SunLong Biotech Co.,LTD Tel: 0086-571- 56623320 Fax:0086-571- 56623318 E-mail:[email protected] www.sunlongbiotech.com Rabbit Anti-Mitofilin antibody SL1824R Product Name: Mitofilin Chinese Name: Mitochondrion内膜蛋白抗体 Cell proliferation inducing protein 52; Cell proliferation-inducing gene 4/52 protein; HMP; IMMT; IMMT_HUMAN; Inner membrane protein mitochondrial; Inner Alias: mitochrondial membrane; MINOS2; Mitochondrial inner membrane organizing system 2; Mitochondrial inner membrane protein; Mitofilin; Motor protein; p87; p87/89; p89; pig4; PIG52; Proliferation-inducing gene 4; MICOS complex subunit MIC60. Organism Species: Rabbit Clonality: Polyclonal React Species: Human,Mouse,Rat,Dog,Cow, WB=1:500-2000ELISA=1:500-1000IHC-P=1:400-800IHC-F=1:400-800IF=1:100- 500（Paraffin sections need antigen repair） Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 68kDa Cellular localization: cytoplasmicThe cell membraneMitochondrion Form: Lyophilized or Liquid Concentration: 1mg/mlwww.sunlongbiotech.com immunogen: KLH conjugated synthetic peptide derived from human Mitofilin:241-340/758 Lsotype: IgG Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year Storage: when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed: PubMed Mitochondria are the center of cellular energy production and essential metabolic Product Detail: reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology. Mitofilin was originally described as heart muscle protein (HMP) because of its high expression in the heart. Subunit: Interacts with OPA1, preferentially with the soluble OPA1 form. Component of the MINOS/MitOS complex, that includes IMMT, HSPA9 and CHCHD3 and associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2. Interacts with CHCHD6; the interaction is direct. Subcellular Location: Mitochondrion inner membrane. SWISS: Q16891 Gene ID: 10989 Database links: Entrez Gene: 508853Cow Entrez Gene: 10989Human Entrez Gene: 76614Mouse Entrez Gene: 312444Rat Omim: 600378Human SwissProt:www.sunlongbiotech.com Q16891Human SwissProt: Q8CAQ8Mouse SwissProt: Q3KR86Rat Unigene: 148559Human Unigene: 235123Mouse Unigene: 47124Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Picture: Sample: A673(Human) Cell Lysate at 30 ug Primary: Anti-Mitofilin (SL1824R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution www.sunlongbiotech.comPredicted band size: 68 kD Observed band size: 68 kD Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (SL1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. www.sunlongbiotech.com Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Mitofilin) Polyclonal Antibody, Unconjugated (SL1824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. www.sunlongbiotech.com.