Free-living and Symbiotic Plathelminthes Proceedings of the Fifth International Symposium on the Biology of «Turbellarians» held at Gottingen, Federal Republic of Germany, August 9 — 14, 1987 Edited by Peter Ax, Ulrich Ehlers, Beate Sopott-Ehlers With the assistance of the Editorial Board Ian R. Ball, Patrick J. S. Boaden, Michael D. B. Burt 381 figures and 22 tables .SEMPER \ k-*B0NlS AKTIBUS' Gustav Fischer Verlag • Stuttgart • New York -1988 Contents Symbiosis and Parasitism Jennings, J. B.: Nutrition and respiration in symbiotic Turbellaria................................................... 1 Burt, M. D. B.: Recent advances in our knowledge of parasitic Turbellaria....................................... 15 Christensen, A. M.: Fecampiidae (Turbellaria, Neorhabdocoela) in Greenland waters .......................... 25 MacKinnon, B. M.: Preliminary investigation of the reproductive biology of Pseudostomum sp., a com­ mensal prolecithophoran on Echinorachnius parma, with emphasis on the ultra­ structure of the embryo and eggshell......................................................................... 31 Jondelius, U.: Epidermal ultrastructure of adults and juveniles of Triloborhynchus astropectinis (Dalyellioida, Platyhelminthes)................................................................................... 39 Kornakova, E. E.: On morphology and phylogeny of Udonellida......................................................... 45 Xylander, W. E. R.: Ultrastructural studies on Udonellidae: evidence for a position within the Neoder- mata............................................................................................................................ 51 Joffe, B. I.: On the phylogeny of the Temnocephalida................................................................ 59 Regeneration and Differentiation Baguna, J., E. Salo, J. Collet, C. Auladell and M. Ribas: Cellular, molecular and genetic approaches to regeneration and pattern formation in planarians................................................................................................................ 65 Ghirardelli, E., R. Marzari, E. A. Ferrero and M. Bittolo: Determination and differentiation in planarian regeneration: molecular aspects of blastema heterogeneity................................................................................................ 79 Hori, I.: Unusual nuclei of regenerating cells in the early blastema of the planarian Dugesia japonica.....................................■.............................................................................. 85 Teshirogi, W. and K. Tohya: Primary tissue culture of freshwater planarians in a newly devised medium............. 91 Drobysheva, I. M.: An autoradiographic study of the replacement of epidermis in polyclad turbella- rians............................................................................................................................. 97 XIV • Contents Pascolini, R., S. Lorvik and M. Camatini: Migrating cells during wound healing of Dugesia lugubris s.l..................................... 103 Schurmann, W. and R. Peter: Isolation and cultivation of planarian neoblasts by a novel combination of methods 111 Karyology and Cytology Kawakatsu, M., I. Oki, S. Tamura and K. Sekiguchi: Karyological and taxonomic studies of ectoparasitic marine triclads collected from the four extant species of horseshoe crabs................................................................ 117 Tamura, S., I. Oki and M. Kawakatsu: Karyological and taxonomic studies of Dugesia japonica from the Southwest Is­ lands of Japan ................................................ 123 Ribas, M., M. Pala, R. A. Vacca, M. Riutort and J. Baguna: Taxonomic status of the western Mediterranean asexual populations of the Dugesia (D) gonocephala group. Morphological, karyological and biochemical da­ ta ................................................................................................................................ 129 Oki, I., S. Tamura, R. E. Ogren and M. Kawakatsu: Karyological and taxonomic studies of three species of the genus Bipalium from Japan and the United States and Platydemus manokwari from the Philippines . 139 Ponce de Leon, R.: Karyotypes of two species of Temnocephala (Platyhelminthes)................................ 145 Morita, M., F. L. Hall and J. B. Best: Nucleocytoplasmic interaction on the de novo biogenesis of planarian mitochon­ dria ............................................................................................................................. 151 Lindroos, P. and M. J. Still: Extracellular matrix components in Polycelis nigra (Turbellaria, Tricladida) .... 157 Hori, H., A. Muto, S. Osawa, M. Takai, K.-Y. Lue and M. Kawakatsu: Evolution of Turbellaria as deduced from 5S ribosomal RNA sequences................ 163 Peter, R.: A preliminary report on the adenylate system in the planarian Crenobia alpina . 169 Ratz, H., A. Faubel and T. Roder: Electrophoresis and histochemistry as tools in the taxonomy of the Acoela............. 173 Kerschbaum H. H., K. Treiblmayr and K. Pohlhammer: Localization of 5-Ht and gastrin-cck-immunoreactivity in Crenobia alpina (Tri­ cladida, Plathelminthes)............................................................................................. 177 Nervous System and Sensory Structures Reuter, M.: Development and organization of nervous systems visualized by immunocy­ tochemistry in three flatworm species ...................................................................... 181 Contents • XV Golubev, A. I.: Glia and neuroglia relationships in the central nervous system of the Turbellaria (Electron microscopic data) ...................... 185 Kotikova, E. A. and B. I. Joffe: On the nervous system of the dalyellioid turbellarians............................................. 191 Trawicki, W., A. Czubaj and J. Moraczewski: The brain ultrastructure of Dendrocoelum lacteum (O. F. Muller).......................... 195 Koopowitz, H. and C. Huynh: Observations on the visual system in Notoplana acticola, a polyclad flatworm ... 201 Morita, M., F. L. Hall and J. B. Best: An optic neurosecretory cell in the planarian............................................................. 207 Palmberg, I., G. Dahlvik, V. I. Govardovskii, M. Lindstrom, M. Reuter and T. Reuter: Are the eyespots of Microstomumlineare light-sensitive?.......................................... 211 , Sopott-Ehlers, B.: Fine structure of photoreceptors in two species of the Prolecithophora................... 221 Tyler, S. and M. D. B. Burt: Lensing by a mitochondrial derivative in the eye of Urastoma cyprinae (Turbellaria, Prolecithophora).......................................................................................................... 229 Lanfranghi, A. and C. Bedini: The ultrastructure of the eyes of Rhynchomesostoma rostratum (Muller, 1774) (Turbellaria, Rhabdocoela) ...................................................................................... 235 Reproduction and Ontogeny Gremigni, V.: A comparative ultrastructural study of homocellular and heterocellular female gonads in free-living Platyhelminthes-Turbellaria ................................................... 245 Smith, J. P. S., M. B. Thomas, R. Chandler and S. F. Zane: Granular inclusions in the oocytes of Convoluta sp., Nemertoderma sp., and Nemertinoides elongatus (Turbellaria, Acoelomorpha)............................................. 263 Sakurai, T.: Electron microscopic studies of the oogenesis in the freshwater tri clad, Bdel- locephala brunnea...................................................................................................... 271 Boyer, B. C.: The effect of removing vegetal cytoplasm during the maturation divisions on the development of Hoploplana inquilina (Turbellaria, Polycladida)............................. 277 Romero, R. and J. Baguna: Quantitative cellular analysis of life-cycle strategies' of iteroparous and semelparous triclads............................................................................................................... 283 Ishii, S. and T. Sakurai: Scanning and transmission electron microscopic observations on epidermal de­ velopment in the embryo of the freshwater triclad, Bdellocephala brunnea............. 291 XVI • Contents Ishida, S. and W. Teshirogi: Comparison of spermatozoa among freshwater planarian species .......................... 297 L’Hardy, J.-P.: Sperm morphology in Kalyptorhynchia (Platyhelminthes, Rhabdocoela)................ 303 Cifrian, B., S. Martinez-Alos and P. Garcia-Corrales: Ultrastructural study of spermatogenesis and mature spermatozoon of Both- romesostoma personatum (Rhabdocoela, Typhloplanoida)....................................... 309 Bruggemann, J.: Ultrastructure and differentiation of the penis stylet of Pogaina kinnei Ax, 1970 (Plathelminthes, Rhabdocoela)................................................................................... 315 Hendelberg, J. and B. Akesson: Convolutriloba retrogemma gen. et sp. n., a turbellarian (Acoela, Platyhelminthes) with reversed polarity of reproductive buds ............................................................. 321 Morphology and Phylogeny Tyler, S.: The role of function in determination of homology and convergence - examples from invertebrate adhesive organs............................................................................. 331 Ivanov, A. V.: On the early evolution of the Bilateria...................................................................... 349