Established: 1959. The year 2014 is the 56th anniversary of continuous publication ISSN 0036-4665 ISSN 1678-9946 on line EDITOR-IN-CHIEF EMERITUS EDITORS Prof. Dr. Thales F. de Brito Prof. Dr. Luis Rey (Founding Editor) Associate Editors: Prof. Dr. Pedro Paulo Chieffi Prof. Dr. Carlos da Silva Lacaz Prof. Dr. Thelma S. Okay EDITORIAL BOARD Alan L. de Melo (Belo Horizonte, MG) Fernando Montero-Gei (San José, Costa Rica) Maria I. S. Duarte (S. Paulo, SP) Alberto Duarte (S. Paulo, SP) Flair J. Carrilho (S. Paulo, SP) Maria L. Higuchi (S. Paulo, SP) Angela Restrepo M. (Medellin, Colombia) Gil Benard (S. Paulo, SP) Mario Mariano (S. Paulo, SP) Anna Sara S. Levin (S. Paulo, SP) Gioconda San-Blas (Caracas, Venezuela) Mirian N. Sotto (S. Paulo, SP) Antonio A. Barone (S. Paulo, SP) Govinda Visvesvara (Atlanta, USA) Moisés Goldbaum (S. Paulo, SP) Antonio Carlos Nicodemo (S. Paulo, SP) Heitor F. Andrade Jr. (S. Paulo, SP) Moysés Mincis (S. Paulo, SP) Antonio Sesso (S. Paulo, SP) Henrique L. Lenzi (Rio de Janeiro, RJ) Moysés Sadigursky (Salvador, BA) Antonio W. Ferreira (S. Paulo, SP) Hiro Goto (S. Paulo, SP) Myrthes T. Barros (S. Paulo, SP) Barnett L. Cline (New Orleans, USA) Ises A. Abrahamsohn (S. Paulo, SP) Nilma Cintra Leal (Recife, PE) Carlos F. S. Amaral (Belo Horizonte, MG) João Carlos Pinto Dias (Belo Horizonte, MG) Paulo C. Cotrim (São Paulo, SP) Celso Granato (S. Paulo, SP) João Renato Rebello Pinho (Sao Paulo, SP) Paulo M. Z. Coelho (Belo Horizonte, MG) Cesar A. Cuba Cuba (Brasília, DF) José Eduardo Levi (S. Paulo, SP) Regina Abdulkader (S. Paulo, SP) César Naquira V. (Lima, Peru) José M. R. Zeitune (Campinas, SP) Ricardo Negroni (B. Aires, Argentina) Clarisse M. Machado (S. Paulo, SP) Julia Maria Costa-Cruz (Uberlândia, MG) Robert H. Gilman (Baltimore, USA) Claudio S. Pannuti (S. Paulo, SP) Julio Litvoc (S. Paulo, SP) Roberto Martinez (Rib. Preto, SP) Cláudio Santos Ferreira (S. Paulo, SP) Luiz Carlos Severo (P. Alegre, RS) Semíramis Guimarães F. Viana (Botucatu, SP) Dalton L. F. Alves (Belo Horizonte, MG) Luiz Jacintho da Silva (Campinas, SP) Silvino A. Carvalho (S. Paulo, SP) Eridan Coutinho (Recife, PE) Luiz T. M. Figueiredo (Rib. Preto, SP) Silvio Alencar Marques (Botucatu, SP) Ernesto Hofer (Rio de Janeiro, RJ) Lygia B. Iversson (S. Paulo, SP) Sumie Hoshino-Shimizu (S. Paulo, SP) Euclides A. Castilho (S. Paulo, SP) Marcello Fabiano de Franco (S. Paulo, SP) Tsutomu Takeuchi (Tokyo, Japan) Eufrosina S. Umezawa (S. Paulo, SP) Marcos A. Rossi (Ribeirão Preto, SP) Venâncio A. F. Alves (S. Paulo, SP) Fan Hui Wen (S. Paulo, SP) Marcos Boulos (S. Paulo, SP) Vicente Amato Neto (S. Paulo, SP) Fernando A. Corrêa (S. Paulo, SP) M. A. Shikanai-Yasuda (S. Paulo, SP) Zilton A. Andrade (Salvador, BA) Executive Board - Librarians: Maria do Carmo Berthe Rosa; Sonia Pedrozo Gomes; Maria Ângela de Castro Fígaro Pinca; Carlos José Quinteiro The Revista do Instituto de Medicina Tropical de São Paulo is abstracted and/or indexed in: Index Medicus, Biological Abstracts, EMBASE/Excerpta Medica, Hepatology/Rapid Literature Review, Tropical Diseases Bulletin, Referativnyi Zhurnal: All-Russian Institute of Scientific and Technical Information (VINITI), Periódica - Índice de Revistas Latinoamericanas en Ciencias, Helminthological Abstracts, Protozoological Abstracts, Review of Medical and Veterinary Mycology, PubMed, UnCover, HealthGate, OVID, LILACS, MEDLINE, New Jour, ExtraMED, Free Medical Journals, ISI (Institute for Scientific Information), BIOSIS Previews, Scopus, Science Citation Index Expanded (SciSearch), Journal Citation Reports/Science Edition, Current Contents®/Clinical Medicine and Index Copernicus. ON LINE ACCESS - http://www.imt.usp.br/portal/ - FREE PDF ACCESS TO ALL PAST ISSUES, from 1959 on (Financial support by “Alves de Queiroz Family Fund for Research). http://www.scielo.br/rimtsp - FULL TEXT, SINCE 1984. E-mail: [email protected] Reprints may be obtained from Pro Quest Inf. and Learning, 300 North Zeeb Road, Ann Arbor, Michigan 48106-1346 - USA. The Revista do Instituto de Medicina Tropical de São Paulo is supported by: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Universidade de São Paulo and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). This issue was financed by: CNPq Proc. 403851/2012-2 and 405008/2013-9. Desktop Publishing by: Hermano - e-mail: [email protected]. Phone: 55.11.5571-8937. - Printed by: Elyon Indústria Gráfica, Phone: 55.11.3783-6527. English Revision: [email protected] UNIVERSIDADE DE SÃO PAULO - BRAZIL FACULDADE DE MEDICINA Instituto de Medicina Tropical de São Paulo Director: Prof. Dr. Paulo C. Cotrim I The purpose of the “Revista do Instituto de Medicina Tropical de São Paulo” (Journal of the São Paulo Institute of Tropical Medicine) is to publish the results of researches which contri- bute significantly to knowledge of all transmissible diseases. REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SÃO PAULO (JOURNAL OF THE S. PAULO INSTITUTE OF TROPICAL MEDICINE). São Paulo, SP-Brasil, 1959 - v. ilust. 28 cm 1959-2013, 1-55 1973-2002 (supl. 1-12) 2003 (supl. 13 - on-line only) 2005-2012 (supl. 14-18) 2014, 56 (1) ISSN 0036-4665 ISSN 1678-9946 on line II Impact Factor: 0.959 ISSN 0036-4665 ISSN 1678-9946 on line Rev. Inst. Med. Trop. Sao Paulo Vol. 56 No. 1 P. 1-92 January-February, 2014 CONTENTS REVIEW Animal models for the study of leishmaniasis immunology - E.N. LORÍA-CERVERA & F.J. ANDRADE-NARVÁEZ .................................................1 PLANT ANTIMICROBIAL ACTIVITY Evaluation of antimicrobial and cytotoxic activities of plant extracts from Southern Minas Gerais Cerrado - J.M. CHAVASCO, B.H.M. PRADO E FELIPHE, C.D. CERDEIRA, F.D. LEANDRO, L.F.L. COELHO, J.J. SILVA, J.K. CHAVASCO & A.L.T. DIAS ........................13 LEISHMANIASIS Distinct cellular migration induced by Leishmania infantum chagasi and saliva from Lutzomyia longipalpis in a hemorrhagic pool model - C.O. VASCONCELOS, Z.C.B. COÊLHO, C.S. CHAVES, C.R. TEIXEIRA, M.M.L. POMPEU & M.J. TEIXEIRA ..................................................21 MICROBIOLOGY Evaluation of four different DNA extraction methods in coagulase-negative staphylococci clinical isolates - C.F. OLIVEIRA, T.G.S. PAIM, K.C. REITER, A. RIEGER & P.A. D’AZEVEDO ...........................................................................................................................................................29 PARASITOLOGY Fasciola hepatica in bovines in Brazil: data availability and spatial distribution - S.C. BENNEMA, R.G.C. SCHOLTE, M.B. MOLENTO, C. MEDEIROS & O.S. CARVALHO ...............................................................................................................................................................................35 In vitro antigiardial activity of the cysteine protease inhibitor E-64 - T.B. CARVALHO, T.C.G. OLIVEIRA-SEQUEIRA & S. GUIMARÃES ..........43 Molecular typing of Giardia duodenalis isolates from nonhuman primates housed in a Brazilian zoo - E.B. DAVID, M. PATTI, S.T. CORADI, T.C.G. OLIVEIRA-SEQUEIRA, P.E.M. RIBOLLA & S. GUIMARÃES .......................................................................................................................49 URINARY TRACT INFECTION Frequency, urinalysis and susceptibility profile of pathogens causing urinary tract infections in Enugu State, Southeast Nigeria - U.M.E. DIBUA, I.S. ONYEMERELA & E.I. NWEZE ...............................................................................................................................................................................55 ANIMAL ENVENOMATION Coral snake antivenom produced in chickens (Gallus domesticus) - I. AGUILAR, E.E. SÁNCHEZ, M.E. GIRÓN, A. ESTRELLA, B. GUERRERO & F.A. RODRIGUEZ-ACOSTA ............................................................................................................................................................61 NOSOCOMIAL INFECTIONS First report of metallo-β-lactamases producing Enterobacter spp. strains from Venezuela - D. MARTÍNEZ, H.E. RODULFO, L. RODRÍGUEZ, L.E. CAÑA, B. MEDINA, M. GUZMAN, N. CARREÑO, D. MARCANO & M. DE DONATO..................................................................................67 Polyclonal outbreak of bloodstream infections caused by Burkholderia cepacia complex in Hematology and Bone Marrow Transplant outpatient units - I. BOSZCZOWSKI, G.V.B. PRADO, M.F. DALBEN, R.C.P. TELLES, M.P. FREIRE, T. GUIMARÃES, M.S. OLIVEIRA, J.F. ROSA, R.E. SOARES, P.E.D. LLACER, F.L. DULLEY, S.F. COSTA & A.S. LEVIN ................................................................................................................71 Enterococcus faecium and Enterococcus faecalis in blood of newborns with suspected nosocomial infection - I. FURTADO, P.C.N. XAVIER, L.V.M. TAVARES, F. ALVES, S.F. MARTINS, A.S. MARTINS & D.B. PALHARES ...................................................................................................77 CASE REPORT First case of autochthonous human visceral leishmaniasis in the urban center of Rio de Janeiro: case report - G.A.R. SILVA, T.O. BOECHAT, F.R.A. FERRY, J.F.C. PINTO, M.C.V.M. AZEVEDO, R.S. CARVALHO, R.N. MOTTA & M.F. VERAS ....................................................................81 Biopsy proven acute tubular necrosis due to rhabdomyolysis in a dengue fever patient: a case report and review of literature - L.P. REPIZO, D.M. MALHEIROS, L. YU, R.T. BARROS & E.A. BURDMANN ................................................................................................................................85