USOO842O367B2 (12) United States Patent (10) Patent No.: US 8.420,367 B2 Darzins et al. (45) Date of Patent: Apr. 16, 2013 (54) POLYNUCLEOTIDES ENCODING MUTANT 5,700,935 A 12/1997 Takenishi et al. HYDROLASE PROTEINS WITHENHANCED 3. A 6. 3. E. KINETICS AND FUNCTIONAL EXPRESSION 5,932,421war. A 8/1999 Ginsberga ca. et al. 5,945,526 A 8, 1999 Lee et al. (75) Inventors: Aldis Darzins, Highlands Ranch, CO 6.255.461 B1 7/2001 Mosbach et al. (US); Lance P. Encell, Fitchburg, WI 6,333,154 B1 12/2001 Ladant et al. (US); Rachel Friedman Ohana, 6,416,733 B1 7/2002 Barrett et al. Madison, WI (US); Paul Otto, Madison, g:25: R 1339: St. et al. WI (US); Gediminas Vidugiris, 6,800.453 B2 102004 Labaer et al. Fitchburg, WI (US); Keith V. Wood, Mt. 7,078,504 B2 7/2006 Short et al. Horeb, WI (US); Monika G. Wood, Mt. 7,238,842 B2 7/2007 Wood et al. Horeb, WI (US); Kris Zimmerman, 7,425,436 B2 9, 2008 Darzins et al. Madison, WI (US); Michael R. Slater, E. R. E. E." 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