Keratin 7, 54 kD, clone RCK105 Monoclonal Antibody Catalog No.: MON3007 Quantity: 1 ml Specificity The monoclonal antibody RCK 105 is specifically reactive with keratin 7. In immunoblots, it reacts specifically with the 54 kD keratin protein (keratin 7). It shows cross reactivity with keratin 7 from human, hamster, mouse and potaroo kangaroo. Immunogen: cell line T24. Immunoglobulin type Murine IgG1 Use The antibody is useful for staining of Cytokeratin 7 in frozen sections. Cytokeratin 7 was found to occur in the columnar and glandular epithelium of the lung, cervix, breast, bile ducts, larger collecting ducts of the kidney and in mesothelium, but is absent from gastro-intestinal epithelium, hepatocytes, proximal and distal tubules of the kidney and myoepithelium. Keratin 7 could not be detected in the stratified epithelia of the skin, tongue, oesophagus or cervix but strongly stained urinary bladder transitional epithelium. The chain specific antibody to keratin 18 (M9) is able to distinguish between squamous cell carcinomas (M9 negative) on one hand, and adeno-carcinomas and mesotheliomas (M9 positive) on the other hand. The antibody to keratin 7 makes a subdivision within this latter group of tumors possible. Several types of adenocarcinomas of the gastro-intestinal tract and of the urinary tract are negative with RCK 105, while adenocarcinomas of the mammary gland, ovary and lung are positive. Instructions for use For immunohistochemistry on frozen sections fixation with acetone for 2 min. at -20°C is recommended. Working dilution: 1:5-1:10. Optimal dilution should be tested by serial dilutions. This antibody is also useful for Western blotting when applied undiluted although lesser amount of antibody could be sufficient therefore we recommend that each customer determines the optimal working dilution. As positive control for Western botting a preparation of HeLa-cells is recommended. Presentation 1 ml tissue culture supernatant in 0.15 M PBS with 1% BSA and 0.02% Na-azide. Literature - Ramaekers, F., et al., 1987, Exp. Cell Res., 170, 235. For application in tumor diagnosis see: - Fischer, H.P., 1987, Am. Journal of Pathology, 127, 530. - Asborn, M., et al., 1986, Lab. Invest. 55, 497. - Ramaekers, F., et al., 1990, Am. J. of Path. 136, 641-655. FOR RESEARCH USE ONLY, NOT FOR DRUG, DIAGNOSTIC OR OTHER USE. Cell Sciences, Inc. Toll Free: 888-769-1246 E-mail: [email protected] 480 Neponset Street Phone: 781-828-0610 Web Site: www.cellsciences.com Bldg 12A Fax: 781-828-0542 Canton, MA 02021 .