TAXONOMIC DESCRIPTION Polburee et al., Int J Syst Evol Microbiol 2017;67:2377–2382 DOI 10.1099/ijsem.0.001961 List of Publications for 2017 Khanok RAAAKHAOKCHAI Nakazawaea todaengensis f.a., sp. nov., a yeast isolated from a 1. Phitsuwan, P., Permsriburasuk, C., Baramee, S., Teeravivattanakit, T., and peat swamp forest in Thailand Ratanakhanokchai, K. (2017). Structural analysis of alkaline pretreated rice straw for 1,5 1 2 3 1,4, ethanol production. International Journal of Polymer Science 17: Article ID Pirapan Polburee, Noppon Lertwattanasakul, Pitayakon Limtong, Marizeth Groenewald and Savitree Limtong * 4876969, 9 pages. https://doi.org/10.1155/2017/4876969. Abstract 2. Teeravivattanakit, T., Baramee, B., Phitsuwan, P., Sornyotha, S., Waeonukul, R., T Pason, P., Tachaapaikoon, C., Poomputsa, K., Kosugi, A., Sakka, K., and Strain DMKU-PS11(1) was isolated from peat in a swamp forest in Thailand. DNA sequence analysis showed that it belonged to a novel species that was most closely related to Nakazawaea laoshanensis. However, it differed from the type strain of Ratanakhanokchai, K. (2017). Chemical pretreatment-independent saccharifications N. laoshanensis (NRRL Y-63634T) by 2.3 % nucleotide substitutions in the D1/D2 region of the large subunit (LSU) rRNA gene, of xylan and cellulose of rice straw by bacterial weak lignin-binding xylanolytic and 1.0 % nucleotide substitutions in the small subunit (SSU) rRNA gene and 8.0 % nucleotide substitutions in the internal cellulolytic enzymes. Applied and Environmental Microbiology 83: no.22, transcribed spacer (ITS) region. The phylogenetic analyses based on the combined sequences of the SSU and the D1/D2 region e01522-17. and that of the SSU sequences alone confirmed the placement of the novel species in the Nakazawaea clade and its close affinity with N. laoshanensis. Hence, the species Nakazawaea todaengensis f.a., sp. nov. is proposed. The type strain is DMKU- T T T 3. Sonklin, C., Laohakunjit, N., Kerdchoechuen, O., and Ratanakhanokchai, K. (2018). PS11(1) (=CBS 14555 =TBRC 6559 ). The MycoBank number for Nakazawaea todaengensis f.a., sp. nov. is MB 819513 Volatile flavour compounds, sensory characteristics and antioxidant activities of mungbean meal protein hydrolysed by bromelain. Journal of Food Science and Technology 55: 265-277. DI 10.1007/s13197-017-2935-7. Nakazawaea is an ascomycetous yeast genus in the order N. wyomingensis [4]. One of these new combinations, N. Saccharomycetales. It was first proposed by Yamada et al. ernobii, which was suggested to be conspecific with N. hol- [1] to transfer Pichia holstii to a new genus, Nakazawaea, stii due to similar sequences of the D1/D2 region [5] showed 4. Apiwatanapiwat, W., Vaithanomsat, P., Thanapase, W., Ratanakhanokchai, K., and with N. holstii as the type species. N. holstii could be sepa- notable differences in the EF-1a, RPB1 and RPB2 sequences Kosugi, A. (2018). Xylan supplement improves 1,3-propanediol fermentation by rated from the other hat-shaped, ascospore-forming and [4]. However, Candida ernobii was listed as a synonym of Clostridium butyricum. Journal of Bioscience and Bioengineering 1: 662-668. nitrate-assimilating species of the genus Pichia based on the N. holstii in the latest version of The Yeasts, a Taxonomic partial sequences of the D1/D2 region of the large subunit Study [6]. Since then, one additional anamorphic species, N. (LSU) and the small subunit (SSU) rRNA genes. The pro- siamensis, which was isolated from the external surface of a 5. Aikawa, S., Baramee, S., Sermsathanaswadi, J., Thianheng, P., Tachaapaikoon, C., posal of this genus was later supported by phylogenetic sugarcane leaf in Thailand, was proposed by Kaewwichian Shikata, A., Waeonukul, R., Pason, P., Ratanakhanokchai, K., and Kosugi, A. (2018). analysis of the sequences of multiple protein-coding genes and Limtong [7]. Characterization and high-quality draft genome sequence of Herbivorax saccincola including actin (ACT1), the RNA polymerase largest sub- A tropical peat swamp forest is a unique ecosystem that is unit (RPB1) and second largest subunit (RPB2), the second A7, an anaerobic, alkaliphilic, thermophilic, cellulolytic, and xylanolytic bacterium. always flooded. Recent research showed the significance of Systematic and Applied Microbiology 41: 261-269. subunit of the mitochondrial cytochrome oxidase (COX2) and the D1/D2 region of the LSU rRNA gene [2]. According this ecosystem as a global carbon store for soil organic mat- to the new International Code of Nomenclature for algae, ter, but its value for maintaining biodiversity remains poorly fungi, and plants, it is possible to assign the teleomorphic understood [8]. Only a few yeasts isolated from peat lands and related anamorphic species to the same genus [3]. have been reported, of which most were obtained from peat – Therefore, 10 anamorphic species of the genus Candida in lands of Canada and Russia [9 12]. Recently, an investiga- the Nakazawaea clade were transferred to the genus Naka- tion on yeast diversity in peat of a swamp forest in the zawaea based on the phylogenetic analysis of the sequences southern part of Thailand was carried out by using an of the D1/D2 region of the LSU rRNA, the SSU rRNA, enrichment isolation technique [13]. In this investigation translation elongation factor-1a (EF-1a), and the RPB1 five species in the phylum Ascomycota, namely Cyberlind- and RPB2 gene regions. They were Nakazawaea anatomiae, nera subsufficiens, Debaryomyces fabryi, Meyerozyma guil- N. ernobii, N. ishiwadae, N. laoshanensis, N. molendini-olei, liermondii, Saturnispora diversa and Schwanniomyces N. peltata, N. pomicola, N. populi, N. wickerhamii and polymorphus var. africanus, and five species in the phylum Author affiliations: 1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, Thailand; 2Land Development Department, Ministry of Agriculture and Cooperatives, Bangkok, Thailand; 3Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands; 4Royal Society of Thailand, Bangkok, Thailand; 5Rattanakosin College for Sustainable Energy and Environment (RCSEE), Rajamangala University of Technology Rattanakosin, Nakhon Pathom, Thailand. *Correspondence: Savitree Limtong, [email protected] Keywords: Nakazawaea todaengensis sp. nov.; ascomycetous yeast; new species; peat; swamp forest; Thailand. Abbreviations: LSU, large subunit; SSU, small subunit. The GenBank/EMBL/DDBJ accession numbers of the SSU, the ITS region and the D1/D2 region of the LSU rRNA gene sequences of strain DMKU- PS11(1)T are LC171727, LC171728 and LC171729, respectively. The accession number for the SSU rRNA gene sequence of Nakazawaea siamensis is LC171726. The Mycobank number for Nakazawaea todaengensis f.a., sp. nov. is MB 819513. ã 001961 2017 IUMS Downloaded from www.microbiologyresearch.org by IP: 202.28.154.1802377 Reproduced from Int. J. Syst. Evol. Microbiol. 67: 2377-2382 (2017). On: Wed, 06 Sep 2017 02:54:43 112 113 Polburee et al., Int J Syst Evol Microbiol 2017;67:2377–2382 Basidiomycota, namely Piskurozyma taiwanensis, Cutaneo- PS11(1)T, which was isolated from a peat sample collected trichosporon mucoides, Papiliotrema flavescens, Papiliotrema from the rhizosphere of Calophyllum teysmannii var. inophyl- laurentii and Rhodotorula mucilaginosa were reported. loide at the sampling site (6 7¢ 52.3† N 101 58¢ 8.4† E) in the Su-ngai Padi district on 14 August 2015, was found to repre- In the current study, the yeast diversity in peat from a swamp sent a novel species of the genus Nakazawaea. forest in the Princess Sirindhorn Wildlife Sanctuary (Pru To Daeng Wildlife Sanctuary), that includes areas of four districts Genomic DNA extraction of strain DMKU-PS11(1)T was intheNarathiwatprovinceinthesouthernpartofThailand performed using the method described by Limtong et al. viz. Mueang, Tak Bai, Su-ngai Kolok and Su-ngai Padi, was [14]. The SSU rRNA gene, the ITS region and the D1/D2 investigated. During this investigation, a novel species of the region of the LSU rRNA gene were amplified using the pri- genus Nakazawaea was found. Yeasts were isolated from 12 mers SSU1f, SSU4r, SSU3f and SSU2r [15], ITS1 and ITS4 peat samples by using a dilution plate method. Peat (2 g) was [16], and NL1 and NL4 [5], respectively. The PCR products added to 50 ml 0.85 % saline solution in a 250 ml Erlenmeyer were purified by using the HiYield Gel/PCR DNA Frag- flask and mixed well by shaking on a rotary shaker at ments Extraction kit (RBC Bioscience) according to the 150 r.p.m. at room temperature (30±2 C) for 1 h. The solution manufacturer’s instructions. The purified products were (0.1 ml) was then spread on yeast malt extract (YM) agar sequenced by Macrogen (Seoul, Korea) using the PCR (0.3 % yeast extract, 0.3 % malt extract, 0.5 % peptone, 1 % glu- amplification primers. The sequences were edited, assem- cose and 2 % agar) supplemented with 0.025 % sodium propi- bled, concatenated and aligned with the MEGA software ver- onate and 0.02 % chloramphenicol and incubated at 25 C sion 7 [17]. In the present study, the SSU rRNA gene until yeast colonies appeared. Yeast colonies of different mor- sequence of N. siamensis [7] was also determined (Gen- phologies were picked and purified by cross streaking on YM Bank/EMBL/DDBJ accession number LC171726) and used agar. Purified yeast cultures were preserved in YM broth sup- in the phylogenetic analyses together with sequences plemented with 10 % (v/v) glycerol at À80 C. Fifty yeast obtained from GenBank (Fig. 1) for the additional species.