10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 TNFAIP3 / A20 Antibody Goat Polyclonal Antibody Catalog # ALS13001 Specification TNFAIP3 / A20 Antibody - Product Information Application IHC Primary Accession P21580 Reactivity Human, Rabbit, Monkey Host Goat Clonality Polyclonal Calculated MW 90kDa KDa TNFAIP3 / A20 Antibody - Additional Information Gene ID 7128 Other Names Anti-TNFAIP3 antibody IHC of human small Tumor necrosis factor alpha-induced protein intestine. 3, TNF alpha-induced protein 3, 3.4.19.12, 6.3.2.-, OTU domain-containing protein 7C, Putative DNA-binding protein A20, Zinc TNFAIP3 / A20 Antibody - Background finger protein A20, A20p50, A20p37, TNFAIP3, OTUD7C Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Target/Specificity Involved in immune and inflammatory Human TNFAIP3. responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Reconstitution & Storage Toll-like receptors (TLRs) through terminating Store at -20°C. Minimize freezing and NF-kappa-B activity. Essential component of a thawing. ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures Precautions the transient nature of inflammatory signaling TNFAIP3 / A20 Antibody is for research use pathways. In cooperation with TAX1BP1 only and not for use in diagnostic or promotes disassembly of E2-E3 ubiquitin therapeutic procedures. protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 TNFAIP3 / A20 Antibody - Protein Information ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 Name TNFAIP3 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and Synonyms OTUD7C UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains Function on RIPK1 and catalyzes the formation of Ubiquitin-editing enzyme that contains both 'Lys-48'-polyubiquitin chains. This leads to ubiquitin ligase and deubiquitinase RIPK1 proteasomal degradation and activities. Involved in immune and consequently termination of the TNF- or inflammatory responses signaled by LPS-mediated activation of NF-kappa-B. Page 1/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 cytokines, such as TNF-alpha and IL-1 beta, Deubiquitinates TRAF6 probably acting on or pathogens via Toll-like receptors (TLRs) 'Lys-63'-linked polyubiquitin. Upon T-cell through terminating NF-kappa-B activity. receptor (TCR)-mediated T-cell activation, Essential component of a ubiquitin-editing deubiquitinates 'Lys-63'-polyubiquitin chains protein complex, comprising also RNF11, on MALT1 thereby mediating disassociation of ITCH and TAX1BP1, that ensures the the CBM (CARD11:BCL10:MALT1) and IKK transient nature of inflammatory signaling complexes and preventing sustained IKK pathways. In cooperation with TAX1BP1 activation. Deubiquitinates NEMO/IKBKG; the promotes disassembly of E2-E3 ubiquitin function is facilitated by TNIP1 and leads to protein ligase complexes in IL- 1R and inhibition of NF-kappa-B activation. Upon TNFR-1 pathways; affected are at least E3 stimulation by bacterial peptidoglycans, ligases TRAF6, TRAF2 and BIRC2, and E2 probably deubiquitinates RIPK2. Can also ubiquitin-conjugating enzymes UBE2N and inhibit I-kappa-B-kinase (IKK) through a UBE2D3. In cooperation with TAX1BP1 non-catalytic mechanism which involves promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and polyubiquitin; polyubiquitin promotes UBE2D3. Upon TNF stimulation, association with IKBKG and prevents IKK deubiquitinates 'Lys-63'-polyubiquitin MAP3K7-mediated phosphorylation. Targets chains on RIPK1 and catalyzes the TRAF2 for lysosomal degradation. In vitro able formation of 'Lys-48'-polyubiquitin chains. to deubiquitinate 'Lys-11'-, 'Lys-48'- and This leads to RIPK1 proteasomal 'Lys-63' polyubiquitin chains. Inhibitor of degradation and consequently termination programmed cell death. Has a role in the of the TNF- or LPS-mediated activation of function of the lymphoid system. Required for NF-kappa-B. Deubiquitinates TRAF6 LPS- induced production of proinflammatory probably acting on 'Lys-63'-linked cytokines and IFN beta in LPS-tolerized polyubiquitin. Upon T-cell receptor (TCR)- macrophages. mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 TNFAIP3 / A20 Antibody - References thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK Opipari A.W. Jr.,et al.J. Biol. Chem. complexes and preventing sustained IKK 265:14705-14708(1990). activation. Deubiquitinates NEMO/IKBKG; Ota T.,et al.Nat. Genet. 36:40-45(2004). the function is facilitated by TNIP1 and Mungall A.J.,et al.Nature 425:805-811(2003). leads to inhibition of NF-kappa-B activation. Mural R.J.,et al.Submitted (SEP-2005) to the Upon stimulation by bacterial EMBL/GenBank/DDBJ databases. peptidoglycans, probably deubiquitinates Bechtel S.,et al.BMC Genomics RIPK2. Can also inhibit I-kappa-B-kinase 8:399-399(2007). (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages. Cellular Location Cytoplasm. Nucleus. Lysosome. TNFAIP3 / A20 Antibody - Protocols Page 2/3 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 Provided below are standard protocols that you may find useful for product applications. • Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture Page 3/3 Powered by TCPDF (www.tcpdf.org).