Revista de Ciências Farmacêuticas Básica e Aplicada Rev. Ciênc. Farm. Básica Apl., v. 26, n.2, p. 145-148, 2005 Journal of Basic and Applied Pharmaceutical Sciences ISSN 1808-4532 Analysis of susceptibility profile of Pseudomonas spp. and prevalence of bacterial samples from the surfaces of dental consulting-rooms Pietro, R.C.L.R.1,2*; Kashima, S.1; Almeida, A.M.F.1; Silva, C.H.P.M.3; Rocha, L.B.4; Pádua, J.M.4; Lia, R.C.C.4 1Curso de Ciências Farmacêuticas da Universidade de Ribeirão Preto,UNAERP; 2Departamento de Fármacos e Medicamentos da 3 4 Faculdade de Ciências Farmacêuticas de Araraquara, UNESP; Curso de Odontologia, FAESA; Curso de Odontologia da Universidade de Ribeirão Preto, UNAERP Recebido 15/09/05 / Aceito 21/11/05 ABSTRACT presence in the environment. The objective of this study was to analyze the The aim of this research was to evaluate the susceptibility susceptibility profile of P.aeruginosa, one of the most profile of Pseudomonas spp. and the prevalence of prevalent bacteria involved in cross-contamination, and the bacterial samples isolated from horizontal surfaces incidence of microbial contamination on surfaces in dental surrounding wash-basins used by dentists in several consulting-rooms, near to and distant from the dentists’ adjoined consulting-rooms, at points next to and at a wash basins, with the purpose of revealing any high risks distance from the basin, before and after surgical of infections that could be reduced with effective procedures. Our results showed a high percentage of contamination control procedures. Gram-positive cocci and Gram-negative bacilli; 34.66% were Staphylococcus spp. and 30.12% were non- MATERIAL AND METHODS fermentative Gram-negative bacilli among which Pseudomonas spp. (40.90%) was the commonest genus. Sample Collection: Samples (1246 isolates) were taken Analysis of the susceptibility profile of Pseudomonas spp. from the surfaces of several adjoining dental consulting- isolates by determining the minimal inhibitory rooms with cotton swabs. The samples were collected once concentration (MIC) of 14 antibiotics showed a great before and once after clinical procedures, near to and distant variation among the strains and high rates of resistance from the wash basins used by the dentists. to cefazolin, ceftazidime and aztreonan. Of the 14 Isolation and identification of microorganisms: By using antibiotics tested, 59.03% were found to be active against conventional procedures to collect the samples, each sample, all the environmental isolates. Strains were resistant to after bacterial enrichment in BHI broth (Brain Heart aztreonan (62.82%), while susceptibility to third Infusion broth, Merck, Darmstadt, Germany), was streaked generation cephalosporins was variable. on 5% sheep blood agar, MacConkey agar and mannitol Keywords: Pseudomonas, susceptibility, dental consulting- salt agar to allow differentiation of microorganisms. room, P. aeruginosa, P. stutzeri. Bacterial strains were isolated and identified in accordance with the Manual of Basic Procedures in Medical INTRODUCTION Microbiology for the Control of Nosocomial Infection (Brasil, 1991), by streaking one loopful on selective and Pseudomonas aeruginosa is an opportunistic nonselective media. Microorganisms of medical importance pathogen that causes urinary tract infections, respiratory were identified to genus, species and subspecies levels. system infections, dermatitis, soft tissue infections, Commercial kits (Probac do Brasil Ltda., São Paulo, SP, bacteremia and a great variety of systemic infections (Bodey Brazil), Gram-negative Combo 20 panel (MicroScan et al., 1983; Agarwal et al., 2005). The typical Pseudomonas System, Dade-Behring, USA) and/or traditional methods bacterium in nature might be found in a biofilm, and is one were used to identify bacterial samples (Balows et al., 1991). of the most vigorous, fast-swimming bacteria seen in Testing was performed according to manufacturer’s infusions and water samples (Hardalo & Edberg, 1997; instructions. Costerton et al., 1999). This species is found all over the Susceptibility Tests: The antimicrobial susceptibility world, and may be present as part of the normal flora of profiles were determined by microdilution broth method. humans, although the prevalence of colonization of healthy The Minimal Inhibitory Concentration (MIC) was individuals outside the hospital is rather low (Moss, 1995). determined using Negative MIC Plus Panel Type 3 While colonization usually precedes infections by P. (MicroScan system, Dade-Behring, USA) and the drugs/ aeruginosa, the exact source and mode of transmission of MIC range (in µg/mL) tested were cefazolin/2-32 (CZ), the pathogen are often unclear because of its ubiquitous ceftazidime/1-32 (CAZ), cefotaxime/2-64 (CFT), *Corresponding author: Rosemeire C. L. R. Pietro - Curso de Ciências Far- macêuticas da Universidade de Ribeirão Preto-UNAERP. Avenida Costábile Romano, 2201, 14096-380, Ribeirão Preto, São Paulo, Brazil. E-mail: [email protected], Fone: +55-16-6036748, Fax: +55-16-6036705. 145 Susceptibility profile of Pseudomonas ceftriaxone/2-64 (CAX), cefepime/2-16 (CPE), piperacillin/ general analysis of susceptibility profiles, carried out as 8-64 plus tazobactam/4 (PTZ), netilmicin/2-16 (NT), recommended in M7-A5/M100-S10 (NCCLS, 2000) meropenem/1-8 (MER), imipenem/0.5-16 (IMP), azlocillin/ showed that 18.77% of strains were resistant to all tested 64 (AZ), aztreonam/1-32 (AZT), ciprofloxacin/0.25-4 (CIP), drugs, and of 156 tests performed, 23.53% of antimicrobial ticarcillin/16-128 plus clavulanic acid/2 (TIM) and agents were ineffective. To the monobactam, aztreonam, mezlocillin/16-128 (MZ). The results were classified into 62.82% of environmental isolates showed resistance and to susceptible, intermediate or resistant, as per CLSI (Clinical cefotaxime and cefazolin the percentages were 39.10 and and Laboratory Standard Institute) guidelines (Balows et 100% of resistance, respectively. The drugs with high al. 1991; NCCLS, 2000). activity against all the environmental isolates of Pseudomonas spp. were meropenem, imipenem, RESULTS ciprofloxacin, ticarcillin and mezlocillin. (Table 2). In this study, 1246 isolates were taken from the surfaces around wash-basins, close to and distant from the Table 1 - Identification of Pseudomonas spp. isolated from basins, in dental surgeries, before and after surgical the surfaces of wash basin around sink (PP), wash procedures. The most prevalent group of bacteria found were basin distant sink (PD) of dental offices before the staphylococci (34.66%), followed by non-fermentative (A) and after dental surgical procedures (B). Gram-negative bacilli, contributing 30.12%. Samples/Isolates Microorganisms The incidence of Pseudomonas spp. was 40.09% of PPA/20 Pseudomonas aeruginosa the total Gram-negative bacilli, 145 isolates (92.94%) being collected around the basins and 7.05% (11 isolates) distant PPA/7 Pseudomonas aeruginosa from them. (Table 1). PPA/32 Pseudomonas aeruginosa Given the pathogenic potential of this non- PPA/33 Pseudomonas aeruginosa fermentative Gram-negative bacillus, some Pseudomonas PPA/23 Pseudomonas stutzeri strains were tested for in vitro susceptibility. The samples PPB/7 Pseudomonas stutzeri tested were selected on the basis of their highly variable PPB/22 Pseudomonas aeruginosa susceptibility. Fifty percent of all antibiotics tested inhibited PPB/1 Pseudomonas aeruginosa all Pseudomonas spp. strains. Third and fourth generation cephalosporins possessed lower activity against the isolates, PDA/5 Pseudomonas aeruginosa which showed between 17.30 and 79.48% of resistance. The PDA/6 Pseudomonas stutzeri Table 2 - MIC values (µg/mL) of the 156 samples of Pseudomonas spp. isolated from the surfaces of wash basin around the sink (PP), wash basin distant from sink (PD) of dental offices before (A) and after dental surgical procedures (B) of dental offices. Antibioticsa Samples PPA PPA PPA PPA PPA PDA PDA PPB PPB PPB 20 7 32 33 23 5 6 7 22 1 CZ > 32 (R) b > 32 (R) > 32 (R) > 32 (R) > 32 (R) > 32 (R) > 32 (R) > 32 (R) > 32 (R) > 32 (R) CAZ > 32 (R) 16 (I) c 8 (S) d 8 (S) 8 (S) 2 (S) 8 (S) 4 (S) 8 (S) 8 (S) CFT > 64 (R) > 64 (R) 32 (I) 8 (S) > 64 (R) > 64 (R) > 64 (R) 32 (I) 32 (I) 32 (I) CAX > 64 (R) 32 (I) 16 (S) 8 (S) > 64 (R) 32 (I) 8 (S) 8 (S) 8 (S) 16 (S) CPE 2 (S) < 2 (S) > 16 (R) 2 (S) 2 (S) < 2 (S) < 2 (S) 2 (S) < 2 (S) < 2 (S) PTZ < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) < 8 (S) NT 8 (S) 4 (S) 8 (S) 4 (S) 16 (I) 4 (S) 4 (S) 8 (S) 4 (S) 4 (S) MER < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) < 1 (S) IMP < 0.5 (S) < 0.5 (S) 1 (S) 1 (S) < 0.5 (S) < 0.5 (S) < 0.5 (S) 1 (S) < 0.5 (S) < 0.5 (S) AZ < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) < 64 (S) AZT > 32 (R) 4 (S) > 32 (R) 4 (S) > 32 (R) 4 (S) 4 (S) 4 (S) > 32 (R) > 32 (R) CIP 1 (S) 1 (S) 1 (S) 1 (S) 1 (S) < 0.25 (S) < 0.25 (S) 1 (S) 1 (S) <0.25 (S) TIM < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) MZ < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) < 16 (S) a Antibiotics: cefazolin (CZ), ceftazidime (CAZ), cefotaxime (CFT), ceftriaxone (CAX), cefepime (CPE), piperacillin plus tazobactam (PTZ), netilmicin (NT), meropenem (MER), imipenem (IMP), azlocillin (AZ), aztreonam (AZT), ciprofloxacin (CIP), ticarcillin plus clavulanic acid (TIM) and mezlocillin (MZ). b (R): resistant; c (I): intermediate; d (S): sensible. 146 Susceptibility profile of Pseudomonas DISCUSSION that part of isolates of the Pseudomonas spp contamination could occur during the surgical procedures, when aerosols The transmission of infection during dental would be disseminated to places nearby, carrying mainly procedures can occur by direct contact with tissues and the resistant strains.