MDFI Antibody Cat. No.: 42-557 MDFI Antibody Specifications HOST SPECIES: Goat SPECIES REACTIVITY: HOMOLOGY: Expected Species Reactivity based on sequence homology: Human IMMUNOGEN: The immunogen for this antibody is: YQVSGQRPSGCD TESTED APPLICATIONS: ELISA Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments gave an approx 20kDa band in Human Skeletal Muscle lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to APPLICATIONS: NP_005577.1. The 20kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Properties Purified from goat serum by ammonium sulphate precipitation followed by antigen PURIFICATION: affinity chromatography using the immunizing peptide. CLONALITY: Polyclonal September 24, 2021 1 https://www.prosci-inc.com/mdfi-antibody-42-557.html CONJUGATE: Unconjugated PHYSICAL STATE: Liquid Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum BUFFER: albumin. Aliquot and store at -20°C. Minimize freezing and thawing. CONCENTRATION: 500 ug/mL STORAGE CONDITIONS: Aliquot and store at -20˚C. Minimize freezing and thawing. Additional Info OFFICIAL SYMBOL: MDFI MDFI, MyoD family inhibitor, I-MF, I-mfa, OTTHUMP00000196414, inhibitor of MyoD family ALTERNATE NAMES: a ACCESSION NO.: NP_005577.1 PROTEIN GI NO.: 5031905 GENE ID: 4188 ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 2 https://www.prosci-inc.com/mdfi-antibody-42-557.html.