Supplementary Table 1. miR-10b is predicted to target key molecules and pathways involved in carcinogenesis. Pathway Target gene name ECM-receptor interaction SDC1, COL24A1, COL4A4 NF-kappa B signaling pathway TAB1, CSNK2A2, UBE2I, IRAK4, MAP3K7 Toll-like receptor signaling pathway TAB1, IRAK4, MAP3K7 Glioma E2F3, CAMK2B NOD-like receptor signaling pathway TAB1, MAP3K7 Ubiquitin mediated proteolysis RNF7, UBE2I, ERCC8 Apoptosis DFFB, TP53, FASLG, BCL2L1, CAPN2, PRKX, ATM, IRAK4, PRKACG, PRKAR2A, TNFRSF10B, TNFRSF10D, BCL2, IL1RAP, CASP8, PIK3CA, PRKACA, APAF1, CHP, PIK3R3 Chronic myeloid leukemia E2F3, BCR, GRB2, TGFBR1, CBL, TP53, CDK6, BCL2L1, GAB2, PIK3CA, MDM2, SHC1, PIK3R3, CRK Regulation of cell proliferation E2F3, FOSL2, CDX2, PDGFB, OSMR, E2F7, ARNT2, RBM5, STRN, PTEN, S1PR2, CUL3, BDNF, SERPINE1, SHC1, ASPH, ITCH, SPN, CCDC88A, FOXJ1, RXRA, TP53, CDK6, IRS1, VASH2, RBBP9, VASH1, ADRB2, PDGFRA, MDM2, ADAMTS1, EIF2AK2, EIF5A2, ICOSLG, ING5, FGFR3, NDN, ST8SIA1, BCL2L1, CDH5, ARNT, LIF, VDR, HOXA3, AGGF1, TSPAN31, BCL2, BCL11B, NKX3-1, BCL6, CD28, NACC1, FLT1, NF2, JARID2, TBX5, TGFBR1, NF1, KLF11, SMAD2, IGF2, TAX1BP3, BTLA, HDAC4, LEPRE1, CNTF, NUP62, TSC1, ETS1, ID4, NR5A2, KLF4, KCTD11, NFIB Melanoma E2F3, PDGFB, PDGFRA, FGF11, TP53, FGF23, MDM2, PIK3CA, CDK6, CDH1, PIK3R3, PTEN MAPK signaling pathway FGFR3, PDGFB, GRB2, FGF11, FASLG, GNG12, SRF, PRKX, MAP3K7, PRKACG, BDNF, RAC3, MAP3K2, PRKACA, CHP, RAPGEF2, TGFBR1, NF1, TP53, FGF23, STK4, DUSP5, MAP4K4, RPS6KA2, MAPK14, PDGFRA, PLA2G3, CACNA1C, CRK, PLA2G2F Colorectal cancer DVL3, APC2, RAC3, GRB2, TGFBR1, BCL2, PDGFRA, TP53, FZD1, PIK3CA, SMAD2, PIK3R3, FZD4 Wnt signaling pathway DVL3, APC2, BTRC, CAMK2G, VANGL2, FZD1, TP53, SMAD2, FZD4, PRKX, MAP3K7, CTNNBIP1, PRKACG, RAC3, NFAT5, PRKACA, CAMK2B, CHP, TBL1X p53 signaling pathway CCNB3, TNFRSF10B, SERPINE1, CASP8, TP53, MDM2, CDK6, APAF1, PERP, PTEN, ATM Prostate Cancer E2F3, PDGFB, GRB2, CREB1, TP53, IGF2, PTEN, BCL2, PDGFRA, NKX3-1, PIK3CA, MDM2, PIK3R3 Cell adhesion molecules (CAMs) PVR, ICAM1, MPZL1, CADM1, NFASC, CDH1, CDH5, SDC1, PVRL1, ITGB8, ITGAV, NLGN4X, PVRL2, JAM3, ICOSLG, SPN, CD28 Renal cell carcinoma PAK7, EPAS1, PDGFB, ETS1, GRB2, ARNT2, PIK3CA, PIK3R3, CRK, RAPGEF1, ARNT 1 Small cell lung cancer COL4A4, E2F3, BCL2, ITGAV, RXRA, TP53, PIK3CA, CDK6, APAF1, BCL2L1, PIK3R3, PTEN Non-small cell lung cancer E2F3, GRB2, RXRA, TP53, PIK3CA, CDK6, FOXO3, PIK3R3, STK4 Adherens junction MAP3K7, ACTG1, PVRL1, RAC3, TGFBR1, PVRL2, CDH1, SMAD2, SSX2IP, MLLT4, VCL Angiogenesis NRP2, HTATIP2, FLT1, EPAS1, CSPG4, APOLD1, MMP14, SRF, PTEN, MAP3K7, SHB, PKNOX1, AGGF1, MAPK14, PLXDC1, CASP8, POFUT1 Pancreatic cancer E2F3, RAC3, TGFBR1, TP53, PIK3CA, CDK6, SMAD2, BCL2L1, PIK3R3, STAT3 Endometrial cancer APC2, GRB2, TP53, PIK3CA, CDH1, FOXO3, PIK3R3, PTEN Focal adhesion COL4A4, FLT1, PDGFB, GRB2, CAPN2, PTEN, COL5A1, VCL, ACTG1, PAK7, RAC3, ITGB8, ITGAV, BCL2, PDGFRA, GRLF1, PIK3CA, SHC1, PIK3R3, CRK, RAPGEF1 Regulation of cell migration PARD6B, ICAM1, FLT1, NF2, PDGFB, TBX5, NF1, ONECUT2, IGF2, IRS1, PTEN, VASH1, VCL, SPAG9, BCL2, PDGFRA, SCARB1, RNF20 Supplementary Figure 1. Validation of miR-10b downregulation in ccRCC compared to normal kidney tissue from the same patient. Representative bar graph showing miR-10b expression in ccRCC compared to normal counterpart from the same tissue. miR-10b was significantly downregulated in cancer compared to normal tissue (p <0.0001), in agreement with our RT-qPCR findings. 2 .