Image credits: Paul Danielson, Albany Research Center, OR D r. Michael W. Davidson, Florida State University D r. Rob We a v e r, McCrone Institute Joseph J. Buchino, M.D. Director of Pathology at Virginia Hospital D r. Catherine Carr, MBL,Woods Hole, MA DIAGNOSTIC INSTRUMENTS, INC 6540 Burroughs Street Sterling Heights, MI 48314 586.731.6000 • www.diaginc.com • Reflected Light • Brightfield • Phase • Fixed Cell Fluore s c e n c e • Metallurg y • Darkfield • DIC • Karyotyping • Semiconductor • Pathology • Hoff m a n • FISH High Res Phase Karyotype I n s p e c t i o n • Metro l o g y • Histology • Fore n s i c s • Gross Anatomy • Macro - p h o t o g r a p h y Best fit for application D a r k f i e l d • Machine Vi s i o n DIC Triple stain Suitable for application M e t a l l u rgy H&E slide | page 4 | page 5 | page 6 | page 7 | page 10- 1 1 2 | page 8-9 • Fluorescent Proteins • Chemiluminescence • FRET • FRAP • SMF • GFP • Bioluminescence • Ion Imaging • TIRF • Real-time Confocal • Two Photon • YFP • Gene Expression Pro f i l e s • Fura • Particle Tr a c k i n g • Whole Org a n i s m • Flow 4 • 4D- 6D Imaging GFP/Phase F l u o re s c e n c e • Quantum Dot Calcium Ratio Two Photon L u c i f e r a s e YFP F R E T Quantum Dot C o n f o c a l S M F C h e m i - g e l 3 I N S I G H T | unsurpassed value Key Features: I N S I G H T ™ cameras provide a feature packed imaging system for most documentation • Cost effective package/solutions applications. When you buy an I n s i g h t camera, you benefit from engineering expertise • High resolution replacement for video capture gained from Diagnostic Instruments’ re s e a rch and development into high-end quantitative • Real-time viewing camera designs. The result is low noise, high speed image captures that produce stunningly • Plug and play computer interface clean views of your specimen. Some of the true strengths of the I n s i g h t camera system • Powerful, easy to use software comes from the SPOT software that not only makes it easy to control the sophisticated • Color and monochrome models h a rd w a re, but takes you well beyond image capture. Powerful, yet intuitive software functions provide many microscopists with all the tools they will ever need. I n s i g h t gets your work done! F e a t u re s : B e n e f i t : 40 MHz live mode (dual channel 20 Mhz) Real-time viewing of specimens 14 bit x 20 Mhz capture High speed, low noise capture 1-20x programmable gain Facilitates low light imaging 2 & 4 Mpixel CCD sensors High resolution imaging 1” & 4/3” Format CCD sensors Get full field-of-view without expensive couplers High quantum efficiency CCD I n c reases sensitivity for faster capture s 1394a Fire W i re™ interface Fast, easy to install computer interface SPOT software Easy to use powerful imaging enviro n m e n t A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y P a t h o l o g y, Highest resolution, highest field-of-view IN 1430 E x c e l l e n t M e t a l l u rg y, Highest resolution, standard field-of-view IN 1830 E x c e l l e n t Color Brightfield S t a n d a rd resolution, highest field-of-view IN 1420 G o o d S t a n d a rd resolution, standard field-of-view IN 1820 G o o d Color Brightfield & S t a n d a rd resolution, highest field-of-view IN 1420 G o o d Bright Fluore s c e n c e S t a n d a rd resolution, standard field-of-view IN 1820 G o o d Visible Spectrum Highest resolution, highest field-of-view IN 1410 E x c e l l e n t M o n o c h rome Brightfield Highest resolution, standard field-of-view IN 1810 E x c e l l e n t B road Spectrum Highest resolution, highest field-of-view IN 1400 E x c e l l e n t M o n o c h rome Brightfield Highest resolution, standard field-of-view IN 1800 E x c e l l e n t DIC/Phase plus S t a n d a rd resolution, highest field-of-view IN 1420 G o o d Fixed Cell Fluore s c e n c e S t a n d a rd resolution, standard field-of-view IN 1820 G o o d 4 do what you want to do | F L E X Key Features: • Flexible resolution from high to ultra-high resolution FLEX™ Cameras combine acquisition flexibility with category leading specifications to • Phenomenal color from co-site sampling set the new benchmark for high-resolution documentation applications. With Flex shifting • Wide field-of-view sensor technology, you can select the resolution dependent on your needs: single-shot 4 MP • Highly cooled capture for live/moving specimens, 4-shot 4 MP full color sampling for high magnification- • Real-time viewing fixed specimens, 16-shot 16 MP for low magnification-fixed specimens, or a 64 MP color • Plug-and-play computer interface (using image double) mosaic image for smooth large format publication. • Powerful, easy to use software Flex monochrome goes even further, providing 9-shot, 36 MP resolution and 16-shot, 64 MP • Color model true resolution without image doubling. With the higher QE of the monochrome CCD and • Extreme resolution monochrome model 14-bit data capture, this is a perfect imaging camera for high resolution industrial inspection applications. Flex cameras are an excellent solution for capturing images of both brightfield and fluorescence specimens. Making all this power and flexibility easy is SPOT Software that combines real-time viewing and image capture with essential post processing, storage and simple automation. Flex lets you do just what you want to do. F e a t u re s : B e n e f i t : 4 Mpixel CCD sensors High resolution single-shot imaging Flex™ shifting sensor technology Ultra-high resolution multi-shot imaging Color co-site sampling Truest high resolution color 40 MHz live mode (dual channel 20 Mhz) Real-time viewing of specimens 14 bit x 20 Mhz capture High speed, low noise capture High quantum efficiency CCD I n c reases sensitivity for faster capture s - 2 0°C diff e rential cooling Facilitates extended exposure for low light imaging 1-20x programmable gain Facilitates low light imaging 4/3” format CCD sensors Full field-of-view without expensive couplers 1394a Fire W i re™ interface Fast, easy to install computer interface SPOT Software Easy to use, powerful imaging enviro n m e n t A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y P a t h o l o g y, Ultra-high resolution, highest field-of-view FX 1520 B e s t Color Brightfield & Fluore s c e n c e DIC/Phase plus Ultra-high resolution, highest field-of-view FX 1520 B e s t Fixed Cell Fluore s c e n c e M e t a l l u rg y FX 1520 B e s t FX 1500 G o o d I n s p e c t i o n E x t reme resolution, highest field-of-view FX 1500 B e s t 5 RT 3 | your new favorite Key Features: • Tremendous value • Low noise The SPOT RT 3™ digital camera system is the newest version from the immensely popular SPOT • Highly cooled, very low dark current RT™ line of cameras, combining improved performance with F i re W i re® connectivity. • FireWire connectivity With an effective diff e rential cooling of –43°C, and improved CCD sensor design, dark current has • Real-time viewing been suppressed to 0.003 e/p/s. This is a 20x improvement making the RT 3 an excellent • Powerful software support performer for longer exposure fluorescent applications. • Smaller size • Color, monochrome and slider Image acquisition rates have been tripled, while reducing read noise by over 25%, providing even (combined color and monochrome) models higher dynamic range when combined with the deep pixel wells of its Kodak KAI 2020 CCD. The RT 3 camera is off e red in monochrome, color mosaic, and user selectable color/monochro m e slider models, making this a great performer for both your documentation and quantitative re s e a rc h n e e d s . F e a t u re s : B e n e f i t : 2 Mpixel CCD sensors High resolution single shot imaging 16 e- read noise P recise readings for analytical applications - 4 3°C diff e rential cooling, dark current 0.003 e/p/s Major dark noise re d u c t i o n 14 bit x 20 Mhz capture Over 16,000 potential brightness levels I m p roved high quantum efficiency CCD I n c reases sensitivity for faster capture s High speed live mode Real-time viewing of specimens P rogrammable gain Facilitates low light imaging SPOT Software Easy to use, powerful imaging enviro n m e n t L a rge 3rd party driver selection Choice of application software F i re w i re (IEEE 1394a) Plug and play high speed connectivity A p p l i c a t i o n D e s c r i p t i o n M o d e l S u i t a b i l i t y R e s e a rch pathology RT 2540 E x c e l l e n t DIC plus RT 2500 E x c e l l e n t Fixed Cell Fluore s c e n c e F l u o rescence pro t e i n s Cooled high sensitivity RT 2500 Very Good G F P, YFP C o re lab Multiple application capability: Brightfield, RT 2540 E x c e l l e n t Fixed Cell Fluorescence, DIC, Phase Fixed Cell Fluore s c e n c e RT 2540 E x c e l l e n t 6 Key Features: see the world through a larger window | PURSUIT/XPLORER 4MP • Large field-of-view • High resolution • Low noise readout with optimized streaming mode • Software selectable readout speed PURSUIT™ 4MP cameras are solid, low noise cameras that provide a large field of view without need of • High dynamic range reduction optics.