UBE2E2 Antibody Cat. No.: 29-870 UBE2E2 Antibody Antibody used in WB on recombin ant protein at: 1:500 (Lanes: 1: 40ng HIS- UBE2D1 protein, 2: 40ng HIS- UBE2D2 protein, 3: 40ng HIS- UBE2D3 proteinm, 4: 40ng HIS- UBE2D4 protein, 5: 40ng HIS- UBE2E1 protein, 6: Antibody used in WB on Human Jurkat 0.125 ug/ml. 40ng HIS- UBE2E2 protein, 7: 40ng HIS- UBE2E3 protein, 8: 40ng HIS- UBE2K protein, 9: 40ng HIS- UBE2L3 protein, 10: 40ng HIS- UBE2N protein, 11: 40ng HIS- UBE2V1 protein, 12: 40ng HIS- UBE2V2 protein.). September 24, 2021 1 https://www.prosci-inc.com/ube2e2-antibody-29-870.html Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Human, Mouse, Rat Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human UBE2E2. TESTED APPLICATIONS: ELISA, IHC, WB UBE2E2 antibody can be used for detection of UBE2E2 by ELISA at 1:1562500. UBE2E2 APPLICATIONS: antibody can be used for detection of UBE2E2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 22 kDa WEIGHT: Properties PURIFICATION: Antibody is purified by peptide affinity chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store STORAGE CONDITIONS: UBE2E2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: UBE2E2 ALTERNATE NAMES: UBE2E2, FLJ25157, UBCH8 ACCESSION NO.: NP_689866 PROTEIN GI NO.: 22749327 GENE ID: 7325 USER NOTE: Optimal dilutions for each application to be determined by the researcher. September 24, 2021 2 https://www.prosci-inc.com/ube2e2-antibody-29-870.html Background and References BACKGROUND: UBE2E2 catalyzes the covalent attachment of ubiquitin to other proteins. REFERENCES: 1) Plafker, S.M., (2004) J. Cell Biol. 167 (4), 649-659. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 3 https://www.prosci-inc.com/ube2e2-antibody-29-870.html.