomic Intuitive Touchscreen Real-Time Numeric & Graphic Display Small Animal Physiological Monitoring System System shown with mouse platform The Small Animal Physiological Monitoring System is an instrument integrating multiple physiological parameters on one single small platform. The objective behind this new instrument is to provide The Physiological Monitoring System is available superior monitoring results, while making surgery and with a stereotaxic adaptor option, which includes ear bars, other manipulations on small animals easier. a tooth bar and either a nose bar or an anesthesia mask. The stereotaxic device is fixed to the platform, but can The platform integrates monitoring of the rectal easily be removed depending upon the application. temperature, electrocardiogram (ECG), respiration, oxygen saturation (SpO2), blood pressure and exhaled The Physiological Monitoring System comes complete CO2 (EtCO2). It also includes a controlled heating with a heated platform (mouse or rat and mouse), surface to maintain the animal’s body temperature at an Android tablet with case and stand, Bluetooth the desired level. communication module, a rectal probe and electrode gel. The system has many benefits regarding surgical Data can be saved and comments can be added with procedures. It demands less installation time at the the press of a button. At the end of experiments, recorded beginning of procedures and it reduces the required data can easily be transferred to any computer for space and the number of wires around the animal. analysis. Scripts and utilities are provided to convert A single data/power cable connects to a small wireless data in LabChart or CSV format and to display signals in communication module. All data is transferred to an Excel and Matlab. Android tablet by Bluetooth for display and saving. The Physiological Monitoring System features a real- time display which provides numeric values as well as waveforms that can be customized by the user. Multiple signals can be displayed on each of the three graphs. Harvard Apparatus Physiological Monitoring System Heated Platform Single Data/Power Cable Link to a compact Bluetooth module with 4 analog outputs Exhaled CO Ports Warming Zone 2 0.1°C resolution Respiration Sensor ECG Electrodes External Ports Rat and mouse • Pressure transducer platform shown • Pulse ox sensor • Rectal temperature probe • ECG electrodes Mouse platform with Stereotaxic Adaptor Physiological Advanced Parameters Display Interface ECG Integrated electrodes under Touchscreen display the paws & external electrodes Pinch to zoom on waveforms (works with surgical gloves). Respiration Waveform acquired with a sensor under the animal 1 to 5 second waveforms Select any signal to display. Heart & Breath Rate Real-time display from the ECG and respiration waveform 1 to 30 minute trends View temperature, heart & breath Warming & Temperature rate trends, and numeric values. Heated platform and rectal probe for precise control and monitoring Easy data saving Blood Pressure (option) Add notes, save and export Connection for a pressure transducer data for an easy analysis. (purchased separately) SpO2 (option) Pulse oximetry and oxygen saturation with a paw/tail sensor (included with option) Exhaled CO2 (option) Gas ports for precise CO2 measurements (must be used with an external ventilator) www.harvardapparatus.com • [email protected] • 800.547.6766 Example Waveforms ECG and respiration signals of a mouse Zoomed ECG Lead 1 of a mouse 0 1 0.5 0.8 0.6 0 50 0.4 -0.5 0.2 -1 25 0 -0.2 ECG Lead I (mV) ECG Lead I (mV) -0.4 0 Respiration signal (A.U.) -0.6 -0.8 -25 0 0.5 1 1.5 2 2.5 3 0 0.05 0.1 1.15 0.2 0.25 0.3 Time in seconds Time in seconds Heart and respiration rates of a mouse over 2 hours Rectal temperature (ºC) of a mouse over 3 hours 550 250 37.25 37.2 37.15 500 200 37.1 37.05 450 150 37 36.95 Heart rate (BPM) 36.9 Respiration rate (BPM) Rectal temperature (ºC) Rectal temperature 400 100 36.85 36.8 50 36.75 350 0 20 40 60 80 100 120 0 20 40 60 80 100 120 140 160 180 Time in seconds Time in seconds Left ventricular pressure of a mouse Exhaled CO2 and heart rate of a dying mouse 1.5 700 80 70 1.25 600 60 50 1 (%) 2 500 40 0.75 30 400 20 Exhaled CO 0.5 Heart rate (BPM) 10 Blood pressure (mmHg) Blood pressure 300 0 0.25 -10 0 200 -20 0 5 10 15 20 25 30 35 40 0 0.5 1 1.5 2 2.5. 3 Time in seconds Time in seconds Harvard Apparatus Physiological Monitoring System Detailed Specifications System Information ECG Heated Platform • Display Leads I, II, III, aVL, aVR & aVF • Easy to clean surface • Supine or prone position • 10 x 21 x 2.5 cm (mouse) • 1 kHz, 24-bit acquisition • 12 x 30 x 2.5 cm (rat and mouse) • 3 external electrode connections (2 mm) Tablet Respiration • 8-inch in Android tablet (10.1-inch available) • 250 Hz acquisition • High resolution touchscreen display • Heart and breath rate • Capacity of over 400 hours of saved data • 200 to 800 beats/min • 5-8 hours battery life • 25 to 330 breaths/min • USB cable and charger • Calculated every second Communication Module Warming & Temperature • Bluetooth and analog outputs are in a separate enclosure • Heated surface up to 45°C to avoid interference • 0.1°C resolution • 9 x 11 x 2.5 cm • Closed loop PID controller keeps the animal • 100-240 V power supply within ±0.1°C of set temperature • Bluetooth • 15 to 25 meters typical range between the communication Blood Pressure module and the display unit • Full waveform display • Systolic and diastolic numeric values Analog Outputs • Comes standard with Redel 5-pin connector • 4 configurable outputs • Adaptor available for 8-pin DIN connector • BNC connectors, ±5 V range • 250 Hz acquisition, 24-bit bridge amplifier • 1 kHz, 16-bit refresh rate Data Analysis Exhaled CO2 • Connect ventilator directly to the platform (1/8 inch tubing) • .csv conversion tool • 1 Hz refresh rate • MATLAB® & Excel® import and display scripts • 0.1% accuracy • Compatible with third-party analysis software (LabChart) SpO2 • 80 to 100% saturation For more complete product details, and part • 250 Hz, 24-bit acquisition number listings, please visit our website: • Red and infrared channel display www.harvardapparatus.com Ordering Information Order No. Product 73-4905 APT300 Pressure Transducer for Order No. Product HA Physiological Monitoring System 75-1500* Physiological Monitoring System for Mouse 77-0207 ECG 3-Needle Electrodes, 2 mm pin (10g to 100g) 75-1540 Stereotaxic Kit with Gas Anesthesia Mask for 75-1501* Physiological Monitoring System for Rat 75-1500 Platform and Mouse (10g to 600g) 75-1541 Stereotaxic Kit with Nose Clamp for 75-1500 75-1502 Blood Pressure Option Platform (BP Transducer purchased separately) 75-1542 Stereotaxic Kit with Gas Anesthesia Mask for 75-1503 End Tidal CO2 Option 75-1501 Platform (for mouse only) 75-1504 SpO2 Option 75-1543 Stereotaxic Kit with Nose Clamp for (Pulse Ox Sensor included with option) 75-1501 Platform (for mouse only) *NOTE: At the end of the part number add EU for Europe, UK for United Kingdom and CN for China models. Harvard Apparatus Physiological Monitoring System Contact us for more information! CMA Microdialysis, AB Torshamnsgatan 30A SE-164 07 Kista, Sweden Harvard Apparatus China Hugo Sachs Elektronik / Harvard Apparatus, GmbH Room 1902E, 19F, Building B phone +46.8.470.10.00 Gruenstrasse 1 Zhong Shan Plaza March-Hugstetten D-79232, Germany e-mail [email protected] 1065 West Zhong Shan Road web www.microdialysis.se Changning District phone +49.0.7665.9200.0 Shanghai, China fax +49.0.7665.9200.90 phone +86.21.2230.5128 e-mail [email protected] e-mail [email protected] web www.hugo-sachs.de Harvard Apparatus 84 October Hill Road Holliston, MA 01746, USA U.K. Panlab, S.L. / Harvard Apparatus Spain phone +1.508.893.8999 C/Energia, 112 08940 Cornellà Biochrom Limited - Harvard Apparatus UK toll free +1.800.272.2775 (USA Only) Barcelona, Spain fax +1.508.429.5732 East Wing, Building 1020 Cambourne Business Park, Cambourne phone +34.934.750.697 (International Sales) e-mail [email protected] Cambridge, CB23 6DW, United Kingdom + 34.934.190.709 (Sales in Spain) web www.harvardapparatus.com fax +34.934.750.699 phone +44.1732.864001 e-mail [email protected] fax +44.1732.863356 web www.panlab.com e-mail [email protected] web www.harvardapparatus.co.uk Harvard Apparatus Canada 6010 Vanden Abeele Saint-Laurent, Quebec France H4S 1R9, Canada phone +1.514.335.0792 Harvard Apparatus, S.A.R.L. toll free +1.800.361.1905 (CAN Only) 6 Avenue des Andes fax +1.514.335.3482 Miniparc Building 8 e-mail [email protected] 91952 Les Ulis Cedex, France web www.harvardapparatus.ca phone +33.1.64.46.00.85 fax +33.1.64.46.94.38 e-mail [email protected] web www.harvardapparatus.fr divisions of Harvard Bioscience, Inc..