MAP2K2 Antibody Cat. No.: 63-309 MAP2K2 Antibody Fluorescent image of U251 cell stained with MAP2K2 Antibody (T394). U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then Formalin-fixed and paraffin-embedded human prostata incubated with MAP2K2 primary antibody (1:25). For carcinoma tissue reacted with MAP2K2 Antibody (T394) , secondary antibody, Alexa Fluor 488 conjugated donkey which was peroxidase-conjugated to the secondary anti-rabbit antibody (green) was used (1:400).Cytoplasmic antibody, followed by DAB staining. actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). MAP2K2 immunoreactivity is localized to Cytoplasm significantly. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated IMMUNOGEN: synthetic peptide between 372-400 amino acids from human MAP2K2. TESTED APPLICATIONS: IF, IHC-P, WB September 26, 2021 1 https://www.prosci-inc.com/map2k2-antibody-63-309.html For WB starting dilution is: 1:1000 APPLICATIONS: For IF starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:10~50 PREDICTED MOLECULAR 44 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: MAP2K2 Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ALTERNATE NAMES: ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2 ACCESSION NO.: P36507 PROTEIN GI NO.: 547915 GENE ID: 5605 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this BACKGROUND: kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax. September 26, 2021 2 https://www.prosci-inc.com/map2k2-antibody-63-309.html REFERENCES: 1) Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003). 2) Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003). 3) Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003). 4) Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003). ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/map2k2-antibody-63-309.html.