TM Vectra® Polaris Automated Quantitative Pathology Imaging System User Manual Vectra Polaris 1.0.11 Publication Date: October 24, 2019 Notice This manual is published by Akoya Biosciences, Inc. The information in this document is subject to change without notice and should not be construed as a commitment by Akoya Biosciences, Inc. Akoya assumes no responsibility for any errors that may appear in this document. This manual is believed to be complete and accurate at the time of publication. In no event shall Akoya Biosciences be liable for incidental or consequential damages in connection with or arising from the use of this manual. This manual describes how to use Vectra Polaris software. For more information contact: Akoya Biosciences, Inc. 100 Campus Drive, 6th Floor, Marlborough, MA, 01752, USA Phone: +1 855-896-8401 Fax: +1 855-404-0061 Email: [email protected] Email (outside the US): [email protected] Web site: http://www.akoyabio.com This software covered by US Patent 7,555,155; 7,655,898; and patents pending. Table of Contents Chapter 1 Preface..................................................................................................... 5 Chapter 2 Instrum..e..n..t. .S..a..f.e..t.y..................................................................................... 11 1 Re.q..u..ir.e..d.. .T..r.a.i.n..i.n.g.................................................................................................................... 11 2 Ele..c..t.r.ic..a..l. .S..a..fe..t.y..................................................................................................................... 11 Pow.e.r. .C...o.r.d.. .S..e..l.e..c.t.i.o..n.. ..................................................................................................... 12 Fuse..s. ............................................................................................................................ 12 Cabl.e..s. .a..n..d.. .A..d..a.p..t.e..r.s.. ...................................................................................................... 13 3 Me..c.h..a..n..ic..a..l. .S..a..fe..t.y.................................................................................................................. 13 4 We..i.g..h.t. .W....a.r.n..i.n..g..................................................................................................................... 13 5 Bri.g..h..t. .L.i.g..h..t........................................................................................................................... 13 Chapter 3 Introdu.c..t.i.o..n.............................................................................................. 15 1 Int.e.n..d..e..d. .U...s.e.......................................................................................................................... 15 2 Pri.n..c..ip..l.e..s. .o..f. .O..p..e..r.a..t.io..n............................................................................................................ 15 Exam...p..le.. .A..p..p..l.ic..a..t.i.o.n..s.. ..................................................................................................... 17 3 Th.e.o..r.y.. .o..f .I.m...a..g.i.n..g................................................................................................................... 17 Light. .............................................................................................................................. 18 Hum.a..n.. .P..e..r.c.e..p..t.i.o.n.. .o..f. .L.i.g..h..t. .In..t.e..n..s.i.t.y.. .a..n.d.. .C..o..l.o..r. .................................................................. 18 Light. .A...b.s..o..r.b..a.n..c..e.. .a.n..d.. .R..e..f.le..c..t.i.o.n.. ...................................................................................... 19 Fluo.r.e.s..c..e..n.c..e.. ................................................................................................................. 19 Phot.o..b..le..a..c..h.i.n..g.. .............................................................................................................. 20 Filte.r. .S..e..t.s. .f.o..r. .C..o..n..v.e..n.t.i.o..n..a.l. .v.s.... .M...u..lt.i.s..p..e.c..t.r.a..l. .Im...a..g..in..g.. ......................................................... 21 Mult.is..p..e..c.t.r.a..l. .A..n..a..ly..s..i.s. ..................................................................................................... 22 4 Wh..o..l.e. .S...li.d..e.. .S..c..a.n..n..i.n.g............................................................................................................. 24 5 Mu..lt.i.s..p..e.c..t.r.a..l. .F..ie..l.d.. .Im...a..g..in..g...................................................................................................... 25 Conf.i.g.u..r.i.n..g. .t.h..e.. .M...u.l.t.i.s..p.e..c..t.r.a..l .F..i.e..l.d.s.. .................................................................................. 26 Proc.e..s..s.i.n..g.. .t.h.e.. .M...u..lt.i.s..p..e.c..t.r.a..l. .F..ie..l.d..s. .................................................................................. 26 Chapter 4 Hardwa..r.e.. .R...e.f.e..r..e.n..c..e................................................................................. 27 1 Fro..n..t. .V..i.e.w.............................................................................................................................. 27 2 To.p. .V...ie..w................................................................................................................................ 29 3 Rig..h..t.-.S..i.d.e.. .V..i.e..w...................................................................................................................... 30 4 Le.ft.-.S...id..e.. .C..o..n..n.e..c..t.o..r.s.............................................................................................................. 31 5 Sli.d..e. .C...a.r.r.i.e..r. .H..o..t.e..l................................................................................................................. 32 6 Sli.d..e. .C...a.r.r.i.e..r.......................................................................................................................... 33 7 Ba.r.c..o..d.e.. .R..e..a..d..e.r..................................................................................................................... 35 8 Sy.s..t.e..m.. .C...o.m...p..u..t.e.r. .a..n..d.. .M..o..n..i.t.o.r................................................................................................ 35 Contents 3 9 Sp.e..c..if.i.c..a.t.i.o..n..s........................................................................................................................ 36 Chapter 5 Operat.i.o..n................................................................................................. 38 1 Sy.s..t.e..m.. .S...t.a.r.t.u..p...................................................................................................................... 38 Turn. .o.n.. .t.h..e.. .V..e..c.t.r.a.. .P..o..l.a..r.is.. .I.n..s.t.r.u..m...e..n.t. ............................................................................... 38 Laun.c..h.. .t.h..e. .V...e.c..t.r.a.. .P..o..l.a.r.i.s.. .S..o..f.t.w..a..r.e. .................................................................................. 39 2 Sy.s..t.e..m.. .S...h.u..t.d..o..w..n.................................................................................................................. 39 3 Ins.p..e..c..t.in..g.. .S..l.i.d.e..s.. .a..n.d.. .S..l.i.d..e. .C...a.r.r.i.e..r.s......................................................................................... 39 4 Lo.a.d..i.n..g. .S...li.d..e..s. .i.n..t.o.. .t.h.e.. .S..l.i.d..e. .C...a.r.r.i.e..r.s....................................................................................... 40 5 Lo.a.d..i.n..g. .S...li.d..e.. .C..a..r.r.ie..r.s.. .i.n.t.o.. .t.h..e.. .S..l.id..e.. .C..a..r.r.i.e.r. .H...o.t.e..l..................................................................... 41 6 Re.m...o..v.i.n.g.. .S..l.i.d..e. .C...a.r.r.i.e..r.s.. .fr.o..m... .t.h..e. .S...li.d..e.. .C..a..r.r.ie..r. .H..o..t.e..l................................................................. 43 7 Re.m...o..v.i.n.g.. .S..l.i.d..e.s.. .f.r.o..m.. .t.h..e.. .S..l.i.d.e.. .C..a..r.r.i.e..r.s................................................................................... 43 Chapter 6 Softwa.r.e.. .O...p..e.r..a..t.i.o..n.................................................................................. 44 1 So.f.t.w..a..r.e.. .O..v.e..r.v.i.e..w................................................................................................................... 44 Gear. .M...e..n..u. ..................................................................................................................... 46 2 Sy.s..t.e..m.. .D...a.s..h..b..o.a..r.d................................................................................................................. 47 Disk. .S..p..a..c.e.. .................................................................................................................... 48 Brigh..t.f.ie..l.d.. .R..e..fe..r.e..n..c.e..s.. ..................................................................................................... 48 Fluo.r.e.s..c..e..n.c..e.. .R..e..f.e.r.e..n..c..e.s.. ................................................................................................ 50 Com.p..e..n.s..a..t.i.o.n.. .I.n..fo..r.m...a..t.io..n.. ............................................................................................... 52 3 Cre..a..t.i.n.g.. .a..n..d. .E...d.i.t.i.n..g. .P...r.o.t.o..c..o..ls................................................................................................