CHILEAN SOCIETY FOR CELL BIOLOGY XXV ANNUAL MEETING November, 1st – 5th, 2011 Puerto Varas SPONSORS CENTER FOR AGING AND REGENERATION (CARE) CENTRO DE ESTUDIOS MOLECULARES DE LA CELULA (CEMC) CENTRO FONDAP DE REGULACION DEL GENOMA FUNDACION CIENCIA PARA LA VIDA INSTITUTE FOR CELL DYNAMICS AND BIOTECHNOLOGY (ICDB) FUNDACION CHILENA PARA BIOLOGIA CELULAR MILLENNIUM INSTITUTE FOR FUNDAMENTAL AND APPLIED BIOLOGY (MIFAB) MILLENNIUM NUCLEUS ON IMMUNOLOGY AND IMMUNOTHERAPY (MNII) MILLENNIUM NUCLEUS IN REGENERATIVE BIOLOGY (MINREB) EXHIBITORS A. BRIL Y CIA LTDA – ALATHEIA MEDICA SA - ANDES IMPORT LTDA ARQUIMED SA – BD BIOSCIENCES – BIOSCHILE IG SA – CIENTEC SA – FERMELO SA GALENICA SA – GENESYS LTDA – GENEXPRESS – LABTEC SA LONCOTEC SA – MERCK QUIMICA DE CHILE – PERKIN ELMER CHILE ROCHE CHILE – TECNIGEN SA – TCL LTDA Chilean Society for Cell Biology – XXV Annual Meeting – November 1-5, 2011 1 INDEX CHILEAN SOCIETY FOR CELL BIOLOGY XXV ANNAUAL MEETING Program…………………………………………………………………………………………………2 Plenary Lectures…………………………………………………………………………………….....40 Symposiums……………………………………………………………………………………………44 Oral Presentations……………………………………………………………………………………..54 Poster Presentations I…………………………………………………………………………………73 Poster Presentations II……………………………………………………………………………….105 Poster Presentations III……………………………………………………………………………...137 Chilean Society for Cell Biology – XXV Annual Meeting – November 1-5, 2011 2 CHILEAN SOCIETY FOR CELL BIOLOGY XXV ANNUAL MEETING NOVEMBER, 1st-5th, 2011 PUERTO VARAS P R O G R A M Chilean Society for Cell Biology – XXV Annual Meeting – November 1-5, 2011 3 TUESDAY, NOVEMBER 1st, 2011 09:00 – 13:00 Registration Poster Mounting Session I: N° 1 to N° 85 Convention Center Foyer 11:30 – 12:30 Technical Lectures - Calbuco Room WITec GmbH and Genexpress Combining High Resolution Optical and Scanning Probe Microscopy Andrea Jaub. Senior Application Scientist, WITec GmbH, Germany Technical Lectures - Tronador Room Eppendorf and Arquimed Resource Optimization of PCR Equipment, Influence of Pipetting Techniques and Residues to Obtain Reproducible Results Josely Chiarela. Eppendorf, Brasil 12:30 – 13:30 Technical Lectures - Calbuco Room LifeTechnologies and GrupoBios SA Attune® Acoustic Focusing Cytometer and New Molecular Probes® Reagents for Cell Biology with a Focus on Violet Laser Capabilities Michael Olszowy, Director, Flow Cytometry Systems, LifeTechnologies 13:00 – 14:30 Lunch 14:30 – 15:30 Inauguration PLENARY LECTURE LUIS IZQUIERDO FERNANDEZ Volcanes Room - Chair: Maria Rosa Bono FROM RIBOSOMES TO PSYCHOSIS. Alfonso González. Departamento de Inmunología Clínica y Reumatología, Fac. Medicina. Centro de Envejecimiento y Regeneración (CARE). Fac. Ciencias Biológicas. Pontificia Universidad Católica de Chile. 15:30 – 17:30 Oral Presentations I Volcanes Room - Chair: Maria Rosa Bono and Co-Chair: Mauricio Gonzalez In vivo ANALYSIS OF CELLULAR TOXICITY MECHANISMS OF ALPHA-SYNUCLEIN AGGREGATES AND POTENTIAL THERAPEUTIC DRUGS IN Caenorhabditis elegans. A. Minniti4, I. Alfaro2, S. Bernales2,3 and R. Aldunate1. 1Escuela de Biotecnología, Universidad Santo Tomás, 2Fundación Ciencia para la Vida, 3Medivation Inc., 4Fac. Ciencias Biológicas, P. U. Católica de Chile. UNDERSTANDING THE NETWORK BETWEEN Th17 CELLS, REGULATORY T CELLS AND MYELOID DERIVED SUPPRESSOR CELLS IN THE TUMOR MICROENVIRONMENT. Sarah Núñez1, Juan José Sáez1, Mario Rosemblatt1,2, María Rosa Bono1 and Daniela Sauma1,2. 1Departamento de Biología, Facultad de Ciencias, Universidad de Chile; 2Fundación Ciencia para la Vida. [email protected] MEGALIN PROTEOLYTIC PROCESSING AND PHOSPHORYLATION IS AFFECTED BY OCRL1 DOWN REGULATION. Lisette Sandoval1, Antonella De Matteis2 and María Paz Marzolo1. 1Departamento de Biología Celular y Molecular, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile. 2Telethon Institute of Genetics and Medicine, 80131 Naples, Italy. [email protected] REPROGRAMMING OF A HUMAN CARCINOMA CELL LINE BY ZEBRAFISH EMBRYONIC MICROENVIRONMENTS. Leonel Muñoz, Germán Reig, and Miguel Concha. Laboratory of Experimental Ontogeny - LEO, ICBM, Faculty of Medicine, University of Chile; and Biomedical Neuroscience Institute, Santiago, Chile. [email protected]; [email protected] Chilean Society for Cell Biology – XXV Annual Meeting – November 1-5, 2011 4 LACONIC, A GENETICALLY ENCODED FRET NANOSENSOR FOR LACTATE. 1,2Alejandro San Martín, 1,2Sebastián Ceballo, 3Wolf B. Frommer and 1L. Felipe Barros. 1Centro de Estudios Científicos (CECs), Valdivia, 2Universidad Austral de Chile, Valdivia, & 3Carnegie Institution of Washington. [email protected] RUNX2 PROMOTES MIGRATION AND INVASION IN HUMAN OSTEOSARCOMA CELL LINES. Óscar Vega1,2, Claudia Lucero1,2, Julio Tapia2, Marcelo Antonelli2, Mercedes Lopez1,3, Flavio Salazar-Onfray1,3, Gary S Stein4, Andre van Wijnen4, Mario Galindo1,2. 1Millennium Institute on Immunology and Immunotherapy, 2Programa de Biología Celular y Molecular, 3Prode Inmunología, ICBM, Facultad de Medicina, Universidad de Chile. 4Department of Cell Biology and Cancer Center, University of Massachusetts Medical School, USA. [email protected] ROLE OF SYNDECAN-4 IN WNT/PLANAR CELL POLARITY PATHWAY DURING MOUSE DEVELOPMENT. Noelia Escobedo1, Marjorie Farías1, Osvaldo Contreras1, Hector Carrasco1, Uyen Tran2, Oliver Wessely2, Andrew Copp3 and Juan Larraín1. 1Center for Aging and Regeneration, Millennium Nucleus for Regenerative Biology, Faculty of Biological Sciences, P. Universidad Católica de Chile; 2Department of Cell Biology, Louisiana State University, Health Sciences Center, EEUU; 3Institute of Child Health, University College London,UK. [email protected] TESTOSTERONE INCREASES GLUCOSE UPTAKE THROUGH GLUT4 AND CaMKII/AMPK PATHWAY IN RAT CARDIOMYOCYTES. Carlos Wilson, Ariel Contreras, Katherine Montoya, Rodrigo Maass and Manuel Estrada. Facultad de Medicina, ICBM, Universidad de Chile. [email protected] 17:30 – 18:30 Coffee Break – Exhibitors – Poster Viewing: Session I Convention Center Foyer 18:30 – 19:30 PLENARY LECTURE CENTER FOR AGING AND REGENERATION (CARE) P. UNIVERSIDAD CATOLICA DE CHILE Volcanes Room - Chair: Nibaldo C. Inestrosa A NANOSCALE VIEW INTO THE DYNAMIC OF AMPA RECEPTOR ORGANIZATION IN SYNAPSES. Daniel Choquet. Institut Interdisciplinaire de Neuroscience, UMR 5297 CNRS-Université de Bordeaux. [email protected] 19:30 – 20:30 BEST THESES AWARDS FUNDACION CHILENA PARA BIOLOGIA CELULAR Volcanes Room - Chair: Federico Leighton and Co-Chair: Maria Rosa Bono UNDERGRADUATE FELIPE BELTRAN GONZALEZ, Biochemistry, Universidad Austral de Chile. GLUT3 is a key component in glucose transport modulation by ascorbic acid. Thesis Advisor: Maite Castro, PhD GRADUATE CARLA BITTNER HOFMANN, PhD in Sciences, Mention in Cell & Molecular Biology. Universidad Austral de Chile Acute modulation of astrocytic glucose metabolism by neuronal signals. Thesis Advisor: L. Felipe Barros, PhD 20:30 Dinner 22:00 – 23:30 Poster Presentations - Session I: N° 1 to N° 85 Convention Center Foyer Coordinators: Alejandra Alvarez, Maite Castro, Pedro Zamorano (1) HEMICHANNELS FORMED BY PANNEXIN1 ARE ACTIVATED BY EXTRACELLULAR ATP IN ADIPOCYTES. Fernández PE, Benvenuto MA, Sáez JC. Departamento de Fisiología, Pontificia Universidad Católica de Chile. [email protected] Chilean Society for Cell Biology – XXV Annual Meeting – November 1-5, 2011 5 (2) ROLE OF mTOR PATHWAY IN POST-MITOTIC TRANSLATION OF INHERITED RUNX2 MRNA IN MOUSE PRE-OSTEOBLASTS CELLS. Alejandra Aránguiz1, Nelson Varela1, Marcelo Antonelli1, Zhang Ying3, Gary Stein3, Andre van Wijnen3 y Mario Galindo1. 1Programa de Biología Celular y Molecular, ICBM, Facultad de Medicina, Universidad de Chile. 3Department of Cell Biology and Cancer Center, University of Massachutsetts Medical School, USA. [email protected] (3) ACTIVATION OF THE UNFOLDED PROTEIN RESPONSE (UPR) ENHANCES MOTOR RECOVERY AFTER SPINAL CORD INJURY. Vicente Valenzuela1,2,3, Eileen Collyer3, Donna Armentano4, Geoffrey Parsons4, Felipe A. Court3,5 and Claudio Hetz1,2,5. 1Biomedical Neuroscience Institute and 2Center for Molecular Studies of the Cell, Institute of Biomedical Sciences, University of Chile. 3Millennium Nucleus in Regenerative Biology (MINREB), Catholic University of Chile. 4Department of Molecular Biology, Genzyme Corporation, USA. 5Neurounion Biomedical Foundation. [email protected], [email protected] (4) PHOSPHOENOLPYRUVATE CARBOXYKINASE EXPRESSION IN Α CELLS FROM HUMAN AND MOUSE PANCREAS. Romina Bertinat1, Fabián Pardo1, Cristian Carrasco2, Karen Jaramillo1, Juan Carlos Slebe1 and Alejandro J. Yáñez1. Instituto de Bioquímica y Microbiología, Universidad Austral de Chile1; Unidad Anatomía Patológica, Hospital Base Valdivia2, Valdivia, Chile. [email protected] (5) ANALYSIS OF THE EPIGENETIC RESPONSE UNDER CONDITIONS OF CELLULAR ENERGY RESTRICTION DURING THE ACCLIMATIZATION OF C. carpio: THE eNosC COMPLEX. Fernández de la Reguera C., Nardocci G., Morales J., Molina A., Vera MI., Alvarez M. L. de Biología Celular y Molecular, F. de Ciencias Biológicas, U. Andrés Bello, Viña del Mar, Chile. [email protected]; [email protected] (6) TISSUE FACTOR PATHWAY INHIBITOR (TFPI) AND TISSUE FACTOR (TF) CO- LOCALIZE IN LIPID RAFTS (LR) OF PLATELET MEMBRANES: MECHANISM TO INHIBIT PLATELET PROCOAGULANT (PCA) ACTIVITY. González César, Matus Valeria, Pereira Jaime, Mezzano Diego, Panes Olga. Department of Hematology-Oncology, School of Medicine, P. Catholic University of Chile. [email protected] (Sponsor: V. Velarde) (7) INVOLVEMENT OF CONNEXIN26