(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2019/014310 Al 17 January 2019 (17.01.2019) W !P O PCT (51) International Patent Classification: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, C07H 21/04 (2006.01) C12N 9/02 (2006.01) HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, C07K 14/415 (2006.01) CI2N 9/88 (2006.01) KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, C12N 1/16 (2006.01) CI2N 9/96 (2006.01) MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, C12N 1/19 (2006.01) C12N 15/09 (2006.01) OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, CI2N 1/21 (2006.01) C12N 15/52 (2006.01) SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, C12N S/10 (2006.01) TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (21) International Application Number: (84) Designated States (unless otherwise indicated, for every PCT/US2018/041579 kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, (22) International Filing Date: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 11 July 2018 ( 11.07.2018) TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, (25) Filing Language: English EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, (26) Publication Language: English TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, (30) Priority Data: KM, ML, MR, NE, SN, TD, TG). 62/532,297 13 July 2017 (13.07.2017) US Published: (71) Applicant: VERDEZYNE (ABC), LLC [US/US]; 2735 — with international search report (Art. 21(3)) Sand Hill Road, Menlo Park, CA 94025 (US). (72) Inventor: AELING, Kimbely, Ann; 324 Plaza Estival, San Clemente, CA 92672 (US). (74) Agent: DICKINSON, Kari A. et al; Grant IP, Inc., C/o CPA Global, 900 Second Avenue S., Suite 600, Minneapo lis, MN 55402 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (54) Title: BIOLOGICAL METHODS FOR PREPARING TERPENES FIG. 1 ura3 (non-functiona > © A) © B) -Μ Γ~ Γ - Γ ¾¾¾Γ «« - >l ~ i ' - o (57) Abstract: The technology relates in part to biological methods for producing terpenes and to engineered cells and microorganisms o capable of such production. BIOLOGICAL METHODS FOR PREPARING TERPENES Related Patent Applications This patent application claims the benefit of U.S. provisional patent application no. 62/532,297, filed on July 13, 2017, entitled BIOLOGICAL METHODS FOR PREPARING TERPENES, naming Kimberly Ann Aeling as inventor, and designated by Attorney Docket No. VRD-3002- PV. This patent application is related to an International patent application, filed simultaneously herewith, entitled BIOLOGICAL METHODS FOR MODIFYING CELLULAR CARBON FLUX, naming Tom Beardslee as inventor, and designated by Attorney Docket No. VAL-3001-PC, which claims the benefit of U.S. provisional patent application no. 62/532,292, filed on July 13, 2017, entitled BIOLOGICAL METHODS FOR MODIFYING CELLULAR CARBON FLUX, naming Tom Beardslee as inventor, and designated by Attorney Docket No. VRD-3001-PV. This patent application also is related to U.S. provisional patent application no. 61/222,902 filed on July 2 , 2009, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio as inventor, and designated by Attorney Docket No. VRD-1001-PV. This patent application also is related to International patent application no. PCT/US20 10/040837 filed on July 1, 2010, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-PC. This patent application also is related to U.S. provisional patent application no. 61/430,097 filed on January 5 , 201 1 , entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-PV2. This patent application also is related to U.S. provisional patent application no. 61/482,160 filed on May 3 , 201 1, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-PV3. This patent application also is related to U.S. patent application no. 13/245,777 filed on September 26, 201 1, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-CT. This patent application also is related to U.S. patent application no. 13/245,780 filed on September 26, 201 1 , entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD- 1001-UT. This patent application also is related to U.S. patent application no. 13/245,782 filed on September 26, 201 1 , entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-UT2. This patent application also is related to International patent application no. PCT/US201 2/020230 filed on January 4 , 2012, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1001-PC2. This patent application also is related to International patent application no. PCT/US20 12/056562 filed on September 2 1, 2012, entitled BIOLOGICAL METHODS FOR PREPARING ADIPIC ACID, naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD- 1001-PC3. This patent application also is related to U.S. provisional patent application no. 62/136,350 filed on March 20, 2015, entitled BIOLOGICAL METHODS FOR PREPARING 3- HYDROXYPROPIONIC ACID, naming Eric Michael Knight as inventor, and designated by Attorney Docket No. VRD-2001-PV. This patent application also is related to International patent application no. PCT/US201 6/023243 filed on March 18, 2016, entitled BIOLOGICAL METHODS FOR PREPARING 3-HYDROXYPROPIONIC ACID, naming Eric Michael Knight as inventor. This patent application also is related to U.S. provisional patent application no. 61/505,092 filed on July 6 , 201 1 , entitled BIOLOGICAL METHODS FOR PREPARING SEBACIC ACID naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1005-PV. This patent application also is related to U.S. provisional patent application no. 61/523,216 filed August 12, 201 1, entitled BIOLOGICAL METHODS FOR PREPARING DODECANEDIOIC ACID naming Stephen Picataggio and Tom Beardslee as inventors, and designated by Attorney Docket No. VRD-1006-PV. This patent application also is related to International patent application no. PCT/US201 2/04561 5 filed on July 5 , 2012, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Stephen Picataggio, L . Dudley Eirich and Jose Miguel Laplaza as inventors, and designated by Attorney Docket No. VRD-1005-PC. This patent application also is related to International patent application no. PCT/US20 12/045622 filed on July 5 , 2012, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Stephen Picataggio, Alex Hutagalung and Tom Fahland as inventors, and designated by Attorney Docket No. VRD-1006-PC. This patent application also is related to U.S. patent application no. 14/131,170 filed on April 14, 2014 entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Stephen Picataggio, L . Dudley Eirich and Jose Miguel Laplaza as inventors. This patent application also is related to U.S. patent application no. 14/131,174 filed on April 28, 2014, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Stephen Picataggio, Alex Hutagalung and Tom Fahland as inventors. This patent application also is related to U.S. provisional patent application no. 61/739,656 filed December 19, 2012, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID naming Jose Laplaza, Tom Beardslee, Dudley Eirich and Stephen Picataggio as inventors, and designated by Attorney Docket No. VRD-1007-PV. This patent application also is related to U.S. provisional patent application no. 61/739,661 filed December 19, 2012, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID naming Tom Beardslee, Alex Hutagalung and Stephen Picataggio as inventors, and designated by Attorney Docket No. VRD-1008-PV. This patent application also is related to International patent application no. PCT/US20 13/076664 filed on December 19, 2013, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Jose Laplaza, Tom Beardslee, Dudley Eirich and Stephen Picataggio as inventors. This patent application also is related to International patent application no. PCT/US20 13/076739 filed on December 19, 2013, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Alex Hutagalung and Stephen Picataggio as inventors. This patent application also is related to U.S. patent application no. 14/654,442 filed on June 19, 2015 entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Jose Laplaza, Tom Beardslee, Dudley Eirich and Stephen Picataggio as inventors. This patent application also is related to U.S. patent application no. 14/654,458 filed on June 19, 2015, entitled BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID, naming Tom Beardslee, Alex Hutagalung and Stephen Picataggio as inventors.