Catalog # Aliquot Size M41-911-05 3 x 5 nmol M41-911-20 3 x 20 nmol M41-911-50 3 x 50 nmol MAPKAPK3 siRNA Set I siRNA duplexes targeted against three exon regions Catalog # M41-911 Lot # Z2049-79 Specificity Formulation MAPKAPK3 siRNAs are designed to specifically knock- The siRNAs are supplied as a lyophilized powder and down human MAPKAPK3 expression. shipped at room temperature. Product Description Reconstitution Protocol MAPKAPK3 siRNA is a pool of three individual synthetic Briefly centrifuge the tubes (maximum RCF 4,000g) to siRNA duplexes designed to knock-down human collect lyophilized siRNA at the bottom of the tube. MAPKAPK3 mRNA expression. Each siRNA is 19-25 bases in Resuspend the siRNA in 50 µl of DEPC-treated water length. The gene accession number is NM_004635. (supplied by researcher), which results in a 1x stock solution (10 µM). Gently pipet the solution 3-5 times to mix Gene Aliases and avoid the introduction of bubbles. Optional: aliquot 3PK; 3pK; MAPKAP3 1x stock solutions for storage. Storage and Stability Related Products The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the Product Name Catalog Number lyophilized and resuspended siRNAs are stored at or MAPKAPK2, Active M40-11G below -20oC. After resuspension, siRNA stock solutions ≥2 MAPKAPK2, Active M40-11H µM can undergo up to 50 freeze-thaw cycles without MAPKAPK3, Active M41-10G significant degradation. For long-term storage, it is MAPKAPK5, Active M42-10G recommended that the siRNA is stored at -70oC. For most Anti-MAPKAPK2/3 M40-63R favorable performance, avoid repeated handling and MAPKAPK2, Unactive M40-14G multiple freeze/thaw cycles. MAPKAPK3, Unactive M41-14G Scientific Background MAPKAPK3 is closely related to MAPKAPK2 sharing 72% nucleotide and 75% amino acid identity (1). MAPKAPK3 is MAPKAPK3 siRNA Set I activated by growth inducers and stress stimulation of siRNA duplexes targeted against three exon regions cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to Catalog Number M41-911 phosphorylate and activate this kinase, which suggested Specific Lot Number Z2049-79 the role of MAPKAPK3 as an integrative element of Packaging Specifications 2.5 nmol/tube for 3 x 5 nmol signaling in both mitogen and stress responses (2). Format Lyophilized powder Stability 1yr at -70oC from date of shipment Storage & Shipping The lyophilized powder is stable for at References least 4 weeks at room temperature. It is 1. Sithanandam, G. et al: 3pK a new mitogen-activated recommended that the lyophilized protein kinase-activated protein kinase located in the and resuspended siRNAs are stored at o small cell lung cancer tumor suppressor gene region. or below -20 C. After resuspension, siRNA stock solutions ≥2 µM can Molec. Cell. Biol. 16: 868-876, 1996. undergo up to 50 freeze-thaw cycles 2. Ludwig, S. et al: 3pK, a novel mitogen-activated without significant degradation. For protein (MAP) kinase-activated protein kinase, is long-term storage, it is recommended that the siRNA is stored at -70oC. For targeted by three MAP kinase pathways. Molec. Cell. most favorable performance, avoid Biol. 16: 6687-6697, 1996. repeated handling and multiple freeze/thaw cycles. To place your order, please contact us by phone 1-(604)-232-4600, fax 1-604-232-4601 or by email: [email protected] www.signalchem.com FOR IN VITRO RESEARCH PURPOSES ONLY. NOT INTENDED FOR USE IN HUMAN OR ANIMALS. .